Mouse Exostosin-like 1/EXTL1 Antibody

Catalog # Availability Size / Price Qty
MAB4214
MAB4214-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Mouse Exostosin-like 1/EXTL1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Exostosin-like 1/EXTL1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) EXTL1, rhEXTL2, rhEXTL3, or recombinant mouse EXTL2 is observed.
Source
Monoclonal Rat IgG2A Clone # 353713
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Exostosin-like 1/EXTL1
Ala30-Pro669
Accession # Q9JKV7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Exostosin-like 1/EXTL1
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Exostosin-like 1/EXTL1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Exostosin-like 1/EXTL1

Exostosin-like 1 (EXTL1) is a type II Golgi membrane protein and a glycosyl transferase that is required for the biosynthesis of heparan sulfate. The amino acid sequence of mouse EXTL1 is 92%, 76%, 75% and 74% identical to that of rat, chimpanzee, human and canine.

Entrez Gene IDs
2134 (Human); 56219 (Mouse); 313610 (Rat)
Alternate Names
alpha-N-acetylglucosaminyltransferase II; EC 2.4.1.224; exostoses (multiple)-like 1; Exostosin like 1; Exostosin-L; Exostosin-like 1; EXTL1; EXTLexostosin-like 1; glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase; Glucuronosyl-N-acetylglucosaminyl-proteoglycan4-alpha-N-acetylglucosaminyltransferase; glucuronyl-N-acetylglucosaminylproteoglycan alpha-1,4-N-acetylglucosaminyltransferase; MGC70794; Multiple exostosis-like protein

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Citations for Mouse Exostosin-like 1/EXTL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Glycosyltransferase Extl1 promotes CCR7-mediated dendritic cell migration to restrain infection and autoimmunity
    Authors: J Liu, Y Cheng, X Zhang, Y Chen, H Zhu, K Chen, S Liu, Z Li, X Cao
    Cell Reports, 2023-01-18;42(1):111991.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Glycotranscriptome study reveals an enzymatic switch modulating glycosaminoglycan synthesis during B-cell development and activation.
    Authors: Duchez S, Pascal V, Cogne N, Jayat-Vignoles C, Julien R, Cogne M
    Eur. J. Immunol., 2011-11-10;41(12):3632-44.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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