Mouse IFN-beta Quantikine ELISA Kit

  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    1.89 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse IFN-beta.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine® Mouse IFN-beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IFN-beta in cell culture supernates, serum, and plasma. It contains
HEK293-expressed recombinant mouse IFN-beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse IFN-beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-beta.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean51.518043752.6173441
Standard Deviation1805.8413.75.281027.2
CV%4373.23.1105.86.2

Recovery

The recovery of mouse IFN-beta spiked into various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 88-106
EDTA Plasma (n=4) 99 89-106
Heparin Plasma (n=4) 94 83-100
Serum (n=4) 94 89-97
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of mouse IFN-beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Mouse IFN-beta Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-beta
Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.
    • Long Name
      Interferon beta
    • Entrez Gene IDs
      3456 (Human); 15977 (Mouse); 24481 (Rat);
    • Alternate Names
      Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956;
    Related Research Areas
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    Quantikine Wash Buffer 1

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