Mouse Fc gamma  RIIB/CD32b Antibody

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Product Details
Citations (4)
Supplemental Products
Reviews (1)

Mouse Fc gamma  RIIB/CD32b Antibody Summary

Species Reactivity
Detects mouse Fc gamma RIIB in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse (rm) Fc gamma RIII. In ELISAs, this antibody shows approximately 7% cross-reactivity with rmFc gamma  RIII.
Monoclonal Rat IgG2A Clone # 190907
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
aa 40-217
Accession # P08101
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse Fc gamma  RIIB/CD32b (Catalog # 1460-CD)
under non-reducing conditions only

Mouse Fc gamma RIIA/CD32a Sandwich Immunoassay

Recommended Concentration
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody (Catalog # BAF1460)

Standard: Recombinant Mouse Fc gamma RIIB/CD32b Protein, CF (Catalog # 1460-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIB/CD32b

Fc gamma RIIB, also known as CD32b, is a low affinity inhibitory receptor for the Fc portion of the IgG molecules. Fc gamma RIIB is expressed on B cells, macrophages, neutrophils, and mast cells and is involved in phagocytosis of immune complexes and modulation of antibody production by B cells.

Long Name
Fc gamma Receptor II
Entrez Gene IDs
2213 (Human); 14130 (Mouse); 289211 (Rat); 102139530 (Cynomolgus Monkey)
Alternate Names
CD32 antigen; CD32b; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RIIB; FCG2; Fc-gamma RII-b; fc-gamma-RIIb; FCGR2; FCGR2B; FcgRIIB; FCRIIB; fcRII-b; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b

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Citations for Mouse Fc gamma  RIIB/CD32b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Sex-specific accelerated decay in time/activity-dependent plasticity and associative memory in an animal model of Alzheimer's disease
    Authors: S Navakkode, JR Gaunt, MV Pavon, VA Bansal, RP Abraham, YS Chong, TH Ch'ng, S Sajikumar
    Aging Cell, 2021-11-18;0(0):e13502.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
    Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
    Cell Rep, 2019-06-11;27(11):3117-3123.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Functional Assay
  3. Osteoclast inhibitory peptide-1 binding to the Fc gammaRIIB inhibits osteoclast differentiation.
    Authors: Shanmugarajan S, Beeson CC, Reddy SV
    Endocrinology, 2010-07-07;151(9):4389-99.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Co-Immunoprecipitation, Western Blot
  4. Altered balance of inhibitory and active Fc gamma receptors in murine autoimmune glomerulonephritis.
    Authors: Ichii O, Konno A, Sasaki N, Endoh D, Hashimoto Y, Kon Y
    Kidney Int., 2008-05-07;74(3):339-47.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr


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Mouse Fc gamma  RIIB/CD32b Antibody
By Anonymous on 07/27/2016
Application: WB Sample Tested: Dendritic cells Species: Mouse