Mouse G-CSF Quantikine ELISA Kit

  (35 citations)
(4 Reviews)
    
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Product Details
Assay Procedure
Citations (35)
FAQs
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (25 uL), Serum (50 uL)
  • Sensitivity
    5 pg/mL
  • Assay Range
    14.1 - 900 pg/mL (Cell Culture Supernates, Serum)
  • Specificity
    Natural and recombinant mouse G-CSF
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse G-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse G-CSF in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse G-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse G-CSF accurately. Results obtained using natural mouse G-CSF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse G-CSF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 64.4 196 697 64.8 195 620
Standard Deviation 2.3 8.8 43 4.1 15.5 50.6
CV% 3.6 4.5 6.2 6.3 7.9 8.2

Recovery

The recovery of mouse G-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 92 82-110
Serum (n=5) 96 80-119
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse G-CSF in each matrix were diluted with Calibrator Diluent and then assayed.
 G-CSF [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: G-CSF
G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.
  • Long Name:
    Granulocyte Colony Stimulating Factor
  • Entrez Gene IDs:
    1440 (Human); 12985 (Mouse)
  • Alternate Names:
    C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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Species
Sample Type
  1. Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors
    Authors: M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos
    Mol. Cancer Ther., 2018;0(0):.
    Species: Mouse
    Sample Type: Plasma
  2. Peptidoglycan accelerates granulopoiesis through a TLR2- and MyD88-dependent pathway
    Authors: M Takehara, S Seike, T Takagishi, K Kobayashi, M Nagahama
    Biochem. Biophys. Res. Commun., 2017;487(2):419-425.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  3. The Toll-Like Receptor 2/6 Agonist, FSL-1 Lipopeptide, Therapeutically Mitigates Acute Radiation Syndrome
    Authors: CJ Kurkjian, H Guo, ND Montgomery, N Cheng, H Yuan, JR Merrill, GD Sempowski, WJ Brickey, JP Ting
    Sci Rep, 2017;7(1):17355.
    Species: Mouse
    Sample Type: Serum
  4. Cannabinoid Receptor 2 Modulates Neutrophil Recruitment in a Murine Model of Endotoxemia
    Authors: TS Kapellos, C Recio, DR Greaves, AJ Iqbal
    Mediators Inflamm., 2017;2017(0):4315412.
    Species: Mouse
    Sample Type: Serum
  5. Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling
    Authors: W Lu, D Jia, S An, M Mu, X Qiao, Y Liu, X Li, D Wang
    Sci Rep, 2017;7(1):8402.
    Species: Mouse
    Sample Type: Plasma
  6. Growth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression
    Authors: Limo Chen
    Oncoimmunology, 2016;5(11):e1234570.
    Species: Mouse
    Sample Type: Tissue Homogenates
  7. Key role for neutrophils in radiation-induced antitumor immune responses: Potentiation with G-CSF
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
  8. Pten Cell Autonomously Modulates the Hematopoietic Stem Cell Response to Inflammatory Cytokines
    Stem Cell Reports, 2016;0(0):.
    Species: Mouse
    Sample Type: Serum
  9. Neutrophils suppress intraluminal NK-mediated tumor cell clearance and enhance extravasation of disseminated carcinoma cells
    Authors: A Spiegel, MW Brooks, S Houshyar, F Reinhardt, M Ardolino, E Fessler, MB Chen, JA Krall, J DeCock, IK Zervantona, A Iannello, Y Iwamoto, V Cortez-Ret, RD Kamm, MJ Pittet, DH Raulet, RA Weinberg
    Cancer Discov, 2016;0(0):.
    Species: Mouse
    Sample Type: Plasma
  10. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity
    Cell, 2016;0(0):.
    Species: Mouse
    Sample Type: Serum
  11. SB203580 increases G-CSF production via a stem-loop destabilizing element in the 3' untranslated region in macrophages independently of its effect on p38 MAPK activity.
