hh Mouse IGSF4A/SynCAM1 Biotinylated Antibody BAF1459: R&D Systems

Mouse IGSF4A/SynCAM1 Biotinylated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IGSF4A/SynCAM1 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse IGSF8 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IGSF4A/SynCAM1 isoform c
    Ile42-Asp376
    Accession # NP_061240
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IGSF4A/SynCAM1 (Catalog # 1459-S4)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IGSF4A/SynCAM1 in P815 Mouse Cell Line by Flow Cytometry. P815 mouse masto­cytoma cell line was stained with Goat Anti-Mouse IGSF4A/SynCAM1 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF1459, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGSF4A/SynCAM1

IGSF4A is a 75 kDa immunoglobulin superfamily protein that mediates calcium-independent adhesive interactions, either homophilic or heterophilic with CRTAM (class I-restricted T cell-associated molecule), Necl-1 (SynCAM3) or Nectin-3 (1‑4). Alternate names for IGSF4A reflect its activities. As TSLC-1 (tumor suppressor in lung cancer-1), it is downregulated in non-small cell lung cancer (5). This may allow evasion of antitumor responses, since TSLC-1 interaction with CRTAM on activated NK and CD8+ T cells stimulates cytotoxicity and upregulates IFN-gamma production (3, 6). As Necl-2 (nectin-like 2), it is expressed on T zone-restricted BDCA3+ dendritic cells, again interacting with CRTAM (3). As SynCAM-1 (synaptic cell adhesion molecule-1) it binds homotypically across synaptic clefts and, like neuroligin/neurexin adhesion pairs, promotes formation of neural cell synapses (7, 8). Expression on mast cells allows attachment to neurites and fibroblasts (9, 10). As SgIGSF (spermatogenic IgSF), expression on developing sperm allows adhesion to an unknown partner on Sertoli cells that is critical for normal development (11, 12). Mouse IGSF4A cDNA encodes 445 amino acids including a signal peptide, three Ig-like domains (a V-type, a divergent C1-type and an I-type), a transmembrane domain and a cytoplasmic domain with protein 4.1 and PDZ domain binding sites. These sites allow connections to adaptor molecules and are critical in synaptogenesis (7, 8). The outer Ig-like domain most likely mediates adhesion, with altered affinity for splice variants that have either shorter spacing of this domain from the plasma membrane or varied glycosylation (1, 6). The six reported splice variants include a prominently expressed soluble form of about 45 kDa that inhibits adhesion (13). Mouse IGSF4A ECD shares 99%, 96%, 98% and 88% amino acid identity with rat, human, canine, and bovine IGSF4A, respectively.

  • References:
    1. Biederer, T. et al. (2006) Genomics 87:139.
    2. Wakayama, T. et al. (2001) Mol. Reprod. Dev. 60:158.
    3. Galibert, L. et al. (2005) J. Biol. Chem. 280:21955.
    4. Shingai, T. et al. (2003) J. Biol. Chem. 278:35421.
    5. Kuramochi, M. et al. (2002) Nat. Genet. 27:427.
    6. Boles, K.S. et al. (2005) Blood 106:779.
    7. Biederer, T. et al. (2002) Science 297:1525.
    8. Sara, Y. et al. (2005) J. Neurosci. 25:260.
    9. Furuno, T. et al. (2005) J. Immunol. 174:6934.
    10. Ito, A. et al. (2003) Blood 101:2601.
    11. Wakayama, T. et al. (2001) Mol. Reprod. Dev. 60:158.
    12. Fujita, E. et al. (2006) Mol. Cell. Biol. 26:718.
    13. Koma, Y. et al. (2004) Oncogene 23:5687.
  • Long Name:
    Immunoglobulin Superfamily, Member 4
  • Entrez Gene IDs:
    23705 (Human); 16151 (Mouse)
  • Alternate Names:
    BL2; Cadm1; cell adhesion molecule 1; IgSF4; IGSF4A; IGSF4ADKFZp686F1789; IGSF4immunoglobulin superfamily, member 4D variant 2; Immunoglobulin superfamily member 4; immunoglobulin superfamily, member 4; Necl-2; NECL2MGC149785; nectin-like 2; Nectin-like protein 2; RA175; sgIGSF; Spermatogenic immunoglobulin superfamily; ST17; sTSLC-1; Synaptic cell adhesion molecule; SynCAM; SynCAM1; SYNCAMMGC51880; TSLC1; TSLC-1; TSLC1/Nectin-like 2/IGSF4; Tumor suppressor in lung cancer 1SYNCAM1
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