Mouse FoxC2 Antibody

Catalog # Availability Size / Price Qty
AF6989
AF6989-SP
Mouse FoxC2 Antibody in Data
1 Image
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews

Mouse FoxC2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FoxC2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) FoxC2 is observed and less than 3% cross-reactivity with rhFoxF2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse FoxC2
Ala412-Tyr494
Accession # Q61850
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry FoxC2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

FoxC2 in Mouse Embryo. FoxC2 was detected in immersion fixed frozen sections of mouse embryo (E15.5) using Sheep Anti-Mouse FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6989) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to periocular mesenchyme. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxC2

FOXC2 (Forkhead box protein C2; also BF-3, FKH-14 and MFH-1) is a 64-66 kDa member of the winged helix transcription factor gene family. It is widely expressed, including in endothelium where it induces the appearance of CXCR4 and integrin beta 3, two molecules essential to cell migration. High-calorie diets also induce FOXC2 in adipocytes, inducing a brown-fat like phenotype. Mouse FOXC2 is 494 amino acids (aa) in length. It contains a forkhead DNA binding domain (aa 71-148), a short poly-Arg segment (aa 162-166), one His-rich region (aa 386-395) and an Ala/Pro-rich domain (aa 396-415). There are seven potential Ser/Thr phosphorylation sites. Over aa 412-494, mouse FOXC2 shares 99% and 87% aa identity with rat and human FOXC2, respectively.

Long Name
Forkhead Box C2
Entrez Gene IDs
2303 (Human); 14234 (Mouse)
Alternate Names
Fkh14; FKHL14LD; forkhead box C2 (MFH-1, mesenchyme forkhead 1); forkhead, Drosophila, homolog-like 14; Forkhead-related protein FKHL14; FoxC2; LD; Mesenchyme fork head protein 1; mesenchyme forkhead 1; MFH-1 protein; MFH1; MFH-1; MFH1forkhead box protein C2; Transcription factor FKH-14

Product Datasheets

Citations for Mouse FoxC2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Filter by:

  1. DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors
    Authors: MM Mikedis, Y Fan, PK Nicholls, T Endo, EK Jackson, SA Cobb, DG de Rooij, DC Page
    Elife, 2020;9(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation
    Authors: K Nonomura, V Lukacs, DT Sweet, LM Goddard, A Kanie, T Whitwam, SS Ranade, T Fujimori, ML Kahn, A Patapoutia
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Complementary Wnt Sources Regulate Lymphatic Vascular Development via PROX1-Dependent Wnt/?-Catenin Signaling
    Authors: B Cha, X Geng, MR Mahamud, JY Zhang, L Chen, W Kim, EH Jho, Y Kim, D Choi, JB Dixon, H Chen, YK Hong, L Olson, TH Kim, BJ Merrill, MJ Davis, RS Srinivasan
    Cell Rep, 2018;25(3):571-584.e5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Apolipoprotein A-I Modulates Atherosclerosis Through Lymphatic Vessel-Dependent Mechanisms in Mice
    Authors: A Milasan, G Jean, F Dallaire, JC Tardif, Y Merhi, M Sorci-Thom, C Martel
    J Am Heart Assoc, 2017;6(9):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves
    Authors: Boksik Cha
    Genes Dev, 2016;30(12):1454-69.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Lymph flow regulates collecting lymphatic vessel maturation in vivo.
    Authors: Sweet D, Jimenez J, Chang J, Hess P, Mericko-Ishizuka P, Fu J, Xia L, Davies P, Kahn M
    J Clin Invest, 2015;125(8):2995-3007.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
    Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
    J Clin Invest, 2014;124(9):3960-74.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse FoxC2 Antibody

There are currently no reviews for this product. Be the first to review Mouse FoxC2 Antibody and earn rewards!

Have you used Mouse FoxC2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review