Mouse Galectin-4 Antibody

Catalog # Availability Size / Price Qty
AF2128
AF2128-SP

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Detection of Galectin‑4 in Mouse Intestine.
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Product Details
Citations (4)
FAQs
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Reviews (3)

Mouse Galectin-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Galectin-4 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Galectin-4
Ala2-Ile326
Accession # Q8K419
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Galectin‑4 (Catalog # 2128-GA)
Immunohistochemistry
3-15 µg/mL
Immersion fixed paraffin-embedded sections of Mouse Intestine

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of Galectin‑4 in Mouse Intestine. Galectin‑4 was detected in immersion fixed paraffin-embedded sections of Mouse Intestine using Goat Anti-Mouse Galectin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2128) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in intestinal epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-4

Galectins are a family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins share structural similarities in their carbohydrate recognition domains (CRD), forming three groups often termed prototype (one CRD), tandem-repeat (two CRDs) and chimeric (one CRD, unique N-terminus) (1, 2). All lack classical signal peptides, but are present and active both within and outside of the cell. Galectins are involved in cell adhesion, migration, survival, and apoptosis, and are often up- or down-regulated in cancer (1-3). Galectin-4 is a 36 kDa tandem-repeat galectin found throughout the gastrointestinal tract, but also present in well-differentiated breast and liver carcinomas (3, 4). Each CRD binds a different set of carbohydrate groups, including those found on erythrocyte blood group antigens (3, 5). CRD1 also binds cholesterol 3-sulfate and other sulfatides, which are concentrated within lipid raft membrane microdomains (6, 7). Endocytosed Galectin-4 is thought to play a role in forming the rafts, delivering them to the intestinal apical membrane, and stabilizing highly detergent-resistant "superrafts" (7-9). Human Galectin-4 shares 76%, 77%, 78%, and 80% amino acid (aa) identity with mouse, rat, bovine, and porcine Galectin-4, respectively, with the highest identity occurring within the CRDs. A potential splice variant begins at aa 132 and lacks most of the first CRD (10). Galectin-4 expression is concentrated within microvilli in the gastrointestinal epithelium, where it can interact with CD3 and bind activated T cells in the lamina propria during intestinal inflammation (11, 12). Either pro- or anti-inflammatory activity has been shown, depending on the mouse model used. Galectin-4 can also bind lung, spleen, and kidney macrophages, although its expression is normally low in these tissues (5).

References
  1. Yang, R-Y. et al. (2008) Expert Rev. Mol. Med. 10:e17.
  2. Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
  3. Huflejt, M.E. and H. Leffler (2004) Glycoconj. J. 20:247.
  4. Recreche, H. et al. (1997) Eur. J. Biochem. 248:225.
  5. Markova, V. et al. (2006) Int. J. Mol. Med. 18:65.
  6. Ideo, H. et al. (2007) J. Biol. Chem. 282:21081.
  7. Delacour, D. et al. (2005) J. Cell Biol. 169:491.
  8. Braccia, A. et al. (2003) J. Biol. Chem. 278:15679.
  9. Stechly, L. et al. (2009) Traffic 10:438.
  10. Entrez accession # EAW56820.
  11. Hokama, A. et al. (2004) Immunity 20:681.
  12. Paclik, D. et al. (2008) PLoS ONE 3:e2629.
    Entrez Gene IDs
    3960 (Human); 16855 (Mouse)
    Alternate Names
    Antigen NY-CO-27; GAL4; gal-4; galectin 4; Galectin4; Galectin-4; L-36 lactose-binding protein; L36LBP; Lactose-binding lectin 4; lectin, galactoside-binding, soluble, 4; LGALS4

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    Citations for Mouse Galectin-4 Antibody

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    4 Citations: Showing 1 - 4
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    1. Reciprocal regulation of pancreatic ductal adenocarcinoma growth and molecular subtype by HNF4 alpha and SIX1/4
      Authors: Soledad A. Camolotto, Veronika K. Belova, Luke Torre-Healy, Jeffery M. Vahrenkamp, Kristofer C. Berrett, Hannah Conway et al.
      Gut
    2. An NKX2-1/ERK/WNT feedback loop modulates gastric identity and response to targeted therapy in lung adenocarcinoma
      Authors: Rediet Zewdu, Elnaz Mirzaei Mehrabad, Kelley Ingram, Pengshu Fang, Katherine L Gillis, Soledad A Camolotto et al.
      eLife
    3. FoxA1 and FoxA2 drive gastric differentiation and suppress squamous identity in NKX2-1-negative lung cancer
      Authors: Soledad A Camolotto, Shrivatsav Pattabiraman, Timothy L Mosbruger, Alex Jones, Veronika K Belova, Grace Orstad et al.
      eLife
    4. Brain glycolipids suppress T helper cells and inhibit autoimmune demyelination.
      Authors: Mycko M, Sliwinska B, Cichalewska M, Cwiklinska H, Raine C, Selmaj K
      J Neurosci, 2014-06-18;34(25):8646-58.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Neutralization

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    Reviews for Mouse Galectin-4 Antibody

    Average Rating: 4.7 (Based on 3 Reviews)

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    Mouse Galectin‑4 Antibody
    By Anonymous on 10/26/2015
    Application: Immunocytochemistry/Immunofluorescence Sample Tested: Cancer Cells Species: Human

    This antibody is as good as the monoclonal antibody from R&D.
    Specificity: Specific
    Sensitivity: Reasonably sensitive
    Buffer: PBS
    Dilution: 1:1000


    Mouse Galectin‑4 Antibody
    By Anonymous on 10/26/2015
    Application: Immunocytochemistry/Immunofluorescence Sample Tested: Colon tissue Species: Human

    Very specific, Red is propidium iodide the nuclear stain.
    Specificity: Specific
    Sensitivity: Reasonably sensitive
    Buffer: PBS
    Dilution: 1:500


    Mouse Galectin‑4 Antibody
    By Anonymous on 10/26/2015
    Application: 2D gel, protein modification Sample Tested: Cell Lysates Species: Human

    Although there were mutliple bands, the one circled are galectin-4, the others are interactors isolated on a 2D gel. Using human cell lysates in 2D buffer.
    Specificity: Specific
    Sensitivity: Sensitive
    Buffer: BSA
    Dilution: 1:1000