Mouse Ephrin-B1 Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse Ephrin‑B1 by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Mouse Ephrin-B1 Antibody Summary

Species Reactivity
Detects mouse Ephrin-B1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Ephrin-A1, -A2,
-A4, -B2, recombinant human Ephrin-A3, -A4, -A5, or -B3 is observed.
Monoclonal Rat IgG2A Clone # 94038
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-B1
Accession # AAA53231
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Ephrin-B1 antibody by Western Blot. View Larger

Detection of Mouse Ephrin‑B1 by Western Blot. Western blot shows lysates of mouse lung tissue and mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Ephrin-B1 Monoclonal Antibody (Catalog # MAB4731) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Ephrin-B1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-B1

Ephrin-B1, also known as LERK-2, ELK-L, EFL-3, Cek5-L, and STRA-1 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can become tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 amino acids long and are highly conserved, especially the last 33 amino acids. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B1 has been shown to bind EphA3, EphB1, EphB2, EphB3, and EphB4 (2, 3). The extracellular domains of human and mouse Ephrin-B1 share 94% amino acid identity. Only membrane‑bound or Fc-clustered ligands are capable of activating the receptor in vitro. Soluble monomeric ligands bind the receptor but do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Entrez Gene IDs
1947 (Human); 13641 (Mouse); 25186 (Rat)
Alternate Names
Cek5-L; EFL3; EFL-3; EFNB1; ELK ligand; ELK-L; EphrinB1; Ephrin-B1; LERK-2; STRA-1

Product Datasheets

You must select a language.


Citation for Mouse Ephrin-B1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function.
    Authors: Jin W, Qi S, Luo H
    BMC Immunol., 2011-12-19;12(0):68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse Ephrin-B1 Antibody

There are currently no reviews for this product. Be the first to review Mouse Ephrin-B1 Antibody and earn rewards!

Have you used Mouse Ephrin-B1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review