Mouse GM-CSF Quantikine ELISA Kit

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MGM00
PMGM00
SMGM00
Mouse GM-CSF ELISA Standard Curve
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Product Details
Procedure
Citations (27)
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Mouse GM-CSF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Sensitivity
5.8 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse GM-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse GM-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse GM-CSF in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse GM-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse GM-CSF accurately. Results obtained using natural mouse GM-CSF showed dose-response curves that were parallel to the standard curves obtained using the recombinant standards. These results indicate that this kit can be used to determine relative mass values for natural mouse GM-CSF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 18.5 67.2 219 20 71.7 243
Standard Deviation 0.7 2.1 6.9 1 3.8 11.2
CV% 3.8 3.1 3.2 5 5.3 4.6

Recovery

The recovery of mouse GM-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=8) 96 82-114
Serum (n=7) 94 82-112

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse GM-CSF in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse GM-CSF ELISA Linearity

Data Examples

Mouse GM-CSF ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GM-CSF

GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.

Long Name:
Granulocyte Macrophage Growth Factor
Entrez Gene IDs:
1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Alternate Names:
colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse GM-CSF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. FGL2 promotes tumor progression in the CNS by suppressing CD103+ dendritic cell differentiation
    Authors: J Yan, Q Zhao, K Gabrusiewi, LY Kong, X Xia, J Wang, M Ott, J Xu, RE Davis, L Huo, G Rao, SC Sun, SS Watowich, AB Heimberger, S Li
    Nat Commun, 2019;10(1):448.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Arming oncolytic reovirus with GM-CSF gene to enhance immunity
    Authors: V Kemp, DJM van den Wo, MGM Camps, T van Hall, P Kinderman, N Pronk-van, RC Hoeben
    Cancer Gene Ther., 2018;0(0):.
  3. Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling
    Authors: W Lu, D Jia, S An, M Mu, X Qiao, Y Liu, X Li, D Wang
    Sci Rep, 2017;7(1):8402.
    Species: Mouse
    Sample Types: Plasma
  4. A heart-brain-kidney network controls adaptation to cardiac stress through tissue macrophage activation
    Authors: K Fujiu, M Shibata, Y Nakayama, F Ogata, S Matsumoto, K Noshita, S Iwami, S Nakae, I Komuro, R Nagai, I Manabe
    Nat. Med., 2017;23(5):611-622.
    Species: Mouse
    Sample Types: Serum
  5. Modifying Antigen-Encapsulating Liposomes with KALA Facilitates MHC Class I Antigen Presentation and Enhances Anti-tumor Effects
    Authors: N Miura, H Akita, N Tateshita, T Nakamura, H Harashima
    Mol. Ther, 2017;0(0):.
    Species: Mouse
    Sample Types: Serum
  6. Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression
    Authors: F Reggiani, V Labanca, P Mancuso, C Rabascio, G Talarico, S Orecchioni, A Manconi, F Bertolini
    Cancer Res., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Development and Preclinical Application of an Immunocompetent Transplant Model of Basal Breast Cancer with Lung, Liver and Brain Metastases
    PLoS ONE, 2016;11(5):e0155262.
    Species: Mouse
    Sample Types:
  8. Key role of microRNA in the regulation of granulocyte macrophage colony-stimulating factor expression in murine alveolar epithelial cells during oxidative stress.
    Authors: Sturrock, Anne, Mir-Kasimov, Mustafa, Baker, Jessica, Rowley, Jesse, Paine, Robert 3
    J Biol Chem, 2014;289(7):4095-105.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Oncolytic viral therapy with a combination of HF10, a herpes simplex virus type 1 variant and granulocyte-macrophage colony-stimulating factor for murine ovarian cancer.
    Authors: Goshima F, Esaki S, Luo C, Kamakura M, Kimura H, Nishiyama Y
    Int J Cancer, 2014;134(12):2865-77.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. Protein malnutrition induces bone marrow mesenchymal stem cells commitment to adipogenic differentiation leading to hematopoietic failure.
    Authors: Cunha M, Lima F, Vinolo M, Hastreiter A, Curi R, Borelli P, Fock R
    PLoS ONE, 2013;8(3):e58872.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Deletion of tristetraprolin caused spontaneous reactive granulopoiesis by a non-cell-autonomous mechanism without disturbing long-term hematopoietic stem cell quiescence.
    Authors: Kaplan IM, Morisot S, Heiser D, Cheng WC, Kim MJ, Civin CI
    J. Immunol., 2011;186(5):2826-34.
    Species: Mouse
    Sample Types: Plasma
  12. Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10.
    Authors: Wang H, Cheng F
    J. Immunol., 2011;186(0):3986.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Keratinocyte Growth Factor Augments Pulmonary Innate Immunity through Epithelium-driven, GM-CSF-dependent Paracrine Activation of Alveolar Macrophages.
    Authors: Wu H, Suzuki T, Carey B, Trapnell BC, McCormack FX
    J. Biol. Chem., 2011;286(17):14932-40.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. Bcl3 prevents acute inflammatory lung injury in mice by restraining emergency granulopoiesis.
    Authors: Kreisel D, Sugimoto S, Tietjens J, Zhu J, Yamamoto S, Krupnick AS, Carmody RJ, Gelman AE
    J. Clin. Invest., 2011;121(1):265-76.
    Species: Mouse
    Sample Types: Serum
  15. Macrophage tumor necrosis factor-alpha induces epithelial expression of granulocyte-macrophage colony-stimulating factor: impact on alveolar epithelial repair.
    Authors: Cakarova L, Marsh LM, Wilhelm J, Mayer K, Grimminger F, Seeger W, Lohmeyer J, Herold S
    Am. J. Respir. Crit. Care Med., 2009;180(6):521-32.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Novel immunocompetent murine tumor model for evaluation of conditionally replication-competent (oncolytic) murine adenoviral vectors.
    Authors: Robinson M, Li B, Ge Y, Ko D, Yendluri S, Harding T, VanRoey M, Spindler KR, Jooss K
    J. Virol., 2009;83(8):3450-62.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. Simultaneous absence of surfactant proteins A and D increases lung inflammation and injury after allogeneic HSCT in mice.
    Authors: Gram K, Yang S, Steiner M, Somani A, Hawgood S, Blazar BR, Panoskaltsis-Mortari A, Haddad IY
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(2):L167-75.
    Species: Mouse
    Sample Types: BALF
  18. IL-7 induces myelopoiesis and erythropoiesis.
    Authors: Aiello FB, Keller JR, Klarmann KD, Dranoff G, Mazzucchelli R, Durum SK
    J. Immunol., 2007;178(3):1553-63.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. Lentiviral vector-mediated autonomous differentiation of mouse bone marrow cells into immunologically potent dendritic cell vaccines.
    Authors: Koya RC, Kimura T, Ribas A, Rozengurt N, Lawson GW, Faure-Kumar E, Wang HJ, Herschman H, Kasahara N, Stripecke R
    Mol. Ther., 2007;15(5):971-80.
    Species: Mouse
    Sample Types: Serum
  20. Administration of cyclooxygenase-2 inhibitor reduces joint inflammation but exacerbates osteopenia in IL-1 alpha transgenic mice due to GM-CSF overproduction.
    Authors: Niki Y, Takaishi H, Takito J, Miyamoto T, Kosaki N, Matsumoto H, Toyama Y, Tada N
    J. Immunol., 2007;179(1):639-46.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Organ-specific role of MyD88 for gene regulation during polymicrobial peritonitis.
    Authors: Weighardt H, Mages J, Jusek G, Kaiser-Moore S, Lang R, Holzmann B
    Infect. Immun., 2006;74(6):3618-32.
    Species: Mouse
    Sample Types: Tissue Homogenates
  22. GM-CSF and the impaired pulmonary innate immune response following hyperoxic stress.
    Authors: Baleeiro CE, Christensen PJ, Morris SB, Mendez MP, Wilcoxen SE, Paine R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(6):L1246-55.
    Species: Mouse
    Sample Types: Tissue Homogenates
  23. Expression of CCL20 and granulocyte-macrophage colony-stimulating factor, but not Flt3-L, from modified vaccinia virus ankara enhances antiviral cellular and humoral immune responses.
    Authors: Chavan R, Marfatia KA, An IC, Garber DA, Feinberg MB
    J. Virol., 2006;80(15):7676-87.
  24. A novel polysaccharide of black soybean promotes myelopoiesis and reconstitutes bone marrow after 5-flurouracil- and irradiation-induced myelosuppression.
    Authors: Liao HF, Chen YJ, Yang YC
    Life Sci., 2005;77(4):400-13.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Endogenous granulocyte-macrophage colony-stimulating factor overexpression in vivo results in the long-term recruitment of a distinct dendritic cell population with enhanced immunostimulatory function.
    Authors: Miller G, Pillarisetty VG, Shah AB, Lahrs S, Xing Z, DeMatteo RP
    J. Immunol., 2002;169(6):2875-85.
    Species: Mouse
    Sample Types: Serum
  26. Interleukin 3 and granulocyte-macrophage colony-stimulating factor are not required for induction of chronic myeloid leukemia-like myeloproliferative disease in mice by BCR/ABL.
    Authors: Li S, Gillessen S, Tomasson MH, Dranoff G, Gilliland DG, Van Etten RA
    Blood, 2001;97(5):1442-50.
    Species: Mouse
    Sample Types: Serum
  27. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
    Authors: Shih CC, Hu MC, Hu J
    Blood, 2000;95(6):1957-66.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Reviews for Mouse GM-CSF Quantikine ELISA Kit

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Mouse GM-CSF Quantikine ELISA Kit
By Anonymous on 02/20/2019
Application: Sample Tested: Serum

Mouse GM-CSF Quantikine ELISA Kit
By Anonymous on 05/11/2018
Application: Sample Tested: Pancreatic cancer cells