Mouse HSP10/EPF Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2584
Detection of Mouse HSP10/EPF by Western Blot.
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Mouse HSP10/EPF Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects endogenous levels of mouse HSP10/EPF in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse HSP10/EPF
Ala2-Asp102
Accession # Q64433
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse HSP10/EPF antibody by Western Blot. View Larger

Detection of Mouse HSP10/EPF by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, C2C12 mouse myoblast cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse HSP10/EPF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2584) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSP10/EPF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP10/EPF

The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Protein 10 (HSP10), also known as chaperonin 10 (Cpn10), HSPE1, and Early Pregnancy Factor (EPF), is a 10 kDa heat shock protein that functions within the cell as a molecular chaperone and is associated with HSP60 in the mitochondria. Early pregnancy factor (EPF) has been identified as an extracellular homologue of HSP10.

Long Name
Heat Shock 10 kDa Protein 1
Entrez Gene IDs
3336 (Human); 69253 (Mouse)
Alternate Names
10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; Chaperonin 10 Homolog; Chaperonin 10; CPN10HSP10; Early-pregnancy factor; EPF; GroES; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1 (chaperonin 10); HSP10; HSPE1

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