Mouse Erythropoietin/EPO Antibody
Mouse Erythropoietin/EPO Antibody Summary
Ala27-Arg192
Accession # P07321
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Mouse Erythropoietin/EPO Antibody. Recombinant Mouse Erythropoietin/EPO (Catalog # 959-ME) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin/EPO (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB959). The ND50 is typically 0.2-0.8 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Erythropoietin/EPO
Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of EPO is required for biological activities in vivo (4). Mature mouse EPO shares 95% amino acid sequence identity with rat EPO and 73-82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).
- Koury, M.J. (2005) Exp. Hematol. 33:1263.
- Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849.
- Wen, D. et al. (1993) Blood 82:1507.
- Tsuda E. et al. (1990) Eur. J. Biochem. 188:405.
- Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
- Eckardt, K.U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
- Sharples, E.J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
- Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
- Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
- Acs, G. et al. (2001) Cancer Res. 61:3561.
- Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
- Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
- Kertesz, N. et al. (2004) Dev. Biol. 276:101.
Product Datasheets
Citations for Mouse Erythropoietin/EPO Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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HIF prolyl hydroxylase inhibition protects skeletal muscle from eccentric contraction-induced injury
Authors: AN Billin, SE Honeycutt, AV McDougal, JP Kerr, Z Chen, JM Freudenber, DK Rajpal, G Luo, HF Kramer, RS Geske, F Fang, B Yao, RV Clark, J Lepore, A Cobitz, R Miller, K Nosaka, AC Hinken, AJ Russell
Skelet Muscle, 2018-11-13;8(1):35.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Stress-associated erythropoiesis initiation is regulated by type 1 conventional dendritic cells.
Authors: Kim T, Hanak M, Trampont P, Braciale T
J Clin Invest, 2015-09-21;125(10):3965-80.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Transforming growth factor-beta superfamily ligand trap ACE-536 corrects anemia by promoting late-stage erythropoiesis.
Authors: Suragani R, Cadena S, Cawley S, Sako D, Mitchell D, Li R, Davies M, Alexander M, Devine M, Loveday K, Underwood K, Grinberg A, Quisel J, Chopra R, Pearsall R, Seehra J, Kumar R
Nat Med, 2014-03-23;20(4):408-14.
Species: Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Rat
Sample Types: In Vivo
Applications: Neutralization -
Innate immune activation during Salmonella infection initiates extramedullary erythropoiesis and splenomegaly.
Authors: Jackson A, Nanton MR, O'Donnell H
J. Immunol., 2010-10-15;185(10):6198-204.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
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