Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1460
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Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Fc gamma RII/RIII (CD32/CD16) in ELISAs and Western blots. In sandwich immunoassays, less than 10% cross-reactivity with recombinant mouse CD16 is observed and less than 0.1% cross-reactivity with recombinant human CD32 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
Thr40-Arg217
Accession # NP_034317
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Fc gamma  RIIB/CD32b (Catalog # 1460-CD)

Mouse Fc gamma RIIA/CD32a Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Fc gamma  RIIB/CD32b Antibody (Catalog # MAB14601)

Standard: Recombinant Mouse Fc gamma RIIB/CD32b Protein, CF (Catalog # 1460-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RII/RIII (CD32/CD16)

Fc gamma RII, also named CD32, is a low-affinity receptor for the Fc region of complexed IgG. Three genes for human Fc gamma RII (A, B and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins, have been identified. The extracellular domain of mouse Fc gamma RII shares approximately 95% amino acid sequence identity with that of mouse Fc gamma RIII/CD16.

Long Name
Fc gamma Receptor II/III
Alternate Names
CD32/CD16; Fc gamma RII/RIII

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