Mouse G-CSF Quantikine ELISA Kit

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MCS00
Mouse G-CSF ELISA Standard Curve
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Product Details
Procedure
Citations (41)
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Reviews (6)

Mouse G-CSF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (50 uL)
Sensitivity
5 pg/mL
Assay Range
14.1 - 900 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse G-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse G-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse G-CSF in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse G-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse G-CSF accurately. Results obtained using natural mouse G-CSF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse G-CSF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 64.4 196 697 64.8 195 620
Standard Deviation 2.3 8.8 43 4.1 15.5 50.6
CV% 3.6 4.5 6.2 6.3 7.9 8.2

Recovery

The recovery of mouse G-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 92 82-110
Serum (n=5) 96 80-119

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse G-CSF in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse G-CSF ELISA Linearity

Data Examples

Mouse G-CSF ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: G-CSF

Mouse granulocyte-colony stimulating factor (G-CSF) is a 24-25 kDa monomeric glycoprotein that regulates the proliferation, differentiation and activation of hematopoietic cells. Mouse G-CSF cDNA encodes a 208 amino acid (aa) precursor protein with a 30 aa signal sequence that is proteolytically cleaved to form a 178 aa O-glycosylated mature protein containing two intrachain disulfide bridges. In humans, two distinct cDNA clones, encoding a 204 aa form and a minor alternatively spliced 207 aa form of G-CSF precursors, have been isolated. Mouse and human G-CSF are 76% identical at the aa sequence level and the two proteins show species cross-reactivity. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IL-1. Other cell types, including fibroblasts, endothelial cells, astrocytes and bone marrow stroma cells, can also secrete G-CSF after activation. In addition, various tumor cells express G-CSF constitutively.  


Mouse G-CSF receptor (G-CSF R) is a 120 kDa, type I transmembrane glycoprotein that belongs to the hematopoietin receptor superfamily. The mature protein is 812 amino acids in length and contains a 601 aa extracellular region, a 24 aa transmembrane segment, and a 187 aa cytoplasmic domain. The extracellular region contains multiple modules, including an N-terminal Ig-like domain, a cytokine receptor homology domain, and three fibronectin type III domains. Based on crystallographic study, G-CSF receptor forms a complex with the ligand in a 2:2 ratio. Mouse and human G-CSF receptor share 63% aa sequence identity. Cells known to express G-CSF R include monocytes and neutrophils, megakaryocytes and platelets, CD34+ CD33+ and CD34+ CD38+ hematopoietic progenitors, trophoblasts, endothelial cells and various tumor cell types.  

G-CSF is an important regulator for granulopoiesis in vivo. It has been demonstrated that G-CSF can support the growth of multi-lineage hematopoietic progenitor cells without influencing their commitment to the myeloid lineage and mobilize hematopoietic progenitor cells from the bone marrow into the bloodstream. On mature neutrophils, G-CSF may regulate neutrophil survival by controlling their rate of apoptosis. G-CSF has also been shown to enhance the functional capacity of mature neutrophils. As a consequence of its effects on hematopoietic progenitor cells, G-CSF has been shown to enhance monocytopoiesis in the presence M-CSF. Within the peripheral blood stem cell population mobilized with G-CSF, selective increases in the number of T helper 2-inducing dendritic cells are found.

Long Name:
Granulocyte Colony Stimulating Factor
Entrez Gene IDs:
1440 (Human); 12985 (Mouse)
Alternate Names:
C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; Pluripoietin
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse G-CSF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. G-CSF partially mediates effects of sleeve gastrectomy on the bone marrow niche
    Authors: Z Li, J Hardij, SS Evers, CR Hutch, SM Choi, Y Shao, BS Learman, KT Lewis, RL Schill, H Mori, DP Bagchi, SM Romanelli, KS Kim, E Bowers, C Griffin, RJ Seeley, K Singer, DA Sandoval, CJ Rosen, OA MacDougald
    J. Clin. Invest., 2019;129(6):2404-2416.
    Species: Mouse
    Sample Types: Serum
  2. Targeting myeloid-derived suppressor cells in combination with primary mammary tumor resection reduces metastatic growth in the lungs
    Authors: M Bosiljcic, RA Cederberg, MJ Hamilton, NE LePard, BT Harbourne, JL Collier, EC Halvorsen, R Shi, SE Franks, AY Kim, JP Banáth, M Hamer, FM Rossi, KL Bennewith
    Breast Cancer Res., 2019;21(1):103.
    Species: Mouse
    Sample Types: Serum
  3. Complement receptor C3aR1 controls neutrophil mobilization following spinal cord injury through physiological antagonism of CXCR2
    Authors: FH Brennan, T Jogia, ER Gillespie, LV Blomster, XX Li, B Nowlan, GM Williams, E Jacobson, GW Osborne, FA Meunier, SM Taylor, KE Campbell, KP MacDonald, JP Levesque, TM Woodruff, MJ Ruitenberg
    JCI Insight, 2019;4(9):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Pentosan Polysulfate Treatment of Mucopolysaccharidosis Type IIIA Mice
    Authors: N Guo, V DeAngelis, C Zhu, EH Schuchman, CM Simonaro
    JIMD Rep, 2018;0(0):.
    Species: Mouse
    Sample Types: Plasma
  5. Inhibition of Dpp8/9 Activates the Nlrp1b Inflammasome
    Authors: MC Okondo, SD Rao, CY Taabazuing, AJ Chui, SE Poplawski, DC Johnson, DA Bachovchin
    Cell Chem Biol, 2018;0(0):.
    Species: Mouse
    Sample Types: Serum
  6. Modulating bone marrow hematopoietic lineage potential to prevent bone metastasis in breast cancer
    Authors: JM Ubellacker, N Baryawno, N Severe, MJ DeCristo, J Sceneay, JN Hutchinson, MT Haider, CS Rhee, Y Qin, WM Gregory, AC Garrido-Ca, I Holen, JE Brown, RE Coleman, DT Scadden, SS McAllister
    Cancer Res., 2018;0(0):.
    Species: Mouse
    Sample Types: Plasma
  7. Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors
    Authors: M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos
    Mol. Cancer Ther., 2018;0(0):.
    Species: Mouse
    Sample Types: Plasma
  8. Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling
    Authors: W Lu, D Jia, S An, M Mu, X Qiao, Y Liu, X Li, D Wang
    Sci Rep, 2017;7(1):8402.
    Species: Mouse
    Sample Types: Plasma
  9. The Toll-Like Receptor 2/6 Agonist, FSL-1 Lipopeptide, Therapeutically Mitigates Acute Radiation Syndrome
    Authors: CJ Kurkjian, H Guo, ND Montgomery, N Cheng, H Yuan, JR Merrill, GD Sempowski, WJ Brickey, JP Ting
    Sci Rep, 2017;7(1):17355.
    Species: Mouse
    Sample Types: Serum
  10. Cannabinoid Receptor 2 Modulates Neutrophil Recruitment in a Murine Model of Endotoxemia
    Authors: TS Kapellos, C Recio, DR Greaves, AJ Iqbal
    Mediators Inflamm., 2017;2017(0):4315412.
    Species: Mouse
    Sample Types: Serum
  11. Peptidoglycan accelerates granulopoiesis through a TLR2- and MyD88-dependent pathway
    Authors: M Takehara, S Seike, T Takagishi, K Kobayashi, M Nagahama
    Biochem. Biophys. Res. Commun., 2017;487(2):419-425.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Neutrophils suppress intraluminal NK-mediated tumor cell clearance and enhance extravasation of disseminated carcinoma cells
    Authors: A Spiegel, MW Brooks, S Houshyar, F Reinhardt, M Ardolino, E Fessler, MB Chen, JA Krall, J DeCock, IK Zervantona, A Iannello, Y Iwamoto, V Cortez-Ret, RD Kamm, MJ Pittet, DH Raulet, RA Weinberg
    Cancer Discov, 2016;0(0):.
    Species: Mouse
    Sample Types: Plasma
  13. Growth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression
    Authors: Limo Chen
    Oncoimmunology, 2016;5(11):e1234570.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity
    Cell, 2016;0(0):.
    Species: Mouse
    Sample Types: Serum
  15. SB203580 increases G-CSF production via a stem-loop destabilizing element in the 3' untranslated region in macrophages independently of its effect on p38 MAPK activity.
    Authors: Chang S, Li H, Huang Y, Tasi W, Chou Y, Lu S
    J Biomed Sci, 2016;23(1):3.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Pten Cell Autonomously Modulates the Hematopoietic Stem Cell Response to Inflammatory Cytokines
    Stem Cell Reports, 2016;0(0):.
    Species: Mouse
    Sample Types: Serum
  17. Key role for neutrophils in radiation-induced antitumor immune responses: Potentiation with G-CSF
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. LPS-Induced G-CSF Expression in Macrophages Is Mediated by ERK2, but Not ERK1.
    Authors: Chang S, Lin S, Yang H, Chou Y, Gao J, Lu S
    PLoS ONE, 2015;10(6):e0129685.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. gammadelta T Cells Are Required for M2 Macrophage Polarization and Resolution of Ozone-Induced Pulmonary Inflammation in Mice.
    Authors: Mathews J, Kasahara D, Ribeiro L, Wurmbrand A, Ninin F, Shore S
    PLoS ONE, 2015;10(7):e0131236.
    Species: Mouse
    Sample Types: BALF
  20. Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi.
    Authors: Brooks R, Iyer S, Akada H, Neelam S, Russo C, Chisholm J, Kerr W
    Stem Cells, 2015;33(3):848-58.
    Species: Mouse
    Sample Types: Serum
  21. SHIP1-expressing mesenchymal stem cells regulate hematopoietic stem cell homeostasis and lineage commitment during aging.
    Authors: Iyer S, Brooks R, Gumbleton M, Kerr W
    Stem Cells Dev, 2015;24(9):1073-81.
    Species: Mouse
    Sample Types: Serum
  22. Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis.
    Authors: Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, Kopf M, Manz M
    Blood, 2014;124(9):1393-403.
  23. Erythropoietin guides multipotent hematopoietic progenitor cells toward an erythroid fate.
    Authors: Grover, Amit, Mancini, Elena, Moore, Susan, Mead, Adam J, Atkinson, Deborah, Rasmussen, Kasper D, O'Carroll, Donal, Jacobsen, Sten Eir, Nerlov, Claus
    J Exp Med, 2014;211(2):181-8.
    Species: Mouse
    Sample Types: Serum
  24. Central role of conventional dendritic cells in regulation of bone marrow release and survival of neutrophils.
    Authors: Jiao, Jingjing, Dragomir, Ana-Cris, Kocabayoglu, Peri, Rahman, Adeeb H, Chow, Andrew, Hashimoto, Daigo, Leboeuf, Marylene, Kraus, Thomas, Moran, Thomas, Carrasco-Avino, Gonzalo, Friedman, Scott L, Merad, Miriam, Aloman, Costica
    J Immunol, 2014;192(7):3374-82.
    Species: Mouse
    Sample Types: Plasma
  25. Steady-state neutrophil homeostasis is dependent on TLR4/TRIF signaling.
    Authors: Bugl S, Wirths S, Radsak M, Schild H, Stein P, Andre M, Muller M, Malenke E, Wiesner T, Marklin M, Frick J, Handgretinger R, Rammensee H, Kanz L, Kopp H
    Blood, 2013;121(5):723-33.
    Species: Mouse
    Sample Types: Plasma
  26. Protein malnutrition induces bone marrow mesenchymal stem cells commitment to adipogenic differentiation leading to hematopoietic failure.
    Authors: Cunha M, Lima F, Vinolo M, Hastreiter A, Curi R, Borelli P, Fock R
    PLoS ONE, 2013;8(3):e58872.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Conditional TRF1 knockout in the hematopoietic compartment leads to bone marrow failure and recapitulates clinical features of dyskeratosis congenita.
    Authors: Beier, Fabian, Foronda, Miguel, Martinez, Paula, Blasco, Maria A
    Blood, 2012;120(15):2990-3000.
    Species: Mouse
    Sample Types: Serum
  28. Deletion of tristetraprolin caused spontaneous reactive granulopoiesis by a non-cell-autonomous mechanism without disturbing long-term hematopoietic stem cell quiescence.
