Mouse Ephrin-A1 Antibody

Catalog # Availability Size / Price Qty
MAB2116
MAB2116-SP
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Mouse Ephrin-A1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Ephrin-A1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) Ephrin-A2 is observed, and no cross-reactivity with recombinant human Ephrin-A3, -B3, rmEphrin-A4, -A5, -B1, or -B2 is observed.
Source
Monoclonal Rat IgG2B Clone # 93036
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-A1
Ala18-Ser182 (predicted)
Accession # P52793
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Ephrin-A1 Fc Chimera (Catalog # 602-A1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A1

Ephrin-A1, also known as B61, LERK-1, and EFL-1, (1) is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A1 binds EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A1 share 85% amino acid identity. Only membrane‑bound or Fc‑clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). Nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/Ephrin families also appear to play a role in angiogenesis (3).

References
  1. Unified nomenclature for Eph family receptors and their ligands, the ephrins. Eph Nomenclature Committee [letter]. Cell (1997) 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen, The ephrins and Eph receptors in neural development. Annu. Rev. Neurosci. (1998) 21:309.
  3. Pasquale, E.B., The Eph family of receptors. Curr. Opin. Cell. Biol. (1997) 9:608.
Entrez Gene IDs
1942 (Human); 13636 (Mouse)
Alternate Names
B61; ECKLG; EFL1; EFL-1; EFNA1; EPH-related receptor tyrosine kinase ligand 1; EphrinA1; Ephrin-A1; EPLG1TNF alpha-induced protein 4; Immediate early response protein B61; LERK1; LERK1LERK-1; ligand of eph-related kinase 1; TNFAIP4B61; Tumor necrosis factor alpha-induced protein 4; tumor necrosis factor, alpha-induced protein 4

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