Mouse IFN-alpha All Subtype Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
MFNAS0
Mouse IFN-alpha All Subtype Quantikine Natural Linearity Competitor Comparison
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Mouse IFN-alpha All Subtype Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
3.79 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine™ Mouse IFN-alpha All Subtype immunoassay is a 3.5 hour solid-phase ELISA designed to measure IFN-alpha in mouse cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant mouse IFN-alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IFN-alpha showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-alpha.

Recovery

The recovery of mouse IFN-alpha spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 91-106
EDTA Plasma (n=4) 105 91-121
Heparin Plasma (n=4) 106 96-120
Serum (n=4) 102 86-118

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IFN-alpha were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Mouse IFN-alpha  All Subtype ELISA Linearity

Scientific Data

Mouse IFN-alpha All Subtype Quantikine Natural Linearity Competitor Comparison IFN-alpha samples containing a high concentration in various matrices are diluted with appropriate Calibrator Diluent to produce sample values within the dynamic range of the assay. R&D Systems natural linearity is between 98%-103% compared to 125%-180% for the top competitor.

Mouse IFN-alpha All Subtype Quantikine ELISA Spiked Linearity Competitor Comparison IFN-alpha is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. R&D Systems linearity is between 88%-92% compared to 132%-201% for the top competitor.

Mouse IFN-alpha All Subtype Quantikine ELISA Spiked Recovery IFN-alpha is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. R&D Systems recovery is 99%.

Mouse IFN-alpha All Subtype ELISA Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name:
Interferon alpha
Entrez Gene IDs:
3440 (Human)
Alternate Names:
IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D

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