Mouse GM-CSF Antibody

Catalog # Availability Size / Price Qty
MAB415-SP
MAB415-100
MAB415-500

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Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody.
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Citations (16)
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Mouse GM-CSF Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GM-CSF in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human GM‑CSF.
Source
Monoclonal Rat IgG2A Clone # MP122E9
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Yeast derived recombinant mouse GM-CSF
Accession # P01587
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse GM-CSF (Catalog # 415-ML)
under non-reducing conditions only

Mouse GM-CSF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse GM‑CSF Biotinylated Antibody (Catalog # BAF415)

Standard: Recombinant Mouse GM-CSF Protein (Catalog # 415-ML)

Neutralization
Measured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. Ihle, J.N. et al. (1984) Advances in Viral Oncology. In G. Klein (eds): Raven Press, New York, NY. 4:95. The Neutralization Dose (ND50) is typically 0.03-0.09 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse GM‑CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody. View Larger

Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody. Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND50 is typically 0.03-0.09 µg/mL.

In vivo assay Detection of Mouse GM-CSF by In vivo assay View Larger

Detection of Mouse GM-CSF by In vivo assay Effects of TRX on inflammatory cytokines in BALF from exposed to cigarette smoke and challenged with poly(I:C).(A) KC and (B) GM-CSF in BALF obtained 6 h and 3 days after poly(I:C) challenge. (C) Messenger RNA of GM-CSF in lung homogenates 3 days after poly(I:C) challenge in mice exposed to cigarette smoke treated with or without TRX. (D) Neutrophil and lymphocyte counts in BALF 3 days after poly(I:C) challenge in mice exposed to cigarette smoke treated with and without TRX or anti-GM-CSF antibody. Error bars represent standard deviation (SD) (A, B, and C, n = 3–4 per group; D and E, n = 5 per group); *p<0.05. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0079016), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GM-CSF

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8-10). Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells (14, 15).

References
  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
  5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
  6. Heuser, M. et al. (2007) Semin. Hematol. 44:148.
  7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
  8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
  9. Diederichs, K. et al. (1991) Science 254:1779.
  10. Gough, N.M. et al. (1984) Nature 309:763.
  11. Onetto-Pothier, N. et al. (1990) Blood 75:59.
  12. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9655.
  13. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  14. Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095.
  15. Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381.
Long Name
Granulocyte Macrophage Growth Factor
Entrez Gene IDs
1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Alternate Names
colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim

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Citations for Mouse GM-CSF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Genetically defined syngeneic mouse models of ovarian cancer as tools for the discovery of combination immunotherapy.
    Authors: Sonia Iyer, Shuang Zhang, Simge Yucel, Heiko Horn, Sean G. Smith, Ferenc Reinhardt et al.
    Cancer Discovery
  2. A pipeline for identification and validation of tumor-specific antigens in a mouse model of metastatic breast cancer
    Authors: Christa I. DeVette, Harika Gundlapalli, Shu-Chin Alicia Lai, Curtis P. McMurtrey, Ashley R. Hoover, Hem R. Gurung et al.
    OncoImmunology
  3. FGL2 promotes tumor progression in the CNS by suppressing CD103+ dendritic cell differentiation
    Authors: J Yan, Q Zhao, K Gabrusiewi, LY Kong, X Xia, J Wang, M Ott, J Xu, RE Davis, L Huo, G Rao, SC Sun, SS Watowich, AB Heimberger, S Li
    Nat Commun, 2019-01-25;10(1):448.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Neutrophils instruct homeostatic and pathological states in naive tissues
    Authors: Maria Casanova-Acebes, José A. Nicolás-Ávila, Jackson LiangYao Li, Susana García-Silva, Akhila Balachander, Andrea Rubio-Ponce et al.
    Journal of Experimental Medicine
  5. The Magnitude and IgG Subclass of Antibodies Elicited by Targeted DNA Vaccines Are Influenced by Specificity for APC Surface Molecules
    Authors: Ranveig Braathen, Heidi C. L. Spång, Mona M. Lindeberg, Even Fossum, Gunnveig Grødeland, Agnete B. Fredriksen et al.
    ImmunoHorizons
  6. Targeting GM-CSF in epithelial and vascular remodeling in experimental Eosinophilic Esophagitis
    Authors: Eóin N McNamee
    Allergy, 2017-01-23;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor
    Nat Med, 2016-05-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
    Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
    J Biol Chem, 2015-07-16;290(37):22338-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Thioredoxin-1 protects against neutrophilic inflammation and emphysema progression in a mouse model of chronic obstructive pulmonary disease exacerbation.
    Authors: Tanabe, Naoya, Hoshino, Yuma, Marumo, Satoshi, Kiyokawa, Hirofumi, Sato, Susumu, Kinose, Daisuke, Uno, Kazuko, Muro, Shigeo, Hirai, Toyohiro, Yodoi, Junji, Mishima, Michiaki
    PLoS ONE, 2013-11-11;8(11):e79016.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Properties of bcr-abl-transformed mouse 12B1 cells secreting interleukin-2 and granulocyte-macrophage colony stimulating factor (GM-CSF): II. Adverse effects of GM-CSF.
    Authors: Petrackova M, Stanek L, Mandys V, Dundr P, Vonka V
    Int. J. Oncol., 2012-03-22;40(6):1915-22.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  12. Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
    Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH
    J. Clin. Invest., 2011-10-17;121(11):4446-61.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
    Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM
    Blood, 2007-10-10;111(1):42-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. Recombinant Leishmania major secreting biologically active granulocyte-macrophage colony-stimulating factor survives poorly in macrophages in vitro and delays disease development in mice.
    Authors: Dumas C, Muyombwe A, Roy G, Matte C, Ouellette M, Olivier M, Papadopoulou B
    Infect. Immun., 2003-11-01;71(11):6499-509.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  15. Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes.
    Authors: Pechova J, Stĕpánová G, Kovar L, Kopecky J
    Folia Parasitol., 2002-01-01;49(2):153-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  16. Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen.
    Authors: Tomaki M, Zhao LL, Sjostrand M, Linden A, Ichinose M, Lotvall J
    Pulm Pharmacol Ther, 2002-01-01;15(2):161-8.
    Species: Mouse
    Sample Types:
    Applications: Neutralization

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Mouse GM-CSF Antibody
By Anonymous on 09/30/2021
Application: WB Sample Tested: DA3 mouse myeloma cell line Species: Mouse