Mouse Epigen Antibody

U.S. Patent No. 6,150,502
Catalog # Availability Size / Price Qty
AF1127
AF1127-SP
Product Details
Citations (2)
FAQs
Supplemental Products
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Mouse Epigen Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Epigen in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Epigen
Leu53-Ala103
Accession # Q924X1.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Epigen (Catalog # 1127-EP)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse intestine and skin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Epigen

Epigen is an eipthelial mitogen that belongs to the EGF superfamily (1). Mouse Epigen cDNA encodes a 153 amino acid (aa) residue type I transmembrane precursor protein with a 18 aa putative signal peptide, a 92 aa extracellular domain, a 20 aa transmembrane domain, and a 22 aa cytoplasmic domain. Expression of mouse Epigen has been detected in multiple tissues including testis, liver and heart. Over the entire protein, mouse Epigen shares from approximately 23-33% sequence homology with other EGF family members. The functional internal peptide containing the EGF motif however is more than 40% homologous with that of TGF-alpha and epiregulin. A human cDNA clone encoding a mouse epigen-like protein has also been identified (2). Similarly to other EGF family proteins, a soluble form of mouse Epigen can most likely be generated from the membrane-bound form by proteolytic cleavage. Although recombinant mouse Epigen has been shown to be mitogenic for fibroblasts (3) and epithelial cells (1), compared to EGF and TGF-alpha, it is much less potent. The EGF receptor ErbB1 can be activated upon mouse Epigen activation. Nevertheless, involvements of additional ErbB receptors in mouse Epigen signaling have not been ruled out.

References
  1. Strachan, L. et al. (2001) J. Biol. Chem. 276:18265.
  2. Accession XM_171078.
  3. R&D Systems; unpublished results.
Entrez Gene IDs
255324 (Human); 71920 (Mouse)
Alternate Names
ALGV3072; EPG; EPGN; Epigen; PRO9904

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Product Specific Notices

U.S. Patent No. 6,150,502 used under license from Genesis Research and Development Corporation Limited

Citations for Mouse Epigen Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Aberrantly high expression of the CUB and zona pellucida-like domain-containing protein 1 (CUZD1) in mammary epithelium leads to breast tumorigenesis
    Authors: J Mapes, L Anandan, Q Li, A Neff, CV Clevenger, IC Bagchi, MK Bagchi
    J. Biol. Chem., 2018-01-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. The complete family of epidermal growth factor receptors and their ligands are co-ordinately expressed in breast cancer
    Authors: Emmet McIntyre, Edith Blackburn, Philip J. Brown, Colin G. Johnson, William J. Gullick
    Breast Cancer Research and Treatment

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