Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Galectin-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Galectin-4
Ala2-Ile326
Accession # Q8K419

Specificity

Detects mouse Galectin-4 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Galectin‑4 Antibody

Detection of Galectin‑4 in Mouse Intestine.

Galectin‑4 was detected in immersion fixed paraffin-embedded sections of Mouse Intestine using Goat Anti-Mouse Galectin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2128) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in intestinal epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse Galectin‑4 Antibody

Application
Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Mouse Intestine

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Galectin‑4 (Catalog # 2128-GA)

Reviewed Applications

Read 3 reviews rated 4.7 using AF2128 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-4

Galectins are a family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins share structural similarities in their carbohydrate recognition domains (CRD), forming three groups often termed prototype (one CRD), tandem-repeat (two CRDs) and chimeric (one CRD, unique N-terminus) (1, 2). All lack classical signal peptides, but are present and active both within and outside of the cell. Galectins are involved in cell adhesion, migration, survival, and apoptosis, and are often up- or down-regulated in cancer (1-3). Galectin-4 is a 36 kDa tandem-repeat galectin found throughout the gastrointestinal tract, but also present in well-differentiated breast and liver carcinomas (3, 4). Each CRD binds a different set of carbohydrate groups, including those found on erythrocyte blood group antigens (3, 5). CRD1 also binds cholesterol 3-sulfate and other sulfatides, which are concentrated within lipid raft membrane microdomains (6, 7). Endocytosed Galectin-4 is thought to play a role in forming the rafts, delivering them to the intestinal apical membrane, and stabilizing highly detergent-resistant "superrafts" (7-9). Human Galectin-4 shares 76%, 77%, 78%, and 80% amino acid (aa) identity with mouse, rat, bovine, and porcine Galectin-4, respectively, with the highest identity occurring within the CRDs. A potential splice variant begins at aa 132 and lacks most of the first CRD (10). Galectin-4 expression is concentrated within microvilli in the gastrointestinal epithelium, where it can interact with CD3 and bind activated T cells in the lamina propria during intestinal inflammation (11, 12). Either pro- or anti-inflammatory activity has been shown, depending on the mouse model used. Galectin-4 can also bind lung, spleen, and kidney macrophages, although its expression is normally low in these tissues (5).

References

  1. Yang, R-Y. et al. (2008) Expert Rev. Mol. Med. 10:e17.
  2. Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
  3. Huflejt, M.E. and H. Leffler (2004) Glycoconj. J. 20:247.
  4. Recreche, H. et al. (1997) Eur. J. Biochem. 248:225.
  5. Markova, V. et al. (2006) Int. J. Mol. Med. 18:65.
  6. Ideo, H. et al. (2007) J. Biol. Chem. 282:21081.
  7. Delacour, D. et al. (2005) J. Cell Biol. 169:491.
  8. Braccia, A. et al. (2003) J. Biol. Chem. 278:15679.
  9. Stechly, L. et al. (2009) Traffic 10:438.
  10. Entrez accession # EAW56820.
  11. Hokama, A. et al. (2004) Immunity 20:681.
  12. Paclik, D. et al. (2008) PLoS ONE 3:e2629.

    Alternate Names

    GAL4, Galectin4, LGALS4

    Entrez Gene IDs

    3960 (Human); 16855 (Mouse)

    Gene Symbol

    LGALS4

    UniProt

    Q8K419

    Additional Galectin-4 Products

    Product Documents for Mouse Galectin‑4 Antibody

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Note: Certificate of Analysis not available for kit components.

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    Product Specific Notices for Mouse Galectin‑4 Antibody

    For research use only

    Related Research Areas

    Lectins
    NKG2D Receptors and Ligands

    Citations for Mouse Galectin‑4 Antibody

    Customer Reviews for Mouse Galectin‑4 Antibody (3)

    4.7 out of 5
    3 Customer Ratings
    5 Stars
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    4 Stars
    33%
    3 Stars
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    Customer Images

    Showing  1 - 3 of 3 reviews Showing All
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    • Mouse Galectin‑4 Antibody
      Name: Anonymous
      Application: 2D gel, protein modification
      Sample Tested: Cell Lysates
      Species: Human
      Verified Customer | Posted 10/26/2015
      Although there were mutliple bands, the one circled are galectin-4, the others are interactors isolated on a 2D gel. Using human cell lysates in 2D buffer. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: BSA<br />Dilution: 1:1000
      Mouse Galectin‑4 Antibody AF2128
    • Mouse Galectin‑4 Antibody
      Name: Anonymous
      Application: Immunocytochemistry/Immunofluorescence
      Sample Tested: Cancer Cells
      Species: Human
      Verified Customer | Posted 10/26/2015
      This antibody is as good as the monoclonal antibody from R&D. <br />Specificity: Specific<br />Sensitivity: Reasonably sensitive<br />Buffer: PBS<br />Dilution: 1:1000
      Mouse Galectin‑4 Antibody AF2128
    • Mouse Galectin‑4 Antibody
      Name: Anonymous
      Application: Immunocytochemistry/Immunofluorescence
      Sample Tested: Colon tissue
      Species: Human
      Verified Customer | Posted 10/26/2015
      Very specific, Red is propidium iodide the nuclear stain. <br />Specificity: Specific<br />Sensitivity: Reasonably sensitive<br />Buffer: PBS<br />Dilution: 1:500
      Mouse Galectin‑4 Antibody AF2128

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