Mouse Gas1 Antibody

(4 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Gas1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human Gas1 and recombinant mouse Gas6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Gas1
    Leu39-Ser315
    Accession # Q01721
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed frozen sections of mouse embryo (13 d.p.c.)
    • Mouse Gas1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse Gas1 Antibody (Catalog # AF2644)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Gas1 Biotinylated Antibody (Catalog # BAF2644)
  • ELISA Standard
     
    Recombinant Mouse Gas1 Protein, CF (Catalog # 2644-GS)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Gas1 in Mouse Embryo. Gas1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Gas1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2644) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gas1

Gas1 (Growth Arrest Specific 1) is one of six structurally unrelated proteins that were identified by their increased expression in growth-arrested cells relative to actively proliferating cells (1, 2). Following mitogenic stimulation, Gas1 expression is transcriptionally suppressed by c-Myc as cells transit from G0 to G1 phases of the cell cycle (3, 4). Overexpression of Gas1 prevents S phase entry and DNA synthesis (5). Gas1-mediated blockade of the cell cycle is p53-dependent but does not require the transactivating domain of p53 (6). The mouse Gas1 cDNA encodes a 343 amino acid (aa) precursor that includes a 38 aa signal sequence, a 277 aa mature protein, and a 28 aa C-terminal propeptide. Gas1 contains Ala-rich and Asp-rich regions as well as an RGD sequence (5). Mature mouse and human Gas1 share 85% aa sequence identity. Mouse Gas1 is a 40 kDa GPI linked glycoprotein that is uniformly distributed on the cell surface (7). In contact inhibited vascular endothelial cells, Gas1 is induced by VE-Cadherin and VEGF expression and mediates the anti-apoptotic effect of VEGF (8). In contrast, Gas1 is induced in hippocampal neurons after NMDA exposure but functions as a pro-apoptotic effector of NMDA-mediated excitotoxicity (9). Gas1 exhibits a range of developmental effects including either promoting or inhibiting growth and differentiation of somite, limb, cerebellar, and eye tissues (10‑14). Gas1 mediates the antagonistic effect of Wnt proteins toward Shh function by binding the N-terminal region of Shh (11). The dependence of Gas1 functions on the cellular context has been addressed by suggesting that Gas1 could function as a co-receptor for GDNF family ligands (15). This speculation is supported by R&D Systems’ data that demonstrate direct binding of Gas1 to Artemin and Neurturin.

  • References:
    1. Schneider, C. et al. (1988) Cell 54:787.
    2. Mullor, J.L. and A.R. Altaba (2002) BioEssays 24:22.
    3. Del Sal, G. et al. (1994) Proc. Natl. Acad. Sci. USA 91:1848.
    4. Lee, T.C. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12886.
    5. Del Sal, G. et al. (1992) Cell 70:595.
    6. Del Sal, G. et al. (1995) Mol. Cell. Biol. 15:7152.
    7. Stebel, M. et al. (2000) FEBS Lett. 481:152.
    8. Spagnuolo, R. et al. (2004) Blood 103:3005.
    9. Mellstrom, B. et al. (2002) Mol. Cell Neurosci. 19:417.
    10. Lee, K.K.H. et al. (2001) Dev. Biol. 234:188.
    11. Lee, C.S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11347.
    12. Liu, Y. et al. (2002) Development 129:5289.
    13. Liu, Y. et al. (2001) Dev. Biol. 236:30.
    14. Lee, C.S. et al. (2001) Dev. Biol. 236:17.
    15. Schueler-Furman, O. et al. (2006) Trends Pharmacol. Sci. 27:72.
  • Long Name:
    Growth-arrest-specific Protein 1
  • Entrez Gene IDs:
    2619 (Human); 14451 (Mouse)
  • Alternate Names:
    Gas1; GAS-1; growth arrest-specific 1; Growth arrest-specific gene-1; growth arrest-specific protein 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Applications
Sample Type
  1. Gas1 is a receptor for sonic hedgehog to repel enteric axons.
    Authors: Jin S, Martinelli D, Zheng X, Tessier-Lavigne M, Fan C
    Proc Natl Acad Sci U S A, 2015;112(1):E73-80.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  2. Growth arrest-specific protein 1 is a novel endogenous inhibitor of glomerular cell activation and proliferation.
    Authors: van Roeyen C, Zok S, Pruessmeyer J, Boor P, Nagayama Y, Fleckenstein S, Cohen C, Eitner F, Grone H, Ostendorf T, Ludwig A, Floege J
    Kidney Int, 2012;83(2):251-63.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Epigenetic Transcriptional Regulation of the Growth Arrest-Specific gene 1 (Gas1) in Hepatic Cell Proliferation at Mononucleosomal Resolution.
    Authors: Sacilotto N, Espert A, Castillo J, Franco L, Lopez-Rodas G
    PLoS ONE, 2011;6(8):e23318.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Growth Arrest Specific 1 (Gas1) Gene Overexpression in Liver Reduces the In Vivo Progression of Murine Hepatocellular Carcinoma and Partially Restores Gene Expression Levels.
    Authors: Sacilotto N, Castillo J, Riffo-Campos A, Flores J, Hibbitt O, Wade-Martins R, Lopez C, Rodrigo M, Franco L, Lopez-Rodas G
    PLoS ONE, 0;10(7):e0132477.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 5 (Based on 2 reviews)

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We have 1 review tested in 1 species: Mouse.
We have 2 reviews tested in 2 applications: Immunoprecipitation, Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunoprecipitation Mouse Gas1 Antibody AF2644
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Excellent
 IP Anonymous 01/09/2017
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Immunoprecipitation Mouse Gas1 Antibody AF2644
Immunoprecipitation: Mouse Gas1 Antibody [AF2644]

Summary

ApplicationImmunoprecipitation
Sample TestedCELL CONDITIONED MEDIUM,EXTRACT CELL

Other Experimental Details

Other Experimental DetailsThis antibody also works perfectly for immunoprecipitation (IPP). In the article of Ayala-Sarmiento et al 2016, DOI:10.1007/s00418-016-1449-0, it is noticeable that this antibody is able to immunoprecipitate both the anchored and the soluble forms of Gas1. A clear and a single band is seen in the immunoprecipitation of the cell extract and two extracellular isoforms of soluble Gas1 are present in the conditioned medium. No signal is detected in the negative control of the IPP, see Figure.
  Excellent
 ICC/IF Mouse Anonymous 01/09/2016
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedEmbryonic neuronal tissue,Embryonic brain
SpeciesMouse

Other Experimental Details

Other Experimental DetailsIn the publication of Estudillo et al 2015: Gas1 is present in germinal niches of developing dentate gyrus and cortex. Cell and Tissue Research. It can be appreciated that the antibody gives a congruent and clear signal of GAS1 in embrionic and postnatal brain

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