Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Co-Immunoprecipitation, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 190907
View all Fc gamma RIIB/CD32b Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
aa 40-217
Accession # P08101

Specificity

Detects mouse Fc gamma RIIB in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse (rm) Fc gamma RIII. In ELISAs, this antibody shows approximately 7% cross-reactivity with rmFc gamma  RIII.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Fc gamma  RIIB/CD32b Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Fc gamma  RIIB/CD32b (Catalog # 1460-CD)
under non-reducing conditions only

Mouse Fc gamma RIIA/CD32a Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse Fc gamma RII/RIII (CD32/CD16) Biotinylated Antibody (Catalog # BAF1460)
  • Standard: Recombinant Mouse Fc gamma RIIB/CD32b Protein, CF (Catalog # 1460-CD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB14601 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIIB/CD32b

Fc gamma RIIB, also known as CD32b, is a low affinity inhibitory receptor for the Fc portion of the IgG molecules. Fc gamma RIIB is expressed on B cells, macrophages, neutrophils, and mast cells and is involved in phagocytosis of immune complexes and modulation of antibody production by B cells.

Long Name

Fc gamma Receptor II

Alternate Names

CD32b, FCGR2B, FcgRIIB, FCRIIB

Entrez Gene IDs

2213 (Human); 14130 (Mouse); 289211 (Rat); 102139530 (Cynomolgus Monkey)

Gene Symbol

FCGR2B

UniProt

P08101

Additional Fc gamma RIIB/CD32b Products

Product Documents for Mouse Fc gamma  RIIB/CD32b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Fc gamma  RIIB/CD32b Antibody

For research use only

Citations for Mouse Fc gamma  RIIB/CD32b Antibody

Customer Reviews for Mouse Fc gamma  RIIB/CD32b Antibody (1)

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  • Mouse Fc gamma  RIIB/CD32b Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Dendritic cells
    Species: Mouse
    Verified Customer | Posted 07/27/2016

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