Mouse IFN-alpha / beta  R1 Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta  R1 Antibody.
1 Image
Product Details
Citations (2)
Supplemental Products

Mouse IFN-alpha / beta  R1 Antibody Summary

Species Reactivity
Detects mouse IFN-alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) IFN‑ alpha / beta  R2, rmIFN-gamma R1, and rmIFN-gamma R2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse IFN-alpha / beta R1
Accession # P33896
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse IFN-alpha / beta  R1 (Catalog # 3039-AB)
Measured by its ability to neutralize IFN‑ alpha / beta  R1-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta  R1 Antibody. View Larger

Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ alpha / beta R1 Antibody. Recombinant Mouse Limitin (Catalog # 1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IFN-a/ beta R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3039). The ND50 is typically 1-4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R1

IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1-3). IFN-alpha / beta R1, in association with IFN-alpha / beta R2, is required for propagating antiviral signal transduction triggered by IFN-alpha and IFN-beta (4, 5). The mouse IFN-alpha / beta R1 cDNA encodes a 590 amino acid (aa) precursor including a 26 aa signal sequence, a 403 aa extracellular domain (ECD), a 20 aa transmembrane segment, and a 141 aa cytoplasmic domain (6). The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. The ECD of mouse IFN-alpha / beta R1 shares 47-48% aa identity with that of human, bovine, porcine, and ovine IFN-alpha / beta R1. IFN-alpha / beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN-alpha / beta R2. Ligands associate with IFN-alpha / beta R2, and this complex subsequently forms a stable ternary assembly with IFN-alpha / beta R1 (7, 8). IFN-alpha / beta R1 also associates with IFN-gamma R2 even in the absence of IFN-gamma stimulation (5). Tyrosine phosphorylation within the juxtamembrane cytoplasmic domain of IFN-alpha / beta R1 provides a docking site for the SH2 domains of Tyk2 and STAT2 (9-11). Tyk2 can directly phosphorylate IFN-alpha / beta R1 (10). Tyk2 also increases the level of cell surface expression of IFN-alpha / beta R1 by preventing constitutive internalization (12). Human IFN-alpha / beta R1 contains a nuclear localization signal in its ECD which is required for receptor translocation to the nucleus following interaction with ligand (13).

  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Kotenko, S.V. and J.A. Langer (2004) Int. Immunopharmacol. 4:593.
  3. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
  4. Hwang, S.Y. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11284.
  5. Takaoka, A. et al. (2000) Science 288:2357.
  6. Uze, G. et al. (1992) Proc. Natl. Acad. Sci. USA 89:4774.
  7. Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
  8. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
  9. Krishnan, K. et al. (1998) J. Biol. Chem. 273:19495.
  10. Yan, H. et al. (1996) Mol. Cell. Biol. 16:2074.
  11. Yan, H. et al. (1996) EMBO J. 15:1064.
  12. Ragimbeau, J. et al. (2003) EMBO J. 22:537.
  13. Subramaniam, P.S. and H.M. Johnson (2004) FEBS Lett. 578:207.
Long Name
Interferon alpha/beta Receptor 1
Entrez Gene IDs
3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)
Alternate Names
alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1

Product Datasheets

You must select a language.


Citations for Mouse IFN-alpha / beta  R1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. UBXN3B Controls Immunopathogenesis of Arthritogenic Alphaviruses by Maintaining Hematopoietic Homeostasis
    Authors: T Geng, D Yang, T Lin, JG Cahoon, P Wang
    MBio, 2022-11-15;0(0):e0268722.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Toll-like receptor (TLR)2 and TLR3 synergy and cross-inhibition in murine myeloid dendritic cells.
    Authors: Vanhoutte F, Paget C, Breuilh L, Fontaine J, Vendeville C, Goriely S, Ryffel B, Faveeuw C, Trottein F
    Immunol. Lett., 2007-12-17;116(1):86-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


Reviews for Mouse IFN-alpha / beta  R1 Antibody

There are currently no reviews for this product. Be the first to review Mouse IFN-alpha / beta  R1 Antibody and earn rewards!

Have you used Mouse IFN-alpha / beta  R1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review