Detects mouse Epiregulin in ELISAs. In direct ELISAs, 100% cross‑reactivity with recombinant human Epiregulin is observed, but no cross‑reactivity with recombinant mouse (rm) Epigen, recombinant human (rh) TGF-alpha, rhAmphiregulin, rmBetacellulin, rhEGF, rhHB-EGF, rhHRG-alpha, or rhHRG-beta is observed. In sandwich immunoassays, no cross‑reactivity with rmAmphiregulin, rhNRG-1-beta 1, rmBetacellulin, rmEGF, rhHeregulin, rhHB-EGF, or rhTGF‑ alpha is observed.
Monoclonal Rat IgG2A Clone # 189611
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Epiregulin Val56-Leu101 Accession # Q61521
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Mouse Epiregulin Protein (Catalog #
Measured by its ability to neutralize Epiregulin-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 3 ng/mL Recombinant Mouse Epiregulin.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Cell Proliferation Induced by Epiregulin and Neutralization by Mouse Epiregulin Antibody.
Recombinant Mouse Epiregulin (Catalog # 1068-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Epiregulin (3 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti‑Mouse Epiregulin Monoclonal Antibody (Catalog # MAB1068). The ND50 is typically 0.2‑1 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Epiregulin is a member of the EGF family of growth factors which includes, among others, epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, amphiregulin (ARG), HB (heparin-binding)-EGF, betacellulin, and the various heregulins. They are all synthesized as transmembrane precursors and converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7 (1). The mouse Epiregulin cDNA encodes for a 162 amino acid (aa) transmembrane precursor, with the mature soluble form comprising aa 56‑101 (2). The mode of action of Epiregulin is similar to other EGF family members in that it binds to and activates the tyrosine-kinase, ErbB-family receptors (ErbB1 through B4) (3). Although it stimulates phosphorylation of all four receptors, it appears to interact primarily with ErbB1 and ErbB4. Epiregulin has the broadest specificity of the EGF-like ligands but seems to preferentially activate heterodimeric receptor complexes (4). Epiregulin exhibits a variety of biological effects. It was originally shown to both inhibit growth of several epithelial tumor cells and stimulate growth of fibroblasts and other types of cells (1). Epiregulin expression is upregulated in a number of carcinoma cell lines. It has also been shown to be an autocrine growth factor in human epidermal keratinocytes (5). Epiregulin has also been shown to play a role in the early steps of pregnancy, regulating attachment of the blastocyst to the uterine epithelium during the implantation process (6).
Toyoda, H. et al. (1995) J. Biol. Chem. 270:7495.
Toyoda, H. et al. (1995) FEBS Lett. 377:403.
Komurasaki, T. et al. (1997) Oncogene 15:2841.
Shelly, M. et al. (1998) J. Biol. Chem. 273:10496.
Shirakata, Y. et al. (2000) J. Biol. Chem. 275:5748.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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