Mouse Gas6 Antibody Summary
Asp115-Pro674 (Del Pro530)
Accession # Q61592.2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Gas6 by Western Blot MRL MSC produced and released higher amount of MANF than BL6, BALB/c and DBA1 MSC. (A) Proteomic analysis of differential expression of MANF, GAS6, NENF, and SEMA5A in MRL MSC compared to BL6 MSC secretomes. Effect size indicate the standardized mean difference in protein expression level between MRL MSC and BL6 MSC. For each protein, the median intensity levels (Log 2 value) in MRL MSC and BL6 MSC are indicated. Normalized protein intensities were used to calculate the Effect size MRL/BL6. (B) Western blot analysis of MANF, GAS6, NENF, and SEMA5A in whole-cell extracts from BL6, BALB/c and DBA1 MSC. The intensity value of each target protein band was normalized against the intensity value of alpha -Tubulin gel band used as the internal loading control for each sample. (C)MANF mRNA expression level in MRL MSC and BL6 MSC. Western blot analysis of supernatants of BL6, BALB/c, and DBA1 MSC showing blotted proteins stained with Ponceau S (D) and the MANF protein band revealed by the anti-MANF antibody (E). MRL MSC whole cell extract was used as a positive control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33738280), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gas6
Growth arrest-specific gene 6 (Gas6) was initially characterized as a gene whose expression was up-regulated in serum starved NIH 3T3 fibroblasts and whose expression is down-regulated during growth induction. Mouse Gas6 is a 673 amino acid protein that shares 81% identity to its human homolog. Gas6 is a member of the vitamin K-dependent family of proteins that includes human protein S, a negative coregulator in the blood coagulation pathway. Gas6 and protein S share structural motifs that characterize this family: an extensively gamma -carboxylated amino terminus (Gla domain), four EGF-like repeats, and a carboxy terminus containing globular (G) domains with homology to steroid hormone-binding globulin. It is a ligand for the Axl (Ufo/Ark), Sky (Dtk/Tyro3/Rse/Brt/Tif), and Mer (Eyk) families of tyrosine kinase receptors. Gas6 binds to these receptors via tandem G domains at its C-terminus. Gas6 is ubiquitously expressed, but most abundantly in lung, intestine, bone marrow and endothelium. Gas6 has been implicated in a variety of biological processes. It serves as a mitogen for fibroblasts, endothelial cells, neural cells, vascular smooth muscle cells and several tumor-derived cell lines. In addition, Gas6 prevents apoptosis, independent of its mitogenic activity. Gas6 is capable of inducing cell adhesion and chemotaxis in specific cell types. It also supports vitamin K-independent hematopoiesis when expressed by stromal cells. Gas6 signal transduction following Axl/Sky/Mer receptor activation has been reported to occur through such diverse signaling pathways as PI3K, MAP kinase, Src, Ras and beta -catenin.
- Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
- Goruppi, S. et al. (1996) Oncogene 12:471.
- Crosier, K and P. Crosier (1997) Pathology 29:131.
- Dormady, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12260.
- Goruppi, S. et al. (2001) Mol. Cell Biol. 21:902.
Product Datasheets
Citations for Mouse Gas6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Microbiota-dependent and -independent postnatal development of salivary immunity
Authors: K Zubeidat, Y Jaber, Y Saba, O Barel, R Naamneh, Y Netanely, Y Horev, L Eli-Bercho, A Shhadeh, O Yosef, E Arbib, G Betser-Coh, C Nadler, H Shapiro, E Elinav, DJ Aframian, A Wilensky, AH Hovav
Cell Reports, 2023-01-07;42(1):111981.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Stromal changes in the aged lung induce an emergence from melanoma dormancy
Authors: ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna
Nature, 2022-06-01;606(7913):396-405.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
MANF Produced by MRL Mouse-Derived Mesenchymal Stem Cells Is Pro-regenerative and Protects From Osteoarthritis
Authors: Gautier Tejedor, Patricia Luz-Crawford, Audrey Barthelaix, Karine Toupet, Sébastien Roudières, François Autelitano et al.
Frontiers in Cell and Developmental Biology
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Mer tyrosine kinase (MerTK) promotes macrophage survival following exposure to oxidative stress.
Authors: Anwar A, Keating AK, Joung D, Sather S, Kim GK, Sawczyn KK, Brandao L, Henson PM, Graham DK
J. Leukoc. Biol., 2009-04-22;86(1):73-9.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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