Mouse G-CSF Quantikine ELISA Kit

(22 citations)
(2 Reviews)
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (25 uL), Serum (50 uL)
  • Sensitivity
    5 pg/mL
  • Assay Range
    14.1 - 900 pg/mL (Cell Culture Supernates, Serum)
  • Specificity
    Natural and recombinant mouse G-CSF
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse G-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse G-CSF in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse G-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse G-CSF accurately. Results obtained using natural mouse G-CSF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse G-CSF.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
Intra-Assay Precision Inter-Assay Precision
Standard Deviation2.38.8434.115.550.6


The recovery of mouse G-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 92 82-110
Serum (n=5) 96 80-119
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse G-CSF in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse G-CSF Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: G-CSF
G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.
    • Long Name
      Granulocyte Colony Stimulating Factor
    • Entrez Gene IDs
      1440 (Human); 12985 (Mouse);
    • Alternate Names
      C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 22
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    Sample Type
    1. SB203580 increases G-CSF production via a stem-loop destabilizing element in the 3' untranslated region in macrophages independently of its effect on p38 MAPK activity.
      Authors: Chang S, Li H, Huang Y, Tasi W, Chou Y, Lu S
      J Biomed Sci, 2016;23(1):3.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    2. gammadelta T Cells Are Required for M2 Macrophage Polarization and Resolution of Ozone-Induced Pulmonary Inflammation in Mice.
      Authors: Mathews J, Kasahara D, Ribeiro L, Wurmbrand A, Ninin F, Shore S
      PLoS ONE, 2015;10(7):e0131236.
      Species: Mouse
      Sample Type: BALF
    3. Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi.
      Authors: Brooks R, Iyer S, Akada H, Neelam S, Russo C, Chisholm J, Kerr W
      Stem Cells, 2015;33(3):848-58.
      Species: Mouse
      Sample Type: Serum
    4. SHIP1-expressing mesenchymal stem cells regulate hematopoietic stem cell homeostasis and lineage commitment during aging.
      Authors: Iyer S, Brooks R, Gumbleton M, Kerr W
      Stem Cells Dev, 2015;24(9):1073-81.
      Species: Mouse
      Sample Type: Serum
    5. Central role of conventional dendritic cells in regulation of bone marrow release and survival of neutrophils.
      Authors: Jiao, Jingjing, Dragomir, Ana-Cris, Kocabayoglu, Peri, Rahman, Adeeb H, Chow, Andrew, Hashimoto, Daigo, Leboeuf, Marylene, Kraus, Thomas, Moran, Thomas, Carrasco-Avino, Gonzalo, Friedman, Scott L, Merad, Miriam, Aloman, Costica
      J Immunol, 2014;192(7):3374-82.
      Species: Mouse
      Sample Type: Plasma
    6. Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis.
      Authors: Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, Kopf M, Manz M
      Blood, 2014;124(9):1393-403.
    7. Steady-state neutrophil homeostasis is dependent on TLR4/TRIF signaling.
      Authors: Bugl S, Wirths S, Radsak M, Schild H, Stein P, Andre M, Muller M, Malenke E, Wiesner T, Marklin M, Frick J, Handgretinger R, Rammensee H, Kanz L, Kopp H
      Blood, 2013;121(5):723-33.
      Species: Mouse
      Sample Type: Plasma
    8. Conditional TRF1 knockout in the hematopoietic compartment leads to bone marrow failure and recapitulates clinical features of dyskeratosis congenita.
      Authors: Beier, Fabian, Foronda, Miguel, Martinez, Paula, Blasco, Maria A
      Blood, 2012;120(15):2990-3000.
      Species: Mouse
      Sample Type: Serum
    9. Deletion of tristetraprolin caused spontaneous reactive granulopoiesis by a non-cell-autonomous mechanism without disturbing long-term hematopoietic stem cell quiescence.
      Authors: Kaplan IM, Morisot S, Heiser D, Cheng WC, Kim MJ, Civin CI
      J. Immunol., 2011;186(5):2826-34.
      Species: Mouse
      Sample Type: Plasma
    10. Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism.
      Authors: Waight JD, Hu Q, Miller A, Liu S, Abrams SI
      PLoS ONE, 2011;6(11):e27690.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    11. Bcl3 prevents acute inflammatory lung injury in mice by restraining emergency granulopoiesis.
      Authors: Kreisel D, Sugimoto S, Tietjens J, Zhu J, Yamamoto S, Krupnick AS, Carmody RJ, Gelman AE
      J. Clin. Invest., 2011;121(1):265-76.
      Species: Mouse
      Sample Type: Serum
    12. Severe congenital neutropenia resulting from G6PC3 deficiency with increased neutrophil CXCR4 expression and myelokathexis.
      Authors: McDermott DH, De Ravin SS, Jun HS
      Blood, 2010;116(15):2793-802.
      Species: Mouse
      Sample Type: Serum
    13. Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents.
      Authors: Shaked Y, Henke E, Roodhart JM, Mancuso P, Langenberg MH, Colleoni M, Daenen LG, Man S, Xu P, Emmenegger U, Tang T, Zhu Z, Witte L, Strieter RM, Bertolini F, Voest EE, Benezra R, Kerbel RS
      Cancer Cell, 2008;14(3):263-73.
      Species: Mouse
      Sample Type: Plasma
    14. The role of Rac2 in regulating neutrophil production in the bone marrow and circulating neutrophil counts.
      Authors: Gomez JC, Soltys J, Okano K, Dinauer MC, Doerschuk CM
      Am. J. Pathol., 2008;173(2):507-17.
      Species: Mouse
      Sample Type: Plasma
    15. Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
      Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
      Proc. Natl. Acad. Sci. U.S.A., 2007;104(43):17069-74.
      Species: Mouse
      Sample Type: Plasma
    16. Regulation of systemic and local neutrophil responses by G-CSF during pulmonary Pseudomonas aeruginosa infection.
      Authors: Gregory AD, Hogue LA, Ferkol TW, Link DC
      Blood, 2007;109(8):3235-43.
      Species: Mouse
      Sample Type: epithelial lining fluid
    17. Enalapril increases ischemia-induced endothelial progenitor cell mobilization through manipulation of the CD26 system.
      Authors: Wang CH, Verma S, Hsieh IC, Chen YJ, Kuo LT, Yang NI, Wang SY, Wu MY, Hsu CM, Cheng CW, Cherng WJ
      J. Mol. Cell. Cardiol., 2006;41(1):34-43.
      Species: Mouse
      Sample Type: Plasma
    18. Bone marrow-derived cells require a functional glucose 6-phosphate transporter for normal myeloid functions.
      Authors: Kim SY, Nguyen AD, Gao JL, Murphy PM, Mansfield BC, Chou JY
      J. Biol. Chem., 2006;281(39):28794-801.
      Species: Mouse
      Sample Type: Plasma
    19. A novel polysaccharide of black soybean promotes myelopoiesis and reconstitutes bone marrow after 5-flurouracil- and irradiation-induced myelosuppression.
      Authors: Liao HF, Chen YJ, Yang YC
      Life Sci., 2005;77(4):400-13.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    20. Granulocyte colony-stimulating factor: release is not impaired after burn wound infection.
      Authors: Gamelli R, He LK, Hahn E
      J Trauma, 2002;53(2):284-9; discus.
      Species: Mouse
      Sample Type: Serum
    21. Modulation of marrow proliferation and chemosensitivity by tumor-produced cytokines from syngeneic pancreatic tumor lines.
      Authors: Blumenthal RD, Reising A, Leon E
      Clin. Cancer Res., 2002;8(5):1301-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    22. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
      Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
      Arch. Dermatol. Res., 2001;293(10):508-14.
      Species: Mouse
      Sample Type: Cell Culture Supernates
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    Images Ratings Applications Species Reviewed By Date Details
     Mouse G-CSF Quantikine ELISA Kit MCS00
      Anonymous 06/17/2016
    Image Details
     Mouse G-CSF Quantikine ELISA Kit MCS00
    : Mouse G-CSF Quantikine ELISA Kit [MCS00]


    Sample TestedIMR-32 human neuroblastoma cell line
      Anonymous 06/10/2016


    Sample TestedIMR-32 human neuroblastoma cell line,SH-SY5Y human neuroblastoma cell line

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