Mouse GFR alpha -like Antibody

Catalog # Availability Size / Price Qty
MAB5728-100
MAB5728-SP

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GFR alpha ‑like in HEK293 Human Cell Line Transfected.
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Mouse GFR alpha -like Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GFR alpha ‑like in direct ELISAs.
Source
Monoclonal Rat IgG1 Clone # 1045708
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line CHO-derived mouse GFR alpha ‑like
Gln20-Gly349
Accession # Q6SJE0-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
Immersion fixed HEK293 human embryonic kidney cell line transfected

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

GFR alpha ‑like in HEK293 Human Cell Line Transfected. GFR alpha ‑like was detected in immersion fixed HEK293 human embryonic kidney cell line transfected (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Rat Anti-Mouse GFR alpha ‑like Monoclonal Antibody (Catalog # MAB5728) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-like

GFR alpha-like (GDNF receptor-alpha-like) is a 25 kDa dimer and distant member of the GDNFR family of proteins (1). It is expressed on both fetal and adult hindbrain neurons of the CNS (2), and would appear to function as an anti-apoptotic molecule during neuronal stress. GFR alpha-like is a funcation receptor of GDF15 and implicated in diet-based obesity and insulin resistance through c-Ret signaling (2 - 4). Mature mouse GFR alpha-like is a 374 amino acid (aa) type I transmembrane protein. It contains a 330 aa extracellular region that shows a Gas1 domain (aa 220‑316), and a 23 aa cytoplasmic tail. Over aa 19‑353, mouse GFR alpha-like shares 72% and 71% aa identity with human and rat GFR alpha–like, respectively.

References

  1. Li, Z. et al. (2005) J. Neurochem. 95:361.
  2. Yang, L. et al. (2017) Nature Medicine 23:1158.
  3. Mullican, S. et al. (2017) Nature medicine 23:1150.
  4. Emmerson, P. et al. (2017) Nature Medicine 23:1215.

Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha-like
Entrez Gene IDs
389400 (Human); 404194 (Mouse); 501023 (Rat)
Alternate Names
GFR alpha like; GFR alpha-like; GFRAL; GRAL

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