IFN-beta (Interferon-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.
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Mouse IFN-beta Quantikine ELISA Kit
R&D Systems | Catalog # MIFNB0
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
1.89 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Mouse IFN-beta Quantikine ELISA Kit
The Quantikine® Mouse IFN-beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IFN-beta in cell culture supernates, serum, and plasma. It contains
HEK293-expressed recombinant mouse IFN-beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse IFN-beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-beta.
HEK293-expressed recombinant mouse IFN-beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse IFN-beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-beta.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse IFN-beta.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Ten serum samples, 5 EDTA plasma, and 5 heparin plasma samples were
evaluated for the presence of mouse IFN-beta in this assay. No detectable levels were observed.
ND=Non-detectable
Cell Culture Supernates - - NIH-3T3 mouse embyronic fibroblast cells were cultured in DMEM
supplemented with 10% fetal bovine serum and grown to approximately 90% confluency.
Cells were unstimulated or stimulated with 10 μg/mL Poly I:C delivered via Lipofectamine for
24 hours. Aliquots of the cell culture supernates were removed and assayed for levels of mouse
IFN-beta.
| Condition | (pg/mL) |
| Unstimulated | ND |
| Stimulated | 1768 |
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 51.5 | 180 | 437 | 52.6 | 173 | 441 |
| Standard Deviation | 180 | 5.84 | 13.7 | 5.28 | 10.0 | 27.2 |
| CV% | 437 | 3.2 | 3.1 | 10.0 | 5.8 | 6.2 |
Recovery for Mouse IFN-beta Quantikine ELISA Kit
The recovery of mouse IFN-beta spiked into various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 88-106 |
| EDTA Plasma (n=4) | 99 | 89-106 |
| Heparin Plasma (n=4) | 94 | 83-100 |
| Serum (n=4) | 94 | 89-97 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of mouse IFN-beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse IFN-beta Quantikine ELISA Kit
Mouse IFN-beta ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-beta
Long Name
Interferon beta
Alternate Names
IFB, IFF, IFNB, IFNB1, IFNbeta
Gene Symbol
IFNB1
Additional IFN-beta Products
Product Documents for Mouse IFN-beta Quantikine ELISA Kit
Product Specific Notices for Mouse IFN-beta Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Mouse IFN-beta Quantikine ELISA Kit
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Sample Tested: Mouse spleenocytesVerified Customer | Posted 02/07/2023
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Sample Tested: EDTA PlasmaVerified Customer | Posted 11/15/2022
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Sample Tested: Cell culture supernatantVerified Customer | Posted 10/26/2022Cell culture supernatants transfected with human and mouse IFNb on BHK-21 cells
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Sample Tested: IC-21 mouse macrophage cell lineVerified Customer | Posted 11/15/2019
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Sample Tested: Cell Culture SupernatesVerified Customer | Posted 10/19/2017
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Sample Tested: Serum and Cell culture supernatantVerified Customer | Posted 09/07/2017
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