Mouse IgG2B Isotype Control

(32 citations)
(1 Review)
  
  • Specificity
    Selected for use as mouse IgG2B isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT II (rhMIP-1 beta ), rhBLC/BCA-1, rmBLC, rhBRAK, rmBRAK, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC‑2 beta, rrCINC-3, rhCK beta 8-1, rmCRG-2, rmCTACK, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin 2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmI-TAC, rmJE, rmKC, rhLeukotactin-1, rrLIX, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma, rhMIP-1δ, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, recombinant viral (rv) MIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF‑1 alpha, rmSDF‑1 alpha, rhSDF‑1 beta, rhTarc, rmTarc, rmTCA-3, rhTeck, rmTeck, rhVIC, or rmVIC.
  • Source
    Monoclonal Mouse IgG2B Clone # 20116
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of MouseIgG Control by Flow Cytometry K562 human chronic myelogenous leukemia cell line wasstained with Mouse Anti-Human ITFG1 Monoclonal Antibody (Catalog #MAB89001,filled histogram) or Mouse IgG Isotype Control Antibody (Catalog #MAB004,open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgGSecondary Antibody (Catalog # F0101B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2B Fc
  • Alternate Names:
    IgG2B
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions
    Authors: S Ikeda, R Kawahara-M, H Iwata, M Sugimoto, S Kume
    Sci Rep, 2017;7(1):3800.
    Species: Bovine
    Sample Type: Cell Lysates
    Application: Ctrl
  2. Specific pathways mediating inflammasome activation by Candida parapsilosis
    Authors: A Tóth, E Zajta, K Csonka, C Vágvölgyi, MG Netea, A Gácser
    Sci Rep, 2017;7(0):43129.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  3. Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis
    Authors: P Dmitriev, E Kiseleva, O Kharchenko, E Ivashkin, A Pichugin, P Dessen, T Robert, F Coppée, A Belayew, G Carnac, D Laoudj-Che, M Lipinski, A Vasiliev, YS Vassetzky
    Oncotarget, 2016;7(40):65090-65108.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  4. Novel chemoimmunotherapeutic strategy for hepatocellular carcinoma based on a genome-wide association study
    Sci Rep, 2016;6(0):38407.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10
    Sci Rep, 2016;5(0):37366.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. A Model to Explain How the Bacille Calmette Gu�rin (BCG) Vaccine Drives Interleukin-12 Production in Neonates
    PLoS ONE, 2016;11(8):e0162148.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Ctrl
  7. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses
    Authors: Wan-Chien Cheng
    Eur J Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  8. Role and species-specific expression of colon T cell homing receptor GPR15 in colitis.
    Authors: Nguyen L, Pan J, Dinh T, Hadeiba H, O'Hara E, Ebtikar A, Hertweck A, Gokmen M, Lord G, Jenner R, Butcher E, Habtezion A
    Nat Immunol, 2015;16(2):207-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Ctrl
  9. Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
    Authors: Cox S, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal J, Saudemont A
    Eur J Immunol, 2015;45(8):2324-34.
    Species: Human
    Sample Type: Plasma
    Application: IP
  10. TSLP production by dendritic cells is modulated by IL-1beta and components of the endoplasmic reticulum stress response.
    Authors: Elder M, Webster S, Williams D, Gaston J, Goodall J
    Eur J Immunol, 2015;46(2):455-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut Ctrl
  11. Human dendritic cell DC-SIGN and TLR-2 mediate complementary immune regulatory activities in response to Lactobacillus rhamnosus JB-1.
    Authors: Konieczna P, Schiavi E, Ziegler M, Groeger D, Healy S, Grant R, O'Mahony L
    PLoS ONE, 2015;10(3):e0120261.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  12. Decidual neutrophil infiltration is not required for preterm birth in a mouse model of infection-induced preterm labor.
    Authors: Rinaldi S, Catalano R, Wade J, Rossi A, Norman J
    J Immunol, 2014;192(5):2315-25.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  13. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
    Authors: Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C
    J Immunol, 2014;192(11):5332-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Isotype control
  14. Crosstalk between mitochondrial ROS and depolarization in the potentiation of TRAIL-induced apoptosis in human tumor cells.
    Authors: Suzuki-Karasaki M, Ochiai T, Suzuki-Karasaki Y
    Int J Oncol, 2014;44(2):616-28.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Ctrl
  15. Overexpression of IL-17RC associated with ocular sarcoidosis.
    Authors: Wu W, Jin M, Wang Y, Liu B, Shen D, Chen P, Hannes S, Li Z, Hirani S, Jawad S, Sen H, Chan C, Nussenblatt R, Wei L
    J Transl Med, 2014;12(0):152.
    Species: Human
    Sample Type: Whole Blood
    Application: Flow control
  16. A role for PVRL4-driven cell-cell interactions in tumorigenesis.
    Authors: Pavlova N, Pallasch C, Elia A, Braun C, Westbrook T, Hemann M, Elledge S
    Elife, 2013;2(0):e00358.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  17. Interleukin-9 is required for allergic airway inflammation mediated by the cytokine TSLP.
    Authors: Yao W, Zhang Y, Jabeen R, Nguyen E, Wilkes D, Tepper R, Kaplan M, Zhou B
    Immunity, 2013;38(2):360-72.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  18. IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
    Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
    PLoS ONE, 2012;7(7):e40620.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  19. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
    Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P
    PLoS ONE, 2012;7(2):e31959.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  20. Inhibitory receptors are expressed by Trypanosoma cruzi-specific effector T cells and in hearts of subjects with chronic Chagas disease.
    Authors: Arguello RJ, Albareda MC, Alvarez MG, Bertocchi G, Armenti AH, Vigliano C, Meckert PC, Tarleton RL, Laucella SA
    PLoS ONE, 2012;7(5):e35966.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  21. MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production.
    Authors: Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, Liu W, Chen J, Xu L, Chen L, Duan Y
    PLoS ONE, 2012;7(12):e50589.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  22. Inhibition of transcription factor specificity protein 1 alters the gene expression profile of keratinocytes leading to upregulation of kallikrein-related peptidases and thymic stromal lymphopoietin.
    Authors: Bin L, Kim BE, Hall CF
    J. Invest. Dermatol., 2011;131(11):2213-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  23. Natural killer cell killing of acute myelogenous leukemia and acute lymphoblastic leukemia blasts by killer cell immunoglobulin-like receptor-negative natural killer cells after NKG2A and LIR-1 blockade.
    Authors: Godal R, Bachanova V, Gleason M
    Biol. Blood Marrow Transplant., 2010;16(5):612-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  24. pVHL co-ordinately regulates CXCR4/CXCL12 and MMP2/MMP9 expression in human clear-cell renal cell carcinoma.
    Authors: Struckmann K, Mertz K, Steu S, Storz M, Staller P, Krek W, Schraml P, Moch H
    J. Pathol., 2008;214(4):464-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  25. BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
    Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
    Blood, 2008;112(8):3154-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  26. Expression and regulation of CCR1 by airway smooth muscle cells in asthma.
    Authors: Joubert P, Lajoie-Kadoch S, Welman M, Dragon S, Letuvee S, Tolloczko B, Halayko AJ, Gounni AS, Maghni K, Hamid Q
    J. Immunol., 2008;180(2):1268-75.
    Species: Human
    Sample Type: Whole Tissue
    Application: Ctrl
  27. Induction of host chemotactic response by Encephalitozoon spp.
    Authors: Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H
    Infect. Immun., 2007;75(4):1619-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  28. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007;110(13):4319-30.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Ctrl
  29. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007;178(7):4184-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  30. Antibody-based inhibition of DKK1 suppresses tumor-induced bone resorption and multiple myeloma growth in vivo.
    Authors: Sheehan JJ, Yaccoby S, Ling W, Zhou C, Gravanis I, Zhan F, Walker R, Rogove AD, Wu YP, Barlogie B, Shaughnessy JD, Bogenhagen DF, Tsirka SE
    Blood, 2007;109(5):2106-11.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  31. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006;119(10):2313-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  32. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
Expand to show all 32 Citations
Isotype Controls
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Mouse IgG2B PE-conjugated Antibody

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Mouse IgG2B APC-conjugated Antibody

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Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

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Mouse IgG2B Isotype Control

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Mouse IgG2B Fluorescein‑conjugated Control Antibody

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Mouse IgG2B Isotype Control

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Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control

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Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control

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Mouse IgG2B PerCP-conjugated Isotype Control

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Mouse IgG2B Biotinylated Isotype Control

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Mouse IgG2B Alexa Fluor® 405-conjugated Isotype Control

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Mouse IgG2B Alexa Fluor® 750-conjugated Isotype Control

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Mouse IgG2B Alexa Fluor® 594-conjugated Isotype Control

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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse IgG2B Isotype Control MAB004
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 Flow Human Anonymous 05/02/2016
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Flow Cytometry Mouse IgG2B Isotype Control MAB004
Flow Cytometry: Mouse IgG2B Isotype Control [MAB004]

Summary

ApplicationFlow Cytometry
Sample TestedBlood-derived lymphocytes
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThe flow cytometry histogram shows the Mouse IgG2B Isotype Control (red) and TLR-2 (blue). The lymphocytes were gated on based on their FSC/SSC characteristics.

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