Mouse IgE Antibody

Catalog # Availability Size / Price Qty
MAB9935-100
Detection of Mouse IgE by Western Blot.
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Mouse IgE Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgE in Direct Elisa and Western blots.
Source
Monoclonal Rat IgG2A Clone # 343527
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse IgE
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse IgE by Western Blot. View Larger

Detection of Mouse IgE by Western Blot. Western blot shows lysates of purified Mouse IgM (negative control), purified Mouse IgA (negative control), purified Mouse IgG(negative control), and human purified Mouse IgE. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IgE Monoclonal Antibody (Catalog # MAB9935) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IgE at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgE

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin E
Alternate Names
IgE; immunoglobulin heavy constant epsilon

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