Mouse IgD Antibody

Catalog # Availability Size / Price Qty
MAB9858-100
MAB9858-SP
Detection of IgD in Mouse Splenocytes by Flow Cytometry
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Mouse IgD Antibody Summary

Species Reactivity
Mouse
Specificity
Reacts with the delta chain of Mouse IgD of all haplotypes and does not cross-react with other immunoglobulin isotypes.
Source
Monoclonal Rat IgG2A Clone # 11-26C
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse IgD
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IgD antibody in Mouse Splenocytes antibody by Flow Cytometry View Larger

Detection of IgD in Mouse Splenocytes by Flow Cytometry Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse IgD Monoclonal Antibody (Catalog # MAB9858) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgD

 Immunoglobulin D (IgD) is an antibody isotype typically expressed in the plasma membranes of naïve B cells, usually co-expressed with IgM. IgD is also found secreted in small amounts in serum. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta class, and two Ig light chains.

Long Name
Immunoglobulin D
Entrez Gene IDs
3495 (Human); 380797 (Mouse)
Alternate Names
IgD; Igh-5; Ighd; Immunoglobulin D; Immunoglobulin Delta

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