Mouse GDF-15 Antibody

Catalog # Availability Size / Price Qty
AF6385
AF6385-SP
GDF‑15 in Mouse Brain.
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Product Details
Citations (3)
FAQs
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Reviews (1)

Mouse GDF-15 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GDF-15 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GDF-15
Ser189-Ala303
Accession # Q9Z0J7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry GDF-15 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

GDF‑15 in Mouse Brain. GDF-15 was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglion) using Sheep Anti-Mouse GDF-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6385) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-15

GDF-15 (Growth/differentiation factor 15; also PTGF, PDF, PL-74 and NAG-1) is a 25-30 kDa homodimeric member of the TGF-beta superfamily of proteins. In rodent, GDF-15 is expressed in a diverse population of activated cell types, including hepatocytes, macrophages, Schwann cells, cardiomyocytes, osteoblasts, adipocytes, and epithelium of the small intestine and mammary gland. Functionally, GDF-15 has multiple effects, some tissue specific, including the induction of ACRP30 secretion from fat, an attenuation of the adverse effects of cardiac hypertrophy, and serving as a trophic factor for motor and sensory neurons. Mouse GDF-15 is synthesized as a 273 amino acid (aa) proprecursor that contains a cleavable 26 kDa, 158 aa glycosylated prodomain (aa 31-188) and a 12-14 kDa, 115 aa mature region (aa 189-303). In human, certain cells are noted to secrete an uncleaved 40 kDa proprecursor that, as a disulfide-linked homodimer, would run at about 80 kDa in nonreducing SDS-PAGE. Over aa 189-303, mouse GDF-15 shares 97% and 67% aa identity with rat and human GDF-15, respectively.

Long Name
Growth Differentiation Factor 15
Entrez Gene IDs
9518 (Human); 23886 (Mouse); 29455 (Rat)
Alternate Names
GDF15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC-1NSAID-activated gene 1 protein; MIC1Prostate differentiation factor; NAG-1; NAG-1NSAID-regulated gene 1 protein; NSAID (nonsteroidal inflammatory drug)-activated protein 1; PDF; PDFGDF-15; PLAB; PLABNRG-1; Placental bone morphogenetic protein; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta

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Citations for Mouse GDF-15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Deadenylase-dependent mRNA decay of GDF15 and FGF21 orchestrates food intake and energy expenditure
    Authors: Sakie Katsumura, Nadeem Siddiqui, Michael Rock Goldsmith, Jaime H. Cheah, Teppei Fujikawa, Genki Minegishi et al.
    Cell Metabolism
  2. Targeting AURKA in treatment of peritoneal tumor dissemination in gastrointestinal cancer
    Authors: H Ozawa, H Imazeki, Y Ogiwara, H Kawakubo, K Fukuda, Y Kitagawa, C Kudo-Saito
    Translational Oncology, 2021-12-10;16(0):101307.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Fc-GDF15 glyco-engineering and receptor binding affinity optimization for body weight regulation
    Authors: E Fung, L Kang, D Sapashnik, S Benard, A Sievers, Y Liu, G Yan, J Zhou, L Rodriguez, W Ma, WR Stochaj, E LaVallie, L Wroblewska, K Kelleher, A Tam, O Bezy, D Breen, JR Chabot, T He, L Lin, Z Wu, L Mosyak
    Scientific Reports, 2021-04-26;11(1):8921.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Capture

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Mouse GDF-15 Antibody
By Anonymous on 07/08/2020
Application: ELISA Sample Tested: Recombinant protein Species: Human