Mouse Hemopexin Antibody

Catalog # Availability Size / Price Qty
MAB7007
MAB7007-SP

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Detection of Mouse Hemopexin by Western Blot.
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Product Details
Citations (2)
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Mouse Hemopexin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Hemopexin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Hemopexin is observed.
Source
Monoclonal Rat IgG2A Clone # 781941
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Hemopexin
Met1-Gln460
Accession # Q91X72
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Hemopexin antibody by Western Blot. View Larger

Detection of Mouse Hemopexin by Western Blot. Western blot shows mouse plasma. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Hemopexin Monoclonal Antibody (Catalog # MAB7007) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Hemopexin at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hemopexin

HPX (Hemopexin) is a 60-68 kDa secreted member of the hemopexin family of molecules. Although it is principally expressed by hepatocytes, it is also reportedly secreted by macrophages, neurons, astrocytes, renal mesangial cells and fibroblasts. It is considered an acute phase protein, and demonstrates a number of effects. Principally, it binds free and albumin-bound heme, neutralizing its potential for oxidative damage to cell membranes. Heme is removed from HPX internally following binding of the HPX:heme complex to CD91 on hepatocytes and macrophages. HPX also dampens the proinflammatory response of macrophages to TLR2 and TLR4 activation. And HPX also shows serine protease activity which may impact cell surface proteins and ECM. Mature mouse HPX is a 437 amino acid (aa) glycoprotein (aa 24-460). It contains five consecutive HPX-like domains (aa 56-349) that likely impart binding-partner specificity to HPX. There are multiple potential isoform variants. One shows a two aa substitution for aa 134-460, a second contains a deletion of aa 60-100, a third possesses a 14 aa substitution for aa 235-460, while a fourth shows a two aa substitution for aa 153-460. Mature mouse HPX shares 92% and 77% aa identity with rat and human HPX, respectively.

Entrez Gene IDs
3263 (Human); 15458 (Mouse); 58917 (Rat)
Alternate Names
Beta-1B-glycoprotein; FLJ56652; Hemopexin; HPX; HX

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Citations for Mouse Hemopexin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Sex-dependent acrolein sensitivity in mice is associated with differential lung cell, protein, and transcript changes
    Authors: K Bein, RL Birru, H Wells, TP Larkin, T Ge, GD Leikauf
    Physiological Reports, 2021-10-01;9(19):e14997.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Control of Oxidative Stress and Inflammation in Sickle Cell Disease with the Nrf2 Activator Dimethyl Fumarate
    Authors: John D. Belcher, Chunsheng Chen, Julia Nguyen, Ping Zhang, Fuad Abdulla, Phong Nguyen et al.
    Antioxidants & Redox Signaling

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