    Authors: Chang S, Li H, Huang Y, Tasi W, Chou Y, Lu S
    J Biomed Sci, 2016;23(1):3.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  12. Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi.
    Authors: Brooks R, Iyer S, Akada H, Neelam S, Russo C, Chisholm J, Kerr W
    Stem Cells, 2015;33(3):848-58.
    Species: Mouse
    Sample Type: Serum
  13. LPS-Induced G-CSF Expression in Macrophages Is Mediated by ERK2, but Not ERK1.
    Authors: Chang S, Lin S, Yang H, Chou Y, Gao J, Lu S
    PLoS ONE, 2015;10(6):e0129685.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  14. gammadelta T Cells Are Required for M2 Macrophage Polarization and Resolution of Ozone-Induced Pulmonary Inflammation in Mice.
    Authors: Mathews J, Kasahara D, Ribeiro L, Wurmbrand A, Ninin F, Shore S
    PLoS ONE, 2015;10(7):e0131236.
    Species: Mouse
    Sample Type: BALF
  15. SHIP1-expressing mesenchymal stem cells regulate hematopoietic stem cell homeostasis and lineage commitment during aging.
    Authors: Iyer S, Brooks R, Gumbleton M, Kerr W
    Stem Cells Dev, 2015;24(9):1073-81.
    Species: Mouse
    Sample Type: Serum
  16. Erythropoietin guides multipotent hematopoietic progenitor cells toward an erythroid fate.
    Authors: Grover, Amit, Mancini, Elena, Moore, Susan, Mead, Adam J, Atkinson, Deborah, Rasmussen, Kasper D, O'Carroll, Donal, Jacobsen, Sten Eir, Nerlov, Claus
    J Exp Med, 2014;211(2):181-8.
    Species: Mouse
    Sample Type: Serum
  17. Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis.
    Authors: Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, Kopf M, Manz M
    Blood, 2014;124(9):1393-403.
  18. Central role of conventional dendritic cells in regulation of bone marrow release and survival of neutrophils.
    Authors: Jiao, Jingjing, Dragomir, Ana-Cris, Kocabayoglu, Peri, Rahman, Adeeb H, Chow, Andrew, Hashimoto, Daigo, Leboeuf, Marylene, Kraus, Thomas, Moran, Thomas, Carrasco-Avino, Gonzalo, Friedman, Scott L, Merad, Miriam, Aloman, Costica
    J Immunol, 2014;192(7):3374-82.
    Species: Mouse
    Sample Type: Plasma
  19. Steady-state neutrophil homeostasis is dependent on TLR4/TRIF signaling.
    Authors: Bugl S, Wirths S, Radsak M, Schild H, Stein P, Andre M, Muller M, Malenke E, Wiesner T, Marklin M, Frick J, Handgretinger R, Rammensee H, Kanz L, Kopp H
    Blood, 2013;121(5):723-33.
    Species: Mouse
    Sample Type: Plasma
  20. Protein malnutrition induces bone marrow mesenchymal stem cells commitment to adipogenic differentiation leading to hematopoietic failure.
    Authors: Cunha M, Lima F, Vinolo M, Hastreiter A, Curi R, Borelli P, Fock R
    PLoS ONE, 2013;8(3):e58872.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  21. Conditional TRF1 knockout in the hematopoietic compartment leads to bone marrow failure and recapitulates clinical features of dyskeratosis congenita.
    Authors: Beier, Fabian, Foronda, Miguel, Martinez, Paula, Blasco, Maria A
    Blood, 2012;120(15):2990-3000.
    Species: Mouse
    Sample Type: Serum
  22. Bcl3 prevents acute inflammatory lung injury in mice by restraining emergency granulopoiesis.
    Authors: Kreisel D, Sugimoto S, Tietjens J, Zhu J, Yamamoto S, Krupnick AS, Carmody RJ, Gelman AE
    J. Clin. Invest., 2011;121(1):265-76.
    Species: Mouse
    Sample Type: Serum
  23. Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism.
