Mouse GDF-15 Antibody

R&D Systems | Catalog # AF6385

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Flow Cytometry, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all GDF-15 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse GDF-15
Ser189-Ala303
Accession # Q9Z0J7

Specificity

Detects mouse GDF-15 in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse GDF-15 Antibody

GDF-15 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

GDF‑15 in Mouse Brain.

GDF-15 was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglion) using Sheep Anti-Mouse GDF-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6385) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse GDF-15 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (trigeminal ganglion)

Reviewed Applications

Read 1 review rated 5 using AF6385 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDF-15

GDF-15 (Growth/differentiation factor 15; also PTGF, PDF, PL-74 and NAG-1) is a 25-30 kDa homodimeric member of the TGF-beta superfamily of proteins. In rodent, GDF-15 is expressed in a diverse population of activated cell types, including hepatocytes, macrophages, Schwann cells, cardiomyocytes, osteoblasts, adipocytes, and epithelium of the small intestine and mammary gland. Functionally, GDF-15 has multiple effects, some tissue specific, including the induction of ACRP30 secretion from fat, an attenuation of the adverse effects of cardiac hypertrophy, and serving as a trophic factor for motor and sensory neurons. Mouse GDF-15 is synthesized as a 273 amino acid (aa) proprecursor that contains a cleavable 26 kDa, 158 aa glycosylated prodomain (aa 31-188) and a 12-14 kDa, 115 aa mature region (aa 189-303). In human, certain cells are noted to secrete an uncleaved 40 kDa proprecursor that, as a disulfide-linked homodimer, would run at about 80 kDa in nonreducing SDS-PAGE. Over aa 189-303, mouse GDF-15 shares 97% and 67% aa identity with rat and human GDF-15, respectively.

Long Name

Growth Differentiation Factor 15

Alternate Names

GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta

Entrez Gene IDs

9518 (Human); 23886 (Mouse); 29455 (Rat)

Gene Symbol

GDF15

UniProt

Q9Z0J7

Additional GDF-15 Products

Product Documents for Mouse GDF-15 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse GDF-15 Antibody

For research use only

Citations for Mouse GDF-15 Antibody

Customer Reviews for Mouse GDF-15 Antibody (1)

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  • Mouse GDF-15 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 07/08/2020
    Mouse GDF-15 Antibody AF6385

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