Mouse Gas6 Antibody

Name: Mouse Gas6 Antibody
Brand: R&D Systems
SKU: MAB986-SP
Rating: 5 (1 reviews)

Catalog #: MAB986
3 Citations 1 Review Datasheet / COA / SDS

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MAB986
MAB986-SP

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Product Details

Citations (3)

FAQs

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Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Gas6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Gas6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human Gas6.

Source

Monoclonal Rat IgG_2A Clone # 126618

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Gas6
Asp115-Pro674 (Del Pro530)
Accession # Q61592.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Gas6 (Catalog # 986-GS)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gas6

Growth arrest-specific gene 6 (Gas6) was initially characterized as a gene whose expression was up-regulated in serum starved NIH 3T3 fibroblasts and whose expression is down-regulated during growth induction. Mouse Gas6 is a 673 amino acid protein that shares 81% identity to its human homolog. Gas6 is a member of the vitamin K-dependent family of proteins that includes human protein S, a negative coregulator in the blood coagulation pathway. Gas6 and protein S share structural motifs that characterize this family: an extensively gamma -carboxylated amino terminus (Gla domain), four EGF-like repeats, and a carboxy terminus containing globular (G) domains with homology to steroid hormone-binding globulin. It is a ligand for the Axl (Ufo/Ark), Sky (Dtk/Tyro3/Rse/Brt/Tif), and Mer (Eyk) families of tyrosine kinase receptors. Gas6 binds to these receptors via tandem G domains at its C-terminus. Gas6 is ubiquitously expressed, but most abundantly in lung, intestine, bone marrow and endothelium. Gas6 has been implicated in a variety of biological processes. It serves as a mitogen for fibroblasts, endothelial cells, neural cells, vascular smooth muscle cells and several tumor-derived cell lines. In addition, Gas6 prevents apoptosis, independent of its mitogenic activity. Gas6 is capable of inducing cell adhesion and chemotaxis in specific cell types. It also supports vitamin K-independent hematopoiesis when expressed by stromal cells. Gas6 signal transduction following Axl/Sky/Mer receptor activation has been reported to occur through such diverse signaling pathways as PI3K, MAP kinase, Src, Ras and beta -catenin.

References

Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
Goruppi, S. et al. (1996) Oncogene 12:471.
Crosier, K and P. Crosier (1997) Pathology 29:131.
Dormady, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12260.
Goruppi, S. et al. (2001) Mol. Cell Biol. 21:902.

Long Name

Growth-arrest-specific Protein 6

Entrez Gene IDs

2621 (Human); 14456 (Mouse)

Alternate Names

AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Gas6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Microbiota-dependent and -independent postnatal development of salivary immunity
Authors: K Zubeidat, Y Jaber, Y Saba, O Barel, R Naamneh, Y Netanely, Y Horev, L Eli-Bercho, A Shhadeh, O Yosef, E Arbib, G Betser-Coh, C Nadler, H Shapiro, E Elinav, DJ Aframian, A Wilensky, AH Hovav
Cell Reports, 2023-01-07;42(1):111981.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Stromal changes in the aged lung induce an emergence from melanoma dormancy
Authors: ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna
Nature, 2022-06-01;606(7913):396-405.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Mer tyrosine kinase (MerTK) promotes macrophage survival following exposure to oxidative stress.
Authors: Anwar A, Keating AK, Joung D, Sather S, Kim GK, Sawczyn KK, Brandao L, Henson PM, Graham DK
J. Leukoc. Biol., 2009-04-22;86(1):73-9.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot