Mouse GM-CSF Antibody
Mouse GM-CSF Antibody Summary
Accession # P01587
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Applications
Mouse GM-CSF Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody. Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND50 is typically 0.03-0.09 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GM-CSF
GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8-10). Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells (14, 15).
- Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
- Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
- Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
- Cao, Y. (2007) J. Clin. Invest. 117:2362.
- Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
- Heuser, M. et al. (2007) Semin. Hematol. 44:148.
- Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
- Kaushansky, K. et al. (1992) Biochemistry 31:1881.
- Diederichs, K. et al. (1991) Science 254:1779.
- Gough, N.M. et al. (1984) Nature 309:763.
- Onetto-Pothier, N. et al. (1990) Blood 75:59.
- Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9655.
- Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
- Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095.
- Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381.
Product Datasheets
Citations for Mouse GM-CSF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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FGL2 promotes tumor progression in the CNS by suppressing CD103+ dendritic cell differentiation
Authors: J Yan, Q Zhao, K Gabrusiewi, LY Kong, X Xia, J Wang, M Ott, J Xu, RE Davis, L Huo, G Rao, SC Sun, SS Watowich, AB Heimberger, S Li
Nat Commun, 2019;10(1):448.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor
Nat Med, 2016;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Targeting GM-CSF in epithelial and vascular remodeling in experimental Eosinophilic Esophagitis
Authors: Eóin N McNamee
Allergy, 2016;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
J Biol Chem, 2015;290(37):22338-51.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Thioredoxin-1 protects against neutrophilic inflammation and emphysema progression in a mouse model of chronic obstructive pulmonary disease exacerbation.
Authors: Tanabe, Naoya, Hoshino, Yuma, Marumo, Satoshi, Kiyokawa, Hirofumi, Sato, Susumu, Kinose, Daisuke, Uno, Kazuko, Muro, Shigeo, Hirai, Toyohiro, Yodoi, Junji, Mishima, Michiaki
PLoS ONE, 2013;8(11):e79016.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Properties of bcr-abl-transformed mouse 12B1 cells secreting interleukin-2 and granulocyte-macrophage colony stimulating factor (GM-CSF): II. Adverse effects of GM-CSF.
Authors: Petrackova M, Stanek L, Mandys V, Dundr P, Vonka V
Int. J. Oncol., 2012;40(6):1915-22.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development -
Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH
J. Clin. Invest., 2011;121(11):4446-61.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM
Blood, 2007;111(1):42-9.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Recombinant Leishmania major secreting biologically active granulocyte-macrophage colony-stimulating factor survives poorly in macrophages in vitro and delays disease development in mice.
Authors: Dumas C, Muyombwe A, Roy G, Matte C, Ouellette M, Olivier M, Papadopoulou B
Infect. Immun., 2003;71(11):6499-509.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen.
Authors: Tomaki M, Zhao LL, Sjostrand M, Linden A, Ichinose M, Lotvall J
Pulm Pharmacol Ther, 2002;15(2):161-8.
Species: Mouse
Sample Types:
Applications: Neutralization -
Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes.
Authors: Pechova J, Stĕpánová G, Kovar L, Kopecky J
Folia Parasitol., 2002;49(2):153-9.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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