    Authors: Waight JD, Hu Q, Miller A, Liu S, Abrams SI
    PLoS ONE, 2011;6(11):e27690.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  24. Deletion of tristetraprolin caused spontaneous reactive granulopoiesis by a non-cell-autonomous mechanism without disturbing long-term hematopoietic stem cell quiescence.
    Authors: Kaplan IM, Morisot S, Heiser D, Cheng WC, Kim MJ, Civin CI
    J. Immunol., 2011;186(5):2826-34.
    Species: Mouse
    Sample Type: Plasma
  25. Severe congenital neutropenia resulting from G6PC3 deficiency with increased neutrophil CXCR4 expression and myelokathexis.
    Authors: McDermott DH, De Ravin SS, Jun HS
    Blood, 2010;116(15):2793-802.
    Species: Mouse
    Sample Type: Serum
  26. Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents.
    Authors: Shaked Y, Henke E, Roodhart JM, Mancuso P, Langenberg MH, Colleoni M, Daenen LG, Man S, Xu P, Emmenegger U, Tang T, Zhu Z, Witte L, Strieter RM, Bertolini F, Voest EE, Benezra R, Kerbel RS
    Cancer Cell, 2008;14(3):263-73.
    Species: Mouse
    Sample Type: Plasma
  27. The role of Rac2 in regulating neutrophil production in the bone marrow and circulating neutrophil counts.
    Authors: Gomez JC, Soltys J, Okano K, Dinauer MC, Doerschuk CM
    Am. J. Pathol., 2008;173(2):507-17.
    Species: Mouse
    Sample Type: Plasma
  28. Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
    Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(43):17069-74.
    Species: Mouse
    Sample Type: Plasma
  29. Regulation of systemic and local neutrophil responses by G-CSF during pulmonary Pseudomonas aeruginosa infection.
    Authors: Gregory AD, Hogue LA, Ferkol TW, Link DC
    Blood, 2007;109(8):3235-43.
    Species: Mouse
    Sample Type: Epithelial Fluid
  30. Bone marrow-derived cells require a functional glucose 6-phosphate transporter for normal myeloid functions.
    Authors: Kim SY, Nguyen AD, Gao JL, Murphy PM, Mansfield BC, Chou JY
    J. Biol. Chem., 2006;281(39):28794-801.
    Species: Mouse
    Sample Type: Plasma
  31. Enalapril increases ischemia-induced endothelial progenitor cell mobilization through manipulation of the CD26 system.
    Authors: Wang CH, Verma S, Hsieh IC, Chen YJ, Kuo LT, Yang NI, Wang SY, Wu MY, Hsu CM, Cheng CW, Cherng WJ
    J. Mol. Cell. Cardiol., 2006;41(1):34-43.
    Species: Mouse
    Sample Type: Plasma
  32. A novel polysaccharide of black soybean promotes myelopoiesis and reconstitutes bone marrow after 5-flurouracil- and irradiation-induced myelosuppression.
    Authors: Liao HF, Chen YJ, Yang YC
    Life Sci., 2005;77(4):400-13.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  33. Modulation of marrow proliferation and chemosensitivity by tumor-produced cytokines from syngeneic pancreatic tumor lines.
    Authors: Blumenthal RD, Reising A, Leon E
    Clin. Cancer Res., 2002;8(5):1301-9.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  34. Granulocyte colony-stimulating factor: release is not impaired after burn wound infection.
    Authors: Gamelli R, He LK, Hahn E
    J Trauma, 2002;53(2):284-9; discus.
    Species: Mouse
    Sample Type: Serum
  35. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
    Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
    Arch. Dermatol. Res., 2001;293(10):508-14.
    Species: Mouse
    Sample Type: Cell Culture Supernates
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 Mouse G-CSF Quantikine ELISA Kit MCS00 Array MCS00
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