Mouse IgG2A Isotype Control

(7 citations)
(2 Reviews)
  
  • Specificity
    Recognizes KLH (Keyhole Limpet Hemocyanin).  In immunohistochemistry or flow cytometry of a variety of cell types or tissues, no background staining is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 133304
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for flow cytometry, immunohistochemistry, or immunocytochemistry experiments. Use at the same concentration as the detection antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of MouseIgG Control by Flow Cytometry HT1080 human fibrosarcoma cell line was stained with MouseAnti-Human ULBP-2/5/6 Monoclonal Antibody
(Catalog # MAB1298,filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB0031,open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgGSecondary Antibody
(Catalog # F0101B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2A
IgG is the most abundant of the five classes of mammalian immunoglobulins in serum. IgG molecules (~150 kDa) consist of two ~50 kDa heavy chains and two 25 kDa light chains connected by disulfide bonds. Antibody specificity is conferred by a combination of somatic rearrangement and hypermutation within variable regions. Mouse IgG isotypes IgG1, IgG2a and IgG2b share approximately 64-78% amino acid sequence identity within the heavy chain constant region domains (CH1, CH2 and CH3) and may vary in their interaction with complement, NK and mast cells, induction or inhibition by specific cytokines, and ability to cross the placenta.
  • Long Name:
    Immunoglobulin G2A Fc
  • Alternate Names:
    IgG2A
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Species
Applications
Sample Type
  1. Structure of acidic pH dengue virus showing the fusogenic glycoprotein trimers.
    Authors: Zhang X, Sheng J, Austin S, Hoornweg T, Smit J, Kuhn R, Diamond M, Rossmann M
    J Virol, 2015;89(1):743-50.
    Species: Hamster
    Sample Type: Whole Cells
    Application: Isotype control
  2. Recognition of bacterial signal peptides by mammalian formyl peptide receptors: a new mechanism for sensing pathogens.
    Authors: Bufe B, Schumann T, Kappl R, Bogeski I, Kummerow C, Podgorska M, Smola S, Hoth M, Zufall F
    J Biol Chem, 2015;290(12):7369-87.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
    Authors: Berglin L, Bergquist A, Johansson H, Glaumann H, Jorns C, Lunemann S, Wedemeyer H, Ellis E, Bjorkstrom N
    PLoS ONE, 2014;9(8):e105375.
    Species: Human
    Sample Type: Whole Tissue
    Application: Isotype control
  4. Development of a highly protective combination monoclonal antibody therapy against Chikungunya virus.
    Authors: Pal, Pankaj, Dowd, Kimberly, Brien, James D, Edeling, Melissa, Gorlatov, Sergey, Johnson, Syd, Lee, Iris, Akahata, Wataru, Nabel, Gary J, Richter, Mareike, Smit, Jolanda, Fremont, Daved H, Pierson, Theodore, Heise, Mark T, Diamond, Michael
    PLoS Pathog, 2013;9(4):e1003312.
    Species: Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Type: Whole Cells
    Application: Functional Assay
  5. CXCR7 protein is not expressed on human or mouse leukocytes.
    Authors: Berahovich RD, Zabel BA, Penfold ME, Lewen S, Wang Y, Miao Z, Gan L, Pereda J, Dias J, Slukvin II, McGrath KE, Jaen JC, Schall TJ
    J. Immunol., 2010;185(9):5130-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Susceptibility of human testis to human immunodeficiency virus-1 infection in situ and in vitro.
    Authors: Roulet V, Satie AP, Ruffault A, Le Tortorec A, Denis H, Guist'hau O, Patard JJ, Rioux-Leclerq N, Gicquel J, Jegou B, Dejucq-Rainsford N
    Am. J. Pathol., 2006;169(6):2094-103.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 13  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Fluorescein-conjugated Antibody

Ctrl IC003F 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

Ctrl IC003G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A PerCP-conjugated Isotype Control

Ctrl IC003C 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC003N  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Alexa Fluor® 405-conjugated Isotype Control

Ctrl IC003V
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Isotype Control

Ctrl MAB003R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Alexa Fluor® 594-conjugated Isotype Control

Ctrl IC003T
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Alexa Fluor® 750-conjugated Isotype Control

Ctrl IC003S
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Biotinylated Isotype Control

Ctrl IC003B
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC003R
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 2 reviews)

Have you used Mouse IgG2A Isotype Control?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Mouse IgG2A Isotype Control.

We have 2 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Flow Cytometry.
Filter by Ratings
Excellent
(2)
All Ratings
Filter by Applications
ICC/IF
(1)
Flow
(1)
All Applications
Filter by Species
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
 ICC/IF Anonymous 08/17/2016
Show

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample Tested?TC-6 mouse beta cell insulinoma cell line

Other Experimental Details

Other Experimental DetailsPFA fixation. Used between 5ug/mL and 0.5ug/mL. No signals until respectively 500 and 750msec of acquisition.
  Excellent
 Flow Anonymous 02/22/2016
Show

Summary

ApplicationFlow Cytometry
Sample TestedHuman Placenta

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information