Mouse IL-12/IL-23 p40 Antibody

Catalog # Availability Size / Price Qty
MAB499-SP
MAB499-100
MAB499-500
Product Details
Citations (3)
FAQs
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Mouse IL-12/IL-23 p40 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-12 p40 in ELISAs. Polymorphisms exist in the mouse IL-12 p40 sequence. This antibody recognizes an allelic epitope found in the p40 subunit of several mouse strains including BALB/c, C57BL/6, CBA, C3H, DBA/1, NOR, I29/J, and A.SW. This antibody does not recognize the allelic variant that occurs in strains AKR, DBA/2, NOD, NZB, NZC, NZO, NZW, or SJL (1). In ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse (rm) IL-23, 30% cross-reactivity with rmIL-12 p70, and no cross-reactivity with recombinant human (rh) IL-12 p70 or rhIL-12 p40.
Source
Monoclonal Rat IgG2B Clone # 30517
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑12 p40
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Mouse IL-12/IL-23 p40 Allele-specific Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑12/IL‑23 p40 Biotinylated Antibody (Catalog # BAF499)

Standard: Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein (Catalog # 499-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-23 p40

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells.

IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells.

The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined.

References
  1. Ymer, S. et al. (2002) Genes and Immunity 3:151.
Long Name
Interleukin 12/Interleukin 23 p40
Entrez Gene IDs
3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)
Alternate Names
CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40

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Citations for Mouse IL-12/IL-23 p40 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Non-canonical interleukin 23 receptor complex assembly: p40 protein recruits interleukin 12 receptor beta1 via site II and induces p19/interleukin 23 receptor interaction via site III.
    Authors: Schroder J, Moll J, Baran P, Grotzinger J, Scheller J, Floss D
    J Biol Chem, 2014-11-04;290(1):359-70.
    Species: Mouse, Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. IL-23 is required for neutrophil homeostasis in normal and neutrophilic mice.
    Authors: Smith E, Zarbock A, Stark MA, Burcin TL, Bruce AC, Foley P, Ley K
    J. Immunol., 2007-12-15;179(12):8274-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
    Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
    Am. J. Pathol., 2002-04-01;160(4):1353-60.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

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