Mouse IL-13 Antibody

(11 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-13 in ELISAs and Western blots. In ELISAs, less than 0.1% cross-reactivity with recombinant human IL‑13 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 38213
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse IL-13
    Accession # P20109
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse IL‑13 (Catalog # 413-ML)
    • Mouse IL-13 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse IL‑13 Antibody (Catalog # MAB413)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse IL‑13 Biotinylated Antibody (Catalog # BAF413)
