Detection of MouseIgG Control by Flow Cytometry HT1080 human fibrosarcoma cell line was stained with MouseAnti-Human ULBP-2/5/6 Monoclonal Antibody (Catalog # MAB1298,filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB0031,open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgGSecondary Antibody (Catalog # F0101B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IgG is the most abundant of the five classes of mammalian immunoglobulins in serum. IgG molecules (~150 kDa) consist of two ~50 kDa heavy chains and two 25 kDa light chains connected by disulfide bonds. Antibody specificity is conferred by a combination of somatic rearrangement and hypermutation within variable regions. Mouse IgG isotypes IgG1, IgG2a and IgG2b share approximately 64-78% amino acid sequence identity within the heavy chain constant region domains (CH1, CH2 and CH3) and may vary in their interaction with complement, NK and mast cells, induction or inhibition by specific cytokines, and ability to cross the placenta.
Immunoglobulin G2A Fc
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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