FPRP (F2 alpha Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F2 alpha receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton. Mature mouse FPRP is 858 amino acids (aa) in length. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa 22‑813) plus a Pro-Thr-Glu-Leu ER retention signal. The ECD of mouse FPRP (aa 22‑832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively.
Mouse FPRP/PTGFRN Antibody [Biotin]
R&D Systems | Catalog # BAF4495
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Flow Cytometry
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FPRP
Arg22-Pro832
Accession # NP_035327
Arg22-Pro832
Accession # NP_035327
Specificity
Detects mouse FPRP/PTGFRN in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse FPRP/PTGFRN Antibody [Biotin]
Detection of FPRP/PTGFRN in 3T3‑L1 Mouse Cell Line by Flow Cytometry.
3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Sheep Anti-Mouse FPRP/PTGFRN Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4495, filled histogram) or isotype control antibody (Catalog # BAF108), followed by Streptavidin-Allophycocyanin (Catalog # F0050).Applications for Mouse FPRP/PTGFRN Antibody [Biotin]
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line
Sample: 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse FPRP/PTGFRN
Sample: Recombinant Mouse FPRP/PTGFRN
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FPRP/PTGFRN
Long Name
Prostaglandin F2-alpha Receptor Regulatory Protein
Alternate Names
CD315, CD9P-1, PTGFRN
Gene Symbol
PTGFRN
UniProt
Additional FPRP/PTGFRN Products
Product Documents for Mouse FPRP/PTGFRN Antibody [Biotin]
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse FPRP/PTGFRN Antibody [Biotin]
For research use only
Related Research Areas
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Protocols
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