Mouse IL-12 Biotinylated Antibody
Mouse IL-12 Biotinylated Antibody Summary
p40 (Met23-Ser335, P43432) p35 (Arg23-Ala215, NP_032377)
Applications
Mouse IL-12 p70 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12
Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.
Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems' recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 ‑ G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.
Product Datasheets
Citations for Mouse IL-12 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells
Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon
J. Allergy Clin. Immunol., 2017;139(5):1608-1620.e3.
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Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Assay Development -
IL-35 stimulation of CD39+ regulatory T cells confers protection against collagen II-induced arthritis via the production of IL-10.
Authors: Kochetkova I, Golden S, Holderness K
J. Immunol., 2010;184(12):7144-53.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Western Blot -
Expression of bioactive single-chain murine IL-12 in transgenic plants.
Authors: Liu J, Dolan MC, Reidy M, Cramer CL
J. Interferon Cytokine Res., 2009;28(0):381-92.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development -
Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
J Ethnopharmacol, 2006;106(3):418-24.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
J. Immunol., 2006;177(12):8650-7.
Species: Mouse
Sample Types: Peritoneal Lavage
Applications: ELISA Development -
Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
Am. J. Pathol., 2006;168(6):1940-50.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development -
Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005;302(1):172-81.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development -
17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
Blood, 2004;104(5):1404-10.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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This antibody was paired with MAB419 to create an ELISA for mouse IL-12.
