Mouse FPRP/PTGFRN Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF4495

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Detection of FPRP/PTGFRN in 3T3‑L1 Mouse Cell Line by Flow Cytometry.
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Mouse FPRP/PTGFRN Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FPRP in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FPRP
Arg22-Pro832
Accession # NP_035327
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse FPRP
Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of FPRP/PTGFRN antibody in 3T3-L1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of FPRP/PTGFRN in 3T3‑L1 Mouse Cell Line by Flow Cytometry. 3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Sheep Anti-Mouse FPRP/PTGFRN Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4495, filled histogram) or isotype control antibody (Catalog # BAF108), followed by Streptavidin-Allophycocyanin (Catalog # F0050).

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FPRP/PTGFRN

FPRP (F2 alpha Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F2 alpha  receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton. Mature mouse FPRP is 858 amino acids (aa) in length. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa 22‑813) plus a Pro-Thr-Glu-Leu ER retention signal. The ECD of mouse FPRP (aa 22‑832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively.

Long Name
Prostaglandin F2-alpha Receptor Regulatory Protein
Entrez Gene IDs
5738 (Human); 19221 (Mouse); 29602 (Rat)
Alternate Names
CD315; CD9P-1; FPRP; PTGFRN

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