Mouse Epiregulin Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Epiregulin in ELISAs. In direct ELISAs, 100% cross‑reactivity with recombinant human Epiregulin is observed, but no cross‑reactivity with recombinant mouse (rm) Epigen, recombinant human (rh) TGF-alpha, rhAmphiregulin, rmBetacellulin, rhEGF, rhHB-EGF, rhHRG-alpha, or rhHRG-beta is observed. In sandwich immunoassays, no cross‑reactivity with rmAmphiregulin, rhNRG-1-beta 1, rmBetacellulin, rmEGF, rhHeregulin, rhHB-EGF, or rhTGF‑ alpha is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 189611
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Epiregulin
    Val56-Leu101
    Accession # Q61521
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Mouse Epiregulin Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse Epiregulin Antibody (Catalog # MAB1068)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Epiregulin Biotinylated Antibody (Catalog # BAF1068)
  • ELISA Standard
     
    Recombinant Mouse Epiregulin Protein (Catalog # 1068-EP)
  • Neutralization
    Measured by its ability to neutralize Epiregulin-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 3 ng/mL Recombinant Mouse Epiregulin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by Epiregulin and Neutralization by Mouse Epiregulin Antibody. Recombinant Mouse Epiregulin (Catalog # 1068-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Epiregulin (3 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti‑Mouse Epiregulin Monoclonal Antibody (Catalog # MAB1068). The ND50 is typically 0.2‑1 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Epiregulin

Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. They are all synthesized as transmembrane precursors and converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The mouse Epiregulin cDNA encodes for a 162 amino acid (aa) transmembrane precursor, with the mature soluble form comprising aa 56‑101 (2). The mode of action of Epiregulin is similar to other EGF family members in that it binds to and activates the tyrosine-kinase, ErbB-family receptors (ErbB1 through B4) (3). Although it stimulates phosphorylation of all four receptors, it appears to interact primarily with ErbB1 and ErbB4. Epiregulin has the broadest specificity of the EGF-like ligands but seems to preferentially activate heterodimeric receptor complexes (4). Epiregulin exhibits a variety of biological effects. It was originally shown to both inhibit growth of several epithelial tumor cells and stimulate growth of fibroblasts and other types of cells (1). Epiregulin expression is upregulated in a number of carcinoma cell lines. It has also been shown to be an autocrine growth factor in human epidermal keratinocytes (5). Epiregulin has also been shown to play a role in the early steps of pregnancy, regulating attachment of the blastocyst to the uterine epithelium during the implantation process (6).

  • References:
    1. Toyoda, H. et al. (1995) J. Biol. Chem. 270:7495.
    2. Toyoda, H. et al. (1995) FEBS Lett. 377:403.
    3. Komurasaki, T. et al. (1997) Oncogene 15:2841.
    4. Shelly, M. et al. (1998) J. Biol. Chem. 273:10496.
    5. Shirakata, Y. et al. (2000) J. Biol. Chem. 275:5748.
    6. Das, S.K. et al. (1997) Dev. Biol. 190:178.
  • Entrez Gene IDs:
    2069 (Human); 13874 (Mouse)
  • Alternate Names:
    Epiregulin; ER; EREG; proepiregulin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
    Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
    J Immunol, 2015;194(3):1039-46.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Isotype Controls
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Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2A Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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