    Authors: Kaplan IM, Morisot S, Heiser D, Cheng WC, Kim MJ, Civin CI
    J. Immunol., 2011;186(5):2826-34.
    Species: Mouse
    Sample Types: Plasma
  29. Bcl3 prevents acute inflammatory lung injury in mice by restraining emergency granulopoiesis.
    Authors: Kreisel D, Sugimoto S, Tietjens J, Zhu J, Yamamoto S, Krupnick AS, Carmody RJ, Gelman AE
    J. Clin. Invest., 2011;121(1):265-76.
    Species: Mouse
    Sample Types: Serum
  30. Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism.
    Authors: Waight JD, Hu Q, Miller A, Liu S, Abrams SI
    PLoS ONE, 2011;6(11):e27690.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Severe congenital neutropenia resulting from G6PC3 deficiency with increased neutrophil CXCR4 expression and myelokathexis.
    Authors: McDermott DH, De Ravin SS, Jun HS
    Blood, 2010;116(15):2793-802.
    Species: Mouse
    Sample Types: Serum
  32. Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents.
    Authors: Shaked Y, Henke E, Roodhart JM, Mancuso P, Langenberg MH, Colleoni M, Daenen LG, Man S, Xu P, Emmenegger U, Tang T, Zhu Z, Witte L, Strieter RM, Bertolini F, Voest EE, Benezra R, Kerbel RS
    Cancer Cell, 2008;14(3):263-73.
    Species: Mouse
    Sample Types: Plasma
  33. The role of Rac2 in regulating neutrophil production in the bone marrow and circulating neutrophil counts.
    Authors: Gomez JC, Soltys J, Okano K, Dinauer MC, Doerschuk CM
    Am. J. Pathol., 2008;173(2):507-17.
    Species: Mouse
    Sample Types: Plasma
  34. Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
    Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(43):17069-74.
    Species: Mouse
    Sample Types: Plasma
  35. Regulation of systemic and local neutrophil responses by G-CSF during pulmonary Pseudomonas aeruginosa infection.
    Authors: Gregory AD, Hogue LA, Ferkol TW, Link DC
    Blood, 2007;109(8):3235-43.
    Species: Mouse
    Sample Types: Epithelial Fluid
  36. Bone marrow-derived cells require a functional glucose 6-phosphate transporter for normal myeloid functions.
    Authors: Kim SY, Nguyen AD, Gao JL, Murphy PM, Mansfield BC, Chou JY
    J. Biol. Chem., 2006;281(39):28794-801.
    Species: Mouse
    Sample Types: Plasma
  37. Enalapril increases ischemia-induced endothelial progenitor cell mobilization through manipulation of the CD26 system.
    Authors: Wang CH, Verma S, Hsieh IC, Chen YJ, Kuo LT, Yang NI, Wang SY, Wu MY, Hsu CM, Cheng CW, Cherng WJ
    J. Mol. Cell. Cardiol., 2006;41(1):34-43.
    Species: Mouse
    Sample Types: Plasma
  38. A novel polysaccharide of black soybean promotes myelopoiesis and reconstitutes bone marrow after 5-flurouracil- and irradiation-induced myelosuppression.
    Authors: Liao HF, Chen YJ, Yang YC
    Life Sci., 2005;77(4):400-13.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  39. Modulation of marrow proliferation and chemosensitivity by tumor-produced cytokines from syngeneic pancreatic tumor lines.
    Authors: Blumenthal RD, Reising A, Leon E
    Clin. Cancer Res., 2002;8(5):1301-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  40. Granulocyte colony-stimulating factor: release is not impaired after burn wound infection.
    Authors: Gamelli R, He LK, Hahn E
    J Trauma, 2002;53(2):284-9; discus.
    Species: Mouse
    Sample Types: Serum
  41. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
    Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
    Arch. Dermatol. Res., 2001;293(10):508-14.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Reviews for Mouse G-CSF Quantikine ELISA Kit

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Mouse G-CSF Quantikine ELISA Kit
By Anonymous on 07/13/2018
Application: Sample Tested: Serum

Mouse G-CSF Quantikine ELISA Kit
By Anonymous on 06/12/2018
Application: Sample Tested: Serum

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