Mouse IFN‑ gamma Antibody

Name: Mouse IFN‑ gamma Antibody
Brand: R&D Systems
SKU: MAB785
Price: 499 USD
Availability: InStock

R&D Systems | Catalog # MAB785

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Citations (30)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

ELISA Capture, ELISA Development, ELISA Development (Capture), ELISpot Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A and IgG_2B Cocktail Clone # 37801/37875

View all IFN-gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IFN-gamma

Specificity

Detects mouse IFN-gamma in ELISAs. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) C10, rmG-CSF, rmGM‑CSF, recombinant human IFN‑ gamma, rmIL-1 alpha, rmIL-1 beta, rmIL-2, rmIL-3, rmIL-4, rmIL-5, rmIL-6, rmIL-7, rmIL-9, rmIL-10, rmIL-10 R, rmIL-12, rmIL-13, rmJE, rmKC, rmLIF, rmM-CSF, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-2, rmSCF, rmTNF-alpha, rmTpo, and rmVEGF.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A and IgG_2B Cocktail

Scientific Data Images for Mouse IFN‑ gamma Antibody

Detection of Mouse IFN-gamma by ELISA

A27L-specific cell-mediated immune response against recombinant VVWR determined by ELISPOT analysis.(A) Each group consisted of four female Balb/C mice immunized three times, two weeks apart via intramuscular injection as follows: Naïve (negative control), pVax1 (empty vector backbone control), pA27L (VVWR envelope protein), pA27L+I (VVWR envelope protein + imiquimod), pA27L + R (VVWR envelope protein + resiquimod), pA27LOPT (optimized sequence of the A27L gene) and pA27LOPT+I (the optimized sequence of A27L + imiquimod). (B) Identification of A27L dominant epitopes. Antigen-specific IFN-gamma ELISPOT in splenocytes from mice (immunized three times, two weeks apart via intramuscular injection) in response to VVWR A27 individual 3-mer overlapped 15-mer overlapping peptides. Each experiment was performed three times and the immune responses among groups of mice are presented as the mean ± standard error of the mean (SEM). A p value of less than 0.05 was considered significant. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25875833), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IFN‑ gamma Antibody

Mouse IFN-gamma Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse IFN‑ gamma Biotinylated Antibody (Catalog # BAF485)
Standard: Recombinant Mouse IFN-gamma Protein (Catalog # 485-MI)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IFN-gamma

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. It shares 86% amino acid (aa) sequence identity with rat IFN-gamma, 38-44% with bovine, canine, cotton rat, equine, feline, human, porcine and rhesus IFN‑ gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.