Mouse FoxC2 Antibody

Catalog #: AF6989
8 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6989
AF6989-SP

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Product Details

Citations (8)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse FoxC2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FoxC2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) FoxC2 is observed and less than 3% cross-reactivity with rhFoxF2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse FoxC2
Ala412-Tyr494
Accession # Q61850

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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FoxC2 in Mouse Embryo. FoxC2 was detected in immersion fixed frozen sections of mouse embryo (E15.5) using Sheep Anti-Mouse FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6989) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to periocular mesenchyme. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxC2

FOXC2 (Forkhead box protein C2; also BF-3, FKH-14 and MFH-1) is a 64-66 kDa member of the winged helix transcription factor gene family. It is widely expressed, including in endothelium where it induces the appearance of CXCR4 and integrin beta 3, two molecules essential to cell migration. High-calorie diets also induce FOXC2 in adipocytes, inducing a brown-fat like phenotype. Mouse FOXC2 is 494 amino acids (aa) in length. It contains a forkhead DNA binding domain (aa 71-148), a short poly-Arg segment (aa 162-166), one His-rich region (aa 386-395) and an Ala/Pro-rich domain (aa 396-415). There are seven potential Ser/Thr phosphorylation sites. Over aa 412-494, mouse FOXC2 shares 99% and 87% aa identity with rat and human FOXC2, respectively.

Long Name

Forkhead Box C2

Entrez Gene IDs

2303 (Human); 14234 (Mouse)

Alternate Names

Fkh14; FKHL14LD; forkhead box C2 (MFH-1, mesenchyme forkhead 1); forkhead, Drosophila, homolog-like 14; Forkhead-related protein FKHL14; FoxC2; LD; Mesenchyme fork head protein 1; mesenchyme forkhead 1; MFH-1 protein; MFH1; MFH-1; MFH1forkhead box protein C2; Transcription factor FKH-14

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse FoxC2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration
Authors: P Flora, MY Li, PM Galbo, M Astorkia, D Zheng, E Ezhkova
PloS Genetics, 2021-12-14;17(12):e1009948.
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC
DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors
Authors: MM Mikedis, Y Fan, PK Nicholls, T Endo, EK Jackson, SA Cobb, DG de Rooij, DC Page
Elife, 2020-07-20;9(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation
Authors: K Nonomura, V Lukacs, DT Sweet, LM Goddard, A Kanie, T Whitwam, SS Ranade, T Fujimori, ML Kahn, A Patapoutia
Proc. Natl. Acad. Sci. U.S.A., 2018-11-27;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Complementary Wnt Sources Regulate Lymphatic Vascular Development via PROX1-Dependent Wnt/?-Catenin Signaling
Authors: B Cha, X Geng, MR Mahamud, JY Zhang, L Chen, W Kim, EH Jho, Y Kim, D Choi, JB Dixon, H Chen, YK Hong, L Olson, TH Kim, BJ Merrill, MJ Davis, RS Srinivasan
Cell Rep, 2018-10-16;25(3):571-584.e5.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot
Apolipoprotein A-I Modulates Atherosclerosis Through Lymphatic Vessel-Dependent Mechanisms in Mice
Authors: A Milasan, G Jean, F Dallaire, JC Tardif, Y Merhi, M Sorci-Thom, C Martel
J Am Heart Assoc, 2017-09-22;6(9):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves
Authors: Boksik Cha
Genes Dev, 2016-06-16;30(12):1454-69.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-Fr, Western Blot
Lymph flow regulates collecting lymphatic vessel maturation in vivo.
Authors: Sweet D, Jimenez J, Chang J, Hess P, Mericko-Ishizuka P, Fu J, Xia L, Davies P, Kahn M
J Clin Invest, 2015-07-27;125(8):2995-3007.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
J Clin Invest, 2014-07-25;124(9):3960-74.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC