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Mouse FABP4/A-FABP Antibody

Catalog #: MAB1443
2 Citations Datasheet / COA / SDS

Discontinued Product

MAB1443 has been discontinued.
View all FABP4/A-FABP products.
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Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FABP4/A-FABP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FABP4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human FABP3 and no cross-reactivity with recombinant rat (rr) FABP1, rrFABP2, or recombinant mouse FABP5 is observed.
Source
Monoclonal Rat IgG2A Clone # 258417
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse FABP4
Met1-Ala132
Accession # P04117
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse FABP4
Immunocytochemistry
8-25 µg/mL
Immersion fixed ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes using Human/Mouse StemXVivo™ Osteogenic/Adipogenic Base Media (Catalog # CCM007) and Human/Mouse StemXVivo Adipogenic Supplement (Catalog # CCM011)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP4/A-FABP

FABP4, also known as adipocyte P2, is expressed in adipocytes and monocyte-derived foam cells. FABP4 binds free fatty acids and is involved in intracellular lipid transport. It has been shown to affect insulin sensitivity, lipid metabolism, and the development of atherosclerosis.

Long Name
Fatty Acid-Binding Protein 4
Entrez Gene IDs
2167 (Human); 11770 (Mouse); 79451 (Rat)
Alternate Names
Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; AFABP; A-FABP; A-FABPAFABP; ALBP; aP2; FABP4; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte

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Citations for Mouse FABP4/A-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The transcription factor NKX1-2 promotes adipogenesis and may contribute to a balance between adipocyte and osteoblast differentiation
    Authors: N Chen, RL Schill, M O'Donnell, K Xu, DP Bagchi, OA MacDougald, RJ Koenig, B Xu
    J. Biol. Chem., 2019-10-15;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. The microRNA miR-8 is a conserved negative regulator of Wnt signaling.
    Authors: Kennell JA, Gerin I, MacDougald OA, Cadigan KM
    Proc. Natl. Acad. Sci. U.S.A., 2008-09-29;105(40):15417-22.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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