Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody
R&D Systems | Catalog # BAF1460
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
Thr40-Arg217
Accession # NP_034317
Thr40-Arg217
Accession # NP_034317
Specificity
Detects mouse Fc gamma RII/RIII (CD32/CD16) in ELISAs and Western blots. In sandwich immunoassays,
less than 10% cross-reactivity with recombinant mouse CD16 is observed and less than 0.1% cross-reactivity with recombinant human CD32 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Fc gamma RIIB/CD32b (Catalog # 1460-CD)
Sample: Recombinant Mouse Fc gamma RIIB/CD32b (Catalog # 1460-CD)
Mouse Fc gamma RIIA/CD32a Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Fc gamma RII/RIII (CD32/CD16)
Additional Fc gamma RII/RIII (CD32/CD16) Products
Product Documents for Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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