Mouse Galectin-7 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1304
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Mouse Galectin-7 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Galectin-7 in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross‑reactivity with recombinant human (rh) Galectin‑7 is observed and less than 0.5% cross-reactivity with rhGalectin-1, recombinant mouse Galectin-3, rhGalectin-4, and rhGalectin‑8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Galectin‑7
Ser2-Phe136
Accession # Q99ML7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Galectin‑7 (Catalog # 1304-GA)

Mouse Galectin-7 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Galectin‑7 Antibody (Catalog # MAB1304)

Standard: Recombinant Mouse Galectin-7 Protein (Catalog # 1304-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-7

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).

Mouse Galectin-7 is a prototype monomeric galectin. It is expressed in stratified epithelia and is significantly down-regulated in squamous cell carcinomas. Galectin-7 is a pro-apoptotic protein that is highly induced by the tumor suppressor protein p53. It functions intracellularly upstream of JNK activation to enhance cytochrome c release during apoptosis (5). Galectin-7 may also be involved in cell-cell and cell-matrix interactions and exogenous galectin has been found to accelerate the re‑epithelialization of wounds (6).

References
  1. Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2002) Biochimie 83:667.
  4. R&D Systems' Cytokine Bulletin, Summer (2002).
  5. Kuwabara, I. et al. (2002) J. Biol. Chem. 277:3487.
  6. Cao, Z. et al. (2002) J. Biol. Chem. 277:42299.
Entrez Gene IDs
3963 (Human); 16858 (Mouse)
Alternate Names
GAL7; Gal-7; galectin 7; Galectin7; Galectin-7; HKL-14; lectin, galactoside-binding, soluble, 7; LGALS7; LGALS7A; p53-induced gene 1 protein; PI7; PIG1; TP53I1

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