Mouse GM-CSF Antibody

(10 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse GM-CSF in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human GM‑CSF.
  • Source
    Monoclonal Rat IgG2A Clone # MP122E9
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Yeast derived recombinant mouse GM-CSF
    Accession # P01587
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse GM-CSF (Catalog # 415-ML)
    under non-reducing conditions only
    • Mouse GM-CSF Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse GM‑CSF Antibody (Catalog # MAB415)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse GM‑CSF Biotinylated Antibody (Catalog # BAF415)
  • ELISA Standard
     
    Recombinant Mouse GM-CSF Protein (Catalog # 415-ML)
  • Neutralization
    Measured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. Ihle, J.N. et al. (1984) Advances in Viral Oncology. In G. Klein (eds): Raven Press, New York, NY. 4:95. The Neutralization Dose (ND50) is typically 0.03-0.09 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse GM‑CSF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody. Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND50 is typically 0.03-0.09 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GM-CSF

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8-10). Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells (14, 15).

  • References:
    1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
    2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
    3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
    4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
    5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
    6. Heuser, M. et al. (2007) Semin. Hematol. 44:148.
    7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
    8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
    9. Diederichs, K. et al. (1991) Science 254:1779.
    10. Gough, N.M. et al. (1984) Nature 309:763.
    11. Onetto-Pothier, N. et al. (1990) Blood 75:59.
    12. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9655.
    13. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
    14. Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095.
    15. Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381.
  • Long Name:
    Granulocyte Macrophage Growth Factor
  • Entrez Gene IDs:
    1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
  • Alternate Names:
    colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; molgramostin; sargramostim
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor
    Nat Med, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
    Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
    J Biol Chem, 2015;290(37):22338-51.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. Thioredoxin-1 protects against neutrophilic inflammation and emphysema progression in a mouse model of chronic obstructive pulmonary disease exacerbation.
    Authors: Tanabe, Naoya, Hoshino, Yuma, Marumo, Satoshi, Kiyokawa, Hirofumi, Sato, Susumu, Kinose, Daisuke, Uno, Kazuko, Muro, Shigeo, Hirai, Toyohiro, Yodoi, Junji, Mishima, Michiaki
    PLoS ONE, 2013;8(11):e79016.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  4. Properties of bcr-abl-transformed mouse 12B1 cells secreting interleukin-2 and granulocyte-macrophage colony stimulating factor (GM-CSF): II. Adverse effects of GM-CSF.
    Authors: Petrackova M, Stanek L, Mandys V, Dundr P, Vonka V
    Int. J. Oncol., 2012;40(6):1915-22.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  5. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  6. Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
    Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH
    J. Clin. Invest., 2011;121(11):4446-61.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  7. The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
    Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM
    Blood, 2007;111(1):42-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  8. Recombinant Leishmania major secreting biologically active granulocyte-macrophage colony-stimulating factor survives poorly in macrophages in vitro and delays disease development in mice.
    Authors: Dumas C, Muyombwe A, Roy G, Matte C, Ouellette M, Olivier M, Papadopoulou B
    Infect. Immun., 2003;71(11):6499-509.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  9. Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes.
    Authors: Pechova J, Stĕpánová G, Kovar L, Kopecky J
    Folia Parasitol., 2002;49(2):153-9.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  10. Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen.
    Authors: Tomaki M, Zhao LL, Sjostrand M, Linden A, Ichinose M, Lotvall J
    Pulm Pharmacol Ther, 2002;15(2):161-8.
    Species: Mouse
    Sample Type:
    Application: In vivo
Isotype Controls
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Rat IgG2A Isotype Control

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Normal Rat IgG Control

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Rat IgG2A Isotype Control

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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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