Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry, ELISA Development, In vivo assay, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # MP122E9
View all GM-CSF Primary Antibodies »

Product Specifications

Immunogen

Yeast derived recombinant mouse GM-CSF
Accession # P01587

Specificity

Detects mouse GM-CSF in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human GM‑CSF.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse GM‑CSF Antibody

Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody.

Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody.

Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND50 is typically 0.03-0.09 µg/mL.
Detection of Mouse GM-CSF by In vivo assay

Detection of Mouse GM-CSF by In vivo assay

Effects of TRX on inflammatory cytokines in BALF from exposed to cigarette smoke and challenged with poly(I:C).(A) KC and (B) GM-CSF in BALF obtained 6 h and 3 days after poly(I:C) challenge. (C) Messenger RNA of GM-CSF in lung homogenates 3 days after poly(I:C) challenge in mice exposed to cigarette smoke treated with or without TRX. (D) Neutrophil and lymphocyte counts in BALF 3 days after poly(I:C) challenge in mice exposed to cigarette smoke treated with and without TRX or anti-GM-CSF antibody. Error bars represent standard deviation (SD) (A, B, and C, n = 3–4 per group; D and E, n = 5 per group); *p<0.05. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0079016), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse GM‑CSF Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse GM-CSF (Catalog # 415-ML)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. Ihle, J.N. et al. (1984) Advances in Viral Oncology. In G. Klein (eds): Raven Press, New York, NY. 4:95. The Neutralization Dose (ND50) is typically 0.03-0.09 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse GM‑CSF.

Mouse GM-CSF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse GM‑CSF Biotinylated Antibody (Catalog # BAF415)
  • Standard: Recombinant Mouse GM-CSF Protein (Catalog # 415-ML)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB415 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GM-CSF

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8-10). Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells (14, 15).

References

  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
  5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
  6. Heuser, M. et al. (2007) Semin. Hematol. 44:148.
  7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
  8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
  9. Diederichs, K. et al. (1991) Science 254:1779.
  10. Gough, N.M. et al. (1984) Nature 309:763.
  11. Onetto-Pothier, N. et al. (1990) Blood 75:59.
  12. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9655.
  13. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  14. Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095.
  15. Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Entrez Gene IDs

1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)

Gene Symbol

CSF2

UniProt

P01587

Additional GM-CSF Products

Product Documents for Mouse GM‑CSF Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse GM‑CSF Antibody

For research use only

Citations for Mouse GM‑CSF Antibody

Customer Reviews for Mouse GM‑CSF Antibody (1)

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  • Mouse GM-CSF Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: DA3 mouse myeloma cell line
    Species: Mouse
    Verified Customer | Posted 09/30/2021
    Mouse GM‑CSF Antibody MAB415

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