Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Gas1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Gas1
Leu39-Ser315
Accession # Q01721

Specificity

Detects mouse Gas1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human Gas1 and recombinant mouse Gas6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Gas1 Antibody

Gas1 antibody in Mouse Embryo.

Gas1 in Mouse Embryo.

Gas1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Gas1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2644) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Gas1 by Immunocytochemistry/Immunofluorescence

Detection of Mouse Gas1 by Immunocytochemistry/Immunofluorescence

Immunofluorescent staining of E15.5 Wild type HoxAADD (a–d) and mutant Hoxaadd (Hoxa9,10,11−/−;Hoxd9,10,11−/−) (e–h) forelimbs. Arrowhead: radius, Arrow: ulna, the autopod is oriented to the left of the image. a and e: Six2 immunostaining, showing an increased expression in mutant chondrocytes. b and f: Gas1 staining, showing an increase in mutant limbs that is restricted to cells flanking chondrocytes, consistent with the inclusion of some perichondrial cells in the LCM samples. c and g: Lef1 staining, showing an absence of staining in mutant chondrocytes. d and h: Runx3 staining, showing an absence of staining in mutant chondrocytes Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26186931), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Gas1 by Immunocytochemistry/Immunofluorescence

Detection of Mouse Gas1 by Immunocytochemistry/Immunofluorescence

Immunofluorescent staining of E15.5 Wild type HoxAADD (a–d) and mutant Hoxaadd (Hoxa9,10,11−/−;Hoxd9,10,11−/−) (e–h) forelimbs. Arrowhead: radius, Arrow: ulna, the autopod is oriented to the left of the image. a and e: Six2 immunostaining, showing an increased expression in mutant chondrocytes. b and f: Gas1 staining, showing an increase in mutant limbs that is restricted to cells flanking chondrocytes, consistent with the inclusion of some perichondrial cells in the LCM samples. c and g: Lef1 staining, showing an absence of staining in mutant chondrocytes. d and h: Runx3 staining, showing an absence of staining in mutant chondrocytes Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26186931), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Gas1 by Western Blot

Detection of Mouse Gas1 by Western Blot

Transfection of Hepa 1–6 cells with the growth-arrest specific 1 (Gas1) gene.(A) Anti-HA immunofluorescence of Hepa 1–6 cells transfected with pcDNA3/CAG-HAGas1 in the absence of detergents to preserve the integrity of membranes. Nuclei were counterstained with DAPI. (B) Double immunofluorescent staining anti-HA/anti-BrdU of Hepa 1–6 cells transfected with pcDNA3/CAG-HAGas1. (C) Cell cycle analysis of GFP-positive HEPA 1–6 cells after transfection with pIRES/GFP empty vector. 106 cells (areas indicated in the upper row) were sorted and subjected to cell cycle analysis after propidium iodide staining (lower row). (D) As (C), after transfection with pIRES/HAGas1. (E) Quantitation of the % of cells in the different stages of the cell cycle from the flow cytometry analysis. Experiments were done in triplicate. (F) Analysis of Ccne2 expression in Hepa 1–6 cells overexpressing Gas1. A representative RT-PCR showing Gas1 and Ccne2 expression in cells transfected with either empty pcDNA3/CAG (control) or pcDNA3/CAG-HAGas1 (HAGas1), 15 h after transfection. (G) qRT-PCR to determine CycE2 expression in cells as in (F). qRT-PCR was performed in triplicate from three independent experiments. Values were averaged and normalized to 18S rRNA. **, p<0.01. ***, p<0.001. In A and B the bar represents 11 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26161998), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Gas1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Gas1 (Catalog # 2644-GS)

Mouse Gas1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse Gas1 Biotinylated Antibody (Catalog # BAF2644)
  • Standard: Recombinant Mouse Gas1 Protein, CF (Catalog # 2644-GS)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using AF2644 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Gas1

Gas1 (Growth Arrest Specific 1) is one of six structurally unrelated proteins that were identified by their increased expression in growth-arrested cells relative to actively proliferating cells (1, 2). Following mitogenic stimulation, Gas1 expression is transcriptionally suppressed by c-Myc as cells transit from G0 to G1 phases of the cell cycle (3, 4). Overexpression of Gas1 prevents S phase entry and DNA synthesis (5). Gas1-mediated blockade of the cell cycle is p53-dependent but does not require the transactivating domain of p53 (6). The mouse Gas1 cDNA encodes a 343 amino acid (aa) precursor that includes a 38 aa signal sequence, a 277 aa mature protein, and a 28 aa C-terminal propeptide. Gas1 contains Ala-rich and Asp-rich regions as well as an RGD sequence (5). Mature mouse and human Gas1 share 85% aa sequence identity. Mouse Gas1 is a 40 kDa GPI linked glycoprotein that is uniformly distributed on the cell surface (7). In contact inhibited vascular endothelial cells, Gas1 is induced by VE-Cadherin and VEGF expression and mediates the anti-apoptotic effect of VEGF (8). In contrast, Gas1 is induced in hippocampal neurons after NMDA exposure but functions as a pro-apoptotic effector of NMDA-mediated excitotoxicity (9). Gas1 exhibits a range of developmental effects including either promoting or inhibiting growth and differentiation of somite, limb, cerebellar, and eye tissues (10‑14). Gas1 mediates the antagonistic effect of Wnt proteins toward Shh function by binding the N-terminal region of Shh (11). The dependence of Gas1 functions on the cellular context has been addressed by suggesting that Gas1 could function as a co-receptor for GDNF family ligands (15). This speculation is supported by R&D Systems’ data that demonstrate direct binding of Gas1 to Artemin and Neurturin.

References

  1. Schneider, C. et al. (1988) Cell 54:787.
  2. Mullor, J.L. and A.R. Altaba (2002) BioEssays 24:22.
  3. Del Sal, G. et al. (1994) Proc. Natl. Acad. Sci. USA 91:1848.
  4. Lee, T.C. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12886.
  5. Del Sal, G. et al. (1992) Cell 70:595.
  6. Del Sal, G. et al. (1995) Mol. Cell. Biol. 15:7152.
  7. Stebel, M. et al. (2000) FEBS Lett. 481:152.
  8. Spagnuolo, R. et al. (2004) Blood 103:3005.
  9. Mellstrom, B. et al. (2002) Mol. Cell Neurosci. 19:417.
  10. Lee, K.K.H. et al. (2001) Dev. Biol. 234:188.
  11. Lee, C.S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11347.
  12. Liu, Y. et al. (2002) Development 129:5289.
  13. Liu, Y. et al. (2001) Dev. Biol. 236:30.
  14. Lee, C.S. et al. (2001) Dev. Biol. 236:17.
  15. Schueler-Furman, O. et al. (2006) Trends Pharmacol. Sci. 27:72.

Long Name

Growth-arrest-specific Protein 1

Alternate Names

GAS-1, growth arrest-specific 1, Growth arrest-specific gene-1, growth arrest-specific protein 1

Entrez Gene IDs

2619 (Human); 14451 (Mouse)

Gene Symbol

GAS1

UniProt

Q01721

Additional Gas1 Products

Product Documents for Mouse Gas1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Gas1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
GDNF Family

Citations for Mouse Gas1 Antibody

Customer Reviews for Mouse Gas1 Antibody (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse Gas1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • Mouse Gas1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Melanoma tissue
    Species: Human
    Verified Customer | Posted 10/05/2021
    Mouse Gas1 Antibody AF2644
  • Mouse Gas1 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: CELL CONDITIONED MEDIUM and EXTRACT CELL
    Species: Mouse and Rat
    Verified Customer | Posted 01/09/2017
    This antibody also works perfectly for immunoprecipitation (IPP). In the article of Ayala-Sarmiento et al 2016, DOI:10.1007/s00418-016-1449-0, it is noticeable that this antibody is able to immunoprecipitate both the anchored and the soluble forms of Gas1. A clear and a single band is seen in the immunoprecipitation of the cell extract and two extracellular isoforms of soluble Gas1 are present in the conditioned medium. No signal is detected in the negative control of the IPP, see Figure.
    Mouse Gas1 Antibody AF2644
  • Mouse Gas1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Embryonic neuronal tissue and Embryonic brain
    Species: Mouse
    Verified Customer | Posted 01/09/2016
    In the publication of Estudillo et al 2015: Gas1 is present in germinal niches of developing dentate gyrus and cortex. Cell and Tissue Research. It can be appreciated that the antibody gives a congruent and clear signal of GAS1 in embrionic and postnatal brain

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies