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Mouse IgG2B Isotype Control

R&D Systems | Catalog # MAB004

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Key Product Details

Species Reactivity

Mouse

Applications

Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 20116
View all IgG2B Isotype Controls »

Product Specifications

Immunogen

KLH

Specificity

Selected for use as mouse IgG2B isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT II (rhMIP-1 beta ), rhBLC/BCA-1, rmBLC, rhBRAK, rmBRAK, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC‑2 beta, rrCINC-3, rhCK beta 8-1, rmCRG-2, rmCTACK, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin 2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmI-TAC, rmJE, rmKC, rhLeukotactin-1, rrLIX, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma, rhMIP-1 delta, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, recombinant viral (rv) MIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF‑1 alpha, rmSDF‑1 alpha, rhSDF‑1 beta, rhTarc, rmTarc, rmTCA-3, rhTeck, rmTeck, rhVIC, or rmVIC.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Mouse IgG2B Isotype Control

Detection of Mouse IgG Isotype Control by Flow Cytometry

Detection of Mouse IgG Control by Flow Cytometry

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ITFG1 Monoclonal Antibody (Catalog # MAB89001, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog F0101B).

Applications for Mouse IgG2B Isotype Control

Application
Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.

Reviewed Applications

Read 8 reviews rated 4.6 using MAB004 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Additional IgG2B Products

Product Documents for Mouse IgG2B Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse IgG2B Isotype Control

For research use only

Citations for Mouse IgG2B Isotype Control

Customer Reviews for Mouse IgG2B Isotype Control (8)

4.6 out of 5
8 Customer Ratings
5 Stars
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4 Stars
38%
3 Stars
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1 Stars
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Customer Images

Showing  1 - 5 of 8 reviews Showing All
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  • Mouse IgG2B Isotype Control
    Name: Lu Bai
    Application: Flow Cytometry
    Sample Tested: Dendritic cells
    Species: Human
    Verified Customer | Posted 10/11/2022
  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 08/18/2021
    Mouse IgG2B Isotype Control MAB004
  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Myotubes
    Species: horse
    Verified Customer | Posted 12/04/2020
  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: MDAMB231
    Species: Human
    Verified Customer | Posted 11/16/2018
    Mouse IgG2B Isotype Control MAB004
  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Verified Customer | Posted 09/24/2018
    Mouse IgG2B Isotype Control MAB004
  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human cell line
    Species: Human
    Verified Customer | Posted 03/15/2018
    Mouse IgG2B Isotype Control MAB004
  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Verified Customer | Posted 03/09/2018
    Mouse IgG2B Isotype Control MAB004
  • Mouse IgG2B Isotype Control
    Name: Ahmad Al-Attar
    Application: Flow Cytometry
    Sample Tested: Blood-derived lymphocytes
    Species: Human
    Verified Customer | Posted 05/02/2016
    The flow cytometry histogram shows the Mouse IgG2B Isotype Control (red) and TLR-2 (blue). The lymphocytes were gated on based on their FSC/SSC characteristics.
    Mouse IgG2B Isotype Control MAB004

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FAQs

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