Mouse IgG2A Isotype Control

Catalog # Availability Size / Price Qty
MAB0031
Detection of MouseIgG Control by Flow Cytometry
1 Image
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (2)

Mouse IgG2A Isotype Control Summary

Specificity
Recognizes KLH (Keyhole Limpet Hemocyanin).  In immunohistochemistry or flow cytometry of a variety of cell types or tissues, no background staining is observed.
Source
Monoclonal Mouse IgG2A Clone # 133304
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for flow cytometry, immunohistochemistry, or immunocytochemistry experiments. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of MouseIgG Control by Flow Cytometry HT1080 human fibrosarcoma cell line was stained with MouseAnti-Human ULBP-2/5/6 Monoclonal Antibody
(Catalog # MAB1298,filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB0031,open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgGSecondary Antibody
(Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2A

IgG is the most abundant of the five classes of mammalian immunoglobulins in serum. IgG molecules (~150 kDa) consist of two ~50 kDa heavy chains and two 25 kDa light chains connected by disulfide bonds. Antibody specificity is conferred by a combination of somatic rearrangement and hypermutation within variable regions. Mouse IgG isotypes IgG1, IgG2a and IgG2b share approximately 64-78% amino acid sequence identity within the heavy chain constant region domains (CH1, CH2 and CH3) and may vary in their interaction with complement, NK and mast cells, induction or inhibition by specific cytokines, and ability to cross the placenta.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

Product Datasheets

Citations for Mouse IgG2A Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Recognition of bacterial signal peptides by mammalian formyl peptide receptors: a new mechanism for sensing pathogens.
    Authors: Bufe B, Schumann T, Kappl R, Bogeski I, Kummerow C, Podgorska M, Smola S, Hoth M, Zufall F
    J Biol Chem, 2015;290(12):7369-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Structure of acidic pH dengue virus showing the fusogenic glycoprotein trimers.
    Authors: Zhang X, Sheng J, Austin S, Hoornweg T, Smit J, Kuhn R, Diamond M, Rossmann M
    J Virol, 2015;89(1):743-50.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Isotype control
  3. In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
    Authors: Berglin L, Bergquist A, Johansson H, Glaumann H, Jorns C, Lunemann S, Wedemeyer H, Ellis E, Bjorkstrom N
    PLoS ONE, 2014;9(8):e105375.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Isotype control
  4. Development of a highly protective combination monoclonal antibody therapy against Chikungunya virus.
    Authors: Pal, Pankaj, Dowd, Kimberly, Brien, James D, Edeling, Melissa, Gorlatov, Sergey, Johnson, Syd, Lee, Iris, Akahata, Wataru, Nabel, Gary J, Richter, Mareike, Smit, Jolanda, Fremont, Daved H, Pierson, Theodore, Heise, Mark T, Diamond, Michael
    PLoS Pathog, 2013;9(4):e1003312.
    Species: Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Types: Whole Cells
    Applications: Func
  5. CXCR7 protein is not expressed on human or mouse leukocytes.
    Authors: Berahovich RD, Zabel BA, Penfold ME, Lewen S, Wang Y, Miao Z, Gan L, Pereda J, Dias J, Slukvin II, McGrath KE, Jaen JC, Schall TJ
    J. Immunol., 2010;185(9):5130-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  6. Susceptibility of human testis to human immunodeficiency virus-1 infection in situ and in vitro.
    Authors: Roulet V, Satie AP, Ruffault A, Le Tortorec A, Denis H, Guist'hau O, Patard JJ, Rioux-Leclerq N, Gicquel J, Jegou B, Dejucq-Rainsford N
    Am. J. Pathol., 2006;169(6):2094-103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ctrl
  7. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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Reviews for Mouse IgG2A Isotype Control

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Mouse IgG2A Isotype Control
By Anonymous on 08/17/2016
Application: ICC/IF Sample Tested: ?TC-6 mouse beta cell insulinoma cell line Species: Human,Mouse

PFA fixation. Used between 5ug/mL and 0.5ug/mL. No signals until respectively 500 and 750msec of acquisition.


Mouse IgG2A Isotype Control
By Anonymous on 02/22/2016
Application: Flow Sample Tested: Human Placenta Species: Mouse,Human