Mouse Fc gamma RII/RIII (CD32/CD16) Antibody

Catalog # Availability Size / Price Qty
AF1460
AF1460-SP
Mouse Fc gamma  RII/RIII (CD32/CD16) Antibody in Data
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Citations (4)
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Mouse Fc gamma RII/RIII (CD32/CD16) Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Fc gamma RII/RIII (CD32/CD16) in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human Fc gamma RII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
Thr40-Arg217
Accession # P08101
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Fc gamma  RIIB/CD32b (Catalog # 1460-CD)
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Fc? RII/RIII (CD32/CD16) antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Fc gamma RII/RIII (CD32/CD16) in Mouse Splenocytes. Fc gamma RII/RIII (CD32/CD16) was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Fc gamma RII/RIII (CD32/CD16) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1460) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RII/RIII (CD32/CD16)

Fc gamma RII, also named CD32, is a low-affinity receptor for the Fc region of complexed IgG. Three genes for human Fc gamma RII (A, B and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins, have been identified. The extracellular domain of mouse Fc gamma RII shares approximately 95% amino acid sequence identity with that of mouse Fc gamma RIII/CD16.

Long Name
Fc gamma Receptor II/III
Alternate Names
CD32/CD16; Fc gamma RII/RIII

Product Datasheets

Citations for Mouse Fc gamma RII/RIII (CD32/CD16) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. FGF21 alleviates neuroinflammation following ischemic stroke by modulating the temporal and spatial dynamics of microglia/macrophages
    Authors: D Wang, F Liu, L Zhu, P Lin, F Han, X Wang, X Tan, L Lin, Y Xiong
    J Neuroinflammation, 2020;17(1):257.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. A purified capsular polysaccharide markedly inhibits inflammatory response during endotoxic shock.
    Authors: Piccioni M, Monari C, Kenno S, Pericolini E, Gabrielli E, Pietrella D, Perito S, Bistoni F, Kozel T, Vecchiarelli A
    Infect Immun, 2013;81(1):90-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
    Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
    Diabetologia, 2011;54(10):2713-23.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. New asthma biomarkers: lessons from murine models of acute and chronic asthma.
    Authors: Di Valentin E, Crahay C, Garbacki N, Hennuy B, Gueders M, Noel A, Foidart JM, Grooten J, Colige A, Piette J, Cataldo D
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(2):L185-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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