Mouse Gas1 Antibody

Catalog # Availability Size / Price Qty
AF2644
AF2644-SP

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Gas1 in Mouse Embryo.
4 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (3)

Mouse Gas1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Gas1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human Gas1 and recombinant mouse Gas6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Gas1
Leu39-Ser315
Accession # Q01721
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Mouse Gas1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Gas1 Biotinylated Antibody (Catalog # BAF2644)

Standard: Recombinant Mouse Gas1 Protein, CF (Catalog # 2644-GS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Gas1 antibody in Mouse Embryo. View Larger

Gas1 in Mouse Embryo. Gas1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Gas1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2644) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Gas1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Gas1 by Immunocytochemistry/Immunofluorescence Immunofluorescent staining of E15.5 Wild type HoxAADD (a–d) and mutant Hoxaadd (Hoxa9,10,11−/−;Hoxd9,10,11−/−) (e–h) forelimbs. Arrowhead: radius, Arrow: ulna, the autopod is oriented to the left of the image. a and e: Six2 immunostaining, showing an increased expression in mutant chondrocytes. b and f: Gas1 staining, showing an increase in mutant limbs that is restricted to cells flanking chondrocytes, consistent with the inclusion of some perichondrial cells in the LCM samples. c and g: Lef1 staining, showing an absence of staining in mutant chondrocytes. d and h: Runx3 staining, showing an absence of staining in mutant chondrocytes Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26186931), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Gas1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Gas1 by Immunocytochemistry/Immunofluorescence Immunofluorescent staining of E15.5 Wild type HoxAADD (a–d) and mutant Hoxaadd (Hoxa9,10,11−/−;Hoxd9,10,11−/−) (e–h) forelimbs. Arrowhead: radius, Arrow: ulna, the autopod is oriented to the left of the image. a and e: Six2 immunostaining, showing an increased expression in mutant chondrocytes. b and f: Gas1 staining, showing an increase in mutant limbs that is restricted to cells flanking chondrocytes, consistent with the inclusion of some perichondrial cells in the LCM samples. c and g: Lef1 staining, showing an absence of staining in mutant chondrocytes. d and h: Runx3 staining, showing an absence of staining in mutant chondrocytes Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26186931), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Gas1 by Western Blot View Larger

Detection of Mouse Gas1 by Western Blot Transfection of Hepa 1–6 cells with the growth-arrest specific 1 (Gas1) gene.(A) Anti-HA immunofluorescence of Hepa 1–6 cells transfected with pcDNA3/CAG-HAGas1 in the absence of detergents to preserve the integrity of membranes. Nuclei were counterstained with DAPI. (B) Double immunofluorescent staining anti-HA/anti-BrdU of Hepa 1–6 cells transfected with pcDNA3/CAG-HAGas1. (C) Cell cycle analysis of GFP-positive HEPA 1–6 cells after transfection with pIRES/GFP empty vector. 106 cells (areas indicated in the upper row) were sorted and subjected to cell cycle analysis after propidium iodide staining (lower row). (D) As (C), after transfection with pIRES/HAGas1. (E) Quantitation of the % of cells in the different stages of the cell cycle from the flow cytometry analysis. Experiments were done in triplicate. (F) Analysis of Ccne2 expression in Hepa 1–6 cells overexpressing Gas1. A representative RT-PCR showing Gas1 and Ccne2 expression in cells transfected with either empty pcDNA3/CAG (control) or pcDNA3/CAG-HAGas1 (HAGas1), 15 h after transfection. (G) qRT-PCR to determine CycE2 expression in cells as in (F). qRT-PCR was performed in triplicate from three independent experiments. Values were averaged and normalized to 18S rRNA. **, p<0.01. ***, p<0.001. In A and B the bar represents 11 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26161998), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gas1

Gas1 (Growth Arrest Specific 1) is one of six structurally unrelated proteins that were identified by their increased expression in growth-arrested cells relative to actively proliferating cells (1, 2). Following mitogenic stimulation, Gas1 expression is transcriptionally suppressed by c-Myc as cells transit from G0 to G1 phases of the cell cycle (3, 4). Overexpression of Gas1 prevents S phase entry and DNA synthesis (5). Gas1-mediated blockade of the cell cycle is p53-dependent but does not require the transactivating domain of p53 (6). The mouse Gas1 cDNA encodes a 343 amino acid (aa) precursor that includes a 38 aa signal sequence, a 277 aa mature protein, and a 28 aa C-terminal propeptide. Gas1 contains Ala-rich and Asp-rich regions as well as an RGD sequence (5). Mature mouse and human Gas1 share 85% aa sequence identity. Mouse Gas1 is a 40 kDa GPI linked glycoprotein that is uniformly distributed on the cell surface (7). In contact inhibited vascular endothelial cells, Gas1 is induced by VE-Cadherin and VEGF expression and mediates the anti-apoptotic effect of VEGF (8). In contrast, Gas1 is induced in hippocampal neurons after NMDA exposure but functions as a pro-apoptotic effector of NMDA-mediated excitotoxicity (9). Gas1 exhibits a range of developmental effects including either promoting or inhibiting growth and differentiation of somite, limb, cerebellar, and eye tissues (10‑14). Gas1 mediates the antagonistic effect of Wnt proteins toward Shh function by binding the N-terminal region of Shh (11). The dependence of Gas1 functions on the cellular context has been addressed by suggesting that Gas1 could function as a co-receptor for GDNF family ligands (15). This speculation is supported by R&D Systems’ data that demonstrate direct binding of Gas1 to Artemin and Neurturin.

References
  1. Schneider, C. et al. (1988) Cell 54:787.
  2. Mullor, J.L. and A.R. Altaba (2002) BioEssays 24:22.
  3. Del Sal, G. et al. (1994) Proc. Natl. Acad. Sci. USA 91:1848.
  4. Lee, T.C. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12886.
  5. Del Sal, G. et al. (1992) Cell 70:595.
  6. Del Sal, G. et al. (1995) Mol. Cell. Biol. 15:7152.
  7. Stebel, M. et al. (2000) FEBS Lett. 481:152.
  8. Spagnuolo, R. et al. (2004) Blood 103:3005.
  9. Mellstrom, B. et al. (2002) Mol. Cell Neurosci. 19:417.
  10. Lee, K.K.H. et al. (2001) Dev. Biol. 234:188.
  11. Lee, C.S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11347.
  12. Liu, Y. et al. (2002) Development 129:5289.
  13. Liu, Y. et al. (2001) Dev. Biol. 236:30.
  14. Lee, C.S. et al. (2001) Dev. Biol. 236:17.
  15. Schueler-Furman, O. et al. (2006) Trends Pharmacol. Sci. 27:72.
Long Name
Growth-arrest-specific Protein 1
Entrez Gene IDs
2619 (Human); 14451 (Mouse)
Alternate Names
Gas1; GAS-1; growth arrest-specific 1; Growth arrest-specific gene-1; growth arrest-specific protein 1

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Citations for Mouse Gas1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Lhx2 is a progenitor-intrinsic modulator of Sonic Hedgehog signaling during early retinal neurogenesis
    Authors: X Li, PJ Gordon, JA Gaynes, AW Fuller, R Ringuette, CP Santiago, V Wallace, S Blackshaw, P Li, EM Levine
    Elife, 2022-12-02;11(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Growth Arrest Specific 1 (Gas1) Gene Overexpression in Liver Reduces the In Vivo Progression of Murine Hepatocellular Carcinoma and Partially Restores Gene Expression Levels.
    Authors: Sacilotto N, Castillo J, Riffo-Campos A, Flores J, Hibbitt O, Wade-Martins R, Lopez C, Rodrigo M, Franco L, Lopez-Rodas G
    PLoS ONE, 2015-07-10;10(7):e0132477.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Gas1 is a receptor for sonic hedgehog to repel enteric axons.
    Authors: Jin S, Martinelli D, Zheng X, Tessier-Lavigne M, Fan C
    Proc Natl Acad Sci U S A, 2014-12-22;112(1):E73-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Growth arrest-specific protein 1 is a novel endogenous inhibitor of glomerular cell activation and proliferation.
    Authors: van Roeyen C, Zok S, Pruessmeyer J, Boor P, Nagayama Y, Fleckenstein S, Cohen C, Eitner F, Grone H, Ostendorf T, Ludwig A, Floege J
    Kidney Int, 2012-12-19;83(2):251-63.
    Species: Human, Rat
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  5. Epigenetic Transcriptional Regulation of the Growth Arrest-Specific gene 1 (Gas1) in Hepatic Cell Proliferation at Mononucleosomal Resolution.
    Authors: Sacilotto N, Espert A, Castillo J, Franco L, Lopez-Rodas G
    PLoS ONE, 2011-08-09;6(8):e23318.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Mouse Gas1 Antibody
By Anonymous on 10/05/2021
Application: IHC Sample Tested: Melanoma tissue Species: Human

Mouse Gas1 Antibody
By Anonymous on 01/09/2017
Application: IP Sample Tested: CELL CONDITIONED MEDIUM,EXTRACT CELL Species: Mouse and Rat

This antibody also works perfectly for immunoprecipitation (IPP). In the article of Ayala-Sarmiento et al 2016, DOI:10.1007/s00418-016-1449-0, it is noticeable that this antibody is able to immunoprecipitate both the anchored and the soluble forms of Gas1. A clear and a single band is seen in the immunoprecipitation of the cell extract and two extracellular isoforms of soluble Gas1 are present in the conditioned medium. No signal is detected in the negative control of the IPP, see Figure.


Mouse Gas1 Antibody
By Anonymous on 01/09/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Embryonic brain,Embryonic neuronal tissue Species: Mouse

In the publication of Estudillo et al 2015: Gas1 is present in germinal niches of developing dentate gyrus and cortex. Cell and Tissue Research. It can be appreciated that the antibody gives a congruent and clear signal of GAS1 in embrionic and postnatal brain