Mouse GDF-8/Myostatin Propeptide Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects GDF‑8/Myostatin Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with mature recombinant mouse (rm) GDF-8 is observed and less than 5% cross-reactivity with mature rmGDF-1, -3, -5, -6, -7, -9, and recombinant human GDF-11 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse GDF‑8/Myostatin Propeptide
    Asn25-Ser265
    Accession # O08689
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse GDF‑8/Myostatin Propeptide (Catalog # 1539-PG)
  • Neutralization
    Measured by its ability to neutralize GDF‑8 Propeptide inhibition of GDF‑8/Myostatin-dependent hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 800 ng/mL Recombinant Mouse GDF‑8 Propeptide and 40 ng/mL Recombinant Mouse GDF‑8/Myostatin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
GDF‑8 Propeptide Inhibition of GDF‑8/Myostatin-induced Hemoglobin Expression and Neutralization by Mouse GDF‑8 Propeptide Antibody. Recombinant Mouse GDF‑8 Propeptide (Catalog # 1539‑PG) inhibits Recombinant Mouse GDF‑8/Myostatin (Catalog # 788‑G8) induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Inhibition of Recombinant Mouse GDF‑8/Myostatin (40 ng/mL) activity elicited by Recombinant Mouse GDF‑8 Propeptide (800 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse GDF‑8/Myostatin Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1539). The ND50 is typically 10-30 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GDF-8/Myostatin
Growth Differentiation Factor 8 (GDF-8), also known as Myostatin, is a secreted TGF-beta superfamily protein that is expressed specifically in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass (1‑3). Mouse GDF-8 is synthesized as a 376 amino acid (aa) preproprotein that consists of a 24 aa signal peptide, a 243 aa propeptide, and a 109 aa mature protein (2). Within the propeptide, mouse GDF-8 shares 96% and 99% aa sequence identity with human and rat GDF-8, respectively. GDF-8 is secreted as a preproprotein that is cleaved by BMP-1 family proteases to separate the 35‑40 kDa propeptide from the 12 kDa bioactive mature protein (4‑6). This results in a latent complex containing a disulfide-linked dimer of the mature protein and two noncovalently-associated molecules of the propeptide (2, 6). The GDF-8 propeptide functions as an inhibitor of mature GDF-8, and GDF-8 activity can also be inhibited through association with Follistatin, FLRG, Decorin, or GASP-1 (6‑11). The uncleaved GDF-8 proprotein binds Latent TGF-beta bp3 which can sequester it in the extracellular matrix and prevent the proteolytic cleavage of the propeptide (12). GDF-8 binds to the type II Activin receptor Activin RIIB which then associates with the type I receptors Activin RIB/ALK-4 or TGF-beta RI/ALK-5 to induce signaling (13). GDF-8 additionally inhibits adipogenic differentiation of mesenchymal stem cells and preadipocytes (14). Genetic deletion of GDF-8 or in vivo administration of the GDF-8 propeptide induces muscle hypertrophy as well as enhanced glucose utilization and insulin sensitivity and a reduction in overall fat mass (15, 16).
  • References:
    1. McPherron, A.C. (2010) Immunol. Endocr. Metab. Agents Med. Chem. 10:217.
    2. McPherron, A.C. et al. (1997) Nature 387:83.
    3. Zimmers, T.A. et al. (2002) Science 296:1486.
    4. Wolfman, N.M. et al. (2003) Proc. Natl. Acad. Sci. 100:15842.
    5. McFarlane, C. et al. (2005) Dev. Biol. 283:58.
    6. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. 98:9306.
    7. Thies, R.S. et al. (2001) Growth Factors 18:251.
    8. Amthor, H. et al. (2004) Dev. Biol. 270:19.
    9. Hill, J.J. et al. (2002) J. Biol. Chem. 277:40735.
    10. Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
    11. Hill, J.J. et al. (2003) Molecular Endocrinology 17:1144.
    12. Anderson, S.B. et al. (2008) J. Biol. Chem. 283:7027.
    13. Rebbapragada, A. et al. (2003) Mol. Cell. Biol. 23:7230.
    14. Guo, W. et al. (2008) J. Biol. Chem. 283:9136.
    15. Matsakas, A. et al. (2009) Neuromuscul. Disord. 19:489.
    16. Guo, T. et al. (2009) PloS ONE 4:e4937.
  • Long Name:
    Growth Differentiation Factor 8
  • Entrez Gene IDs:
    2660 (Human); 17700 (Mouse)
  • Alternate Names:
    GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSTN; Myostatin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Myostatin blockade with a fully human monoclonal antibody induces muscle hypertrophy and reverses muscle atrophy in young and aged mice.
    Authors: Latres E, Pangilinan J, Miloscio L, Bauerlein R, Na E, Potocky T, Huang Y, Eckersdorff M, Rafique A, Mastaitis J, Lin C, Murphy A, Yancopoulos G, Gromada J, Stitt T
    Skelet Muscle, 2015;5(0):34.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  2. A satellite cell-specific knockout of the androgen receptor reveals myostatin as a direct androgen target in skeletal muscle.
    Authors: Dubois V, Laurent M, Sinnesael M, Cielen N, Helsen C, Clinckemalie L, Spans L, Gayan-Ramirez G, Deldicque L, Hespel P, Carmeliet G, Vanderschueren D, Claessens F
    FASEB J, 2014;28(7):2979-94.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Myostatin expression, lymphocyte population, and potential cytokine production correlate with predisposition to high-fat diet induced obesity in mice.
    Authors: Lyons JA, Haring JS, Biga PR
    PLoS ONE, 2010;5(9):e12928.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
    Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
    J. Biol. Chem., 2008;283(20):13874-88.
    Species: Human
    Sample Type: Recombinant Protein
    Application: WB
  5. Muscular atrophy of caveolin-3-deficient mice is rescued by myostatin inhibition.
    Authors: Ohsawa Y, Hagiwara H, Nakatani M, Yasue A, Moriyama K, Murakami T, Tsuchida K, Noji S, Sunada Y
    J. Clin. Invest., 2006;116(11):2924-34.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IP
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