Mouse IGFBP-L1 Antibody

Catalog #: AF4130
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4130
AF4130-SP

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Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IGFBP-L1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IGFBP-L1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human IGFBP-L1 is observed and less than 1% cross-reactivity with recombinant mouse IGFBP-7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-L1
Leu18-Leu270
Accession # Q80W15

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IGFBP-L1 (Catalog # 4130-BL)

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of embyronic mouse brain (E13.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-L1

Insulin-like Growth Factor Binding Protein-Like 1 (IGFBP‑L1), also known as IGFBP-rP4, is a 38 kDa secreted member of the IGFBP superfamily of proteins. IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins (1). IGFBP-L1 contains an N-terminal IGFBP motif, a Kazal-type serine protease inhibitor region, and a C‑terminal Ig-like domain (2, 3). Mature mouse IGFBP-L1 shares 76% and 96% amino acid (aa) sequence identity with human and rat IGFBP-L1, respectively. It shares 38%-41% aa sequence identity with mouse IGFBP-7 and IGFBP-rp10 and 20%‑24% aa sequence identity with mouse IGFBP-1, -2, -3, -4, -5, and -6. IGFBP-L1 is most highly expressed in testis and brain, while lower levels are more broadly expressed (3). IGFBP-L1 is expressed in regionally and temporally distinct patterns during forebrain development (4). IGFBP-L1 inhibits cell proliferation in vitro and is downregulated in many colon and lung tumors (3). Transcriptional downregulation by promoter methylation is predictive of breast cancer aggressiveness (5).

References

Firth, S.M. and R.C. Baxter (2002) Endocr. Rev. 23:824.
Accession # Q80W15.
Cai, Z. et al. (2005) Biochem. Biophys. Res. Commun. 331:261.
Gonda, Y. et al. (2007) Gene Expr. Patterns 7:431.
Smith, P. et al. (2007) Clin. Cancer Res. 13:4061.

Long Name

Insulin-like Growth Factor Binding Protein-like 1

Entrez Gene IDs

347252 (Human); 75426 (Mouse)

Alternate Names

bA113O24.1; IGFBPL1 insulin-like growth factor binding protein-like 1; IGFBPL1; IGFBP-L1; IGFBP-RP4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IGFBP-L1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Neural precursor cells tune striatal connectivity through the release of IGFBPL1
Authors: E Butti, S Cattaneo, M Bacigalupp, M Cambiaghi, GM Scotti, E Brambilla, F Ruffini, G Sferruzza, M Ripamonti, F Simeoni, L Cacciaguer, A Zanghì, A Quattrini, R Fesce, P Panina-Bor, F Giannese, D Cittaro, T Kuhlmann, P D'Adamo, MA Rocca, S Taverna, G Martino
Nature Communications, 2022-12-08;13(1):7579.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC/IF
IGFBPL1 Regulates Axon Growth through IGF-1-mediated Signaling Cascades
Authors: C Guo, KS Cho, Y Li, K Tchedre, C Antolik, J Ma, J Chew, TP Utheim, XA Huang, H Yu, MTA Malik, N Anzak, DF Chen
Sci Rep, 2018-02-01;8(1):2054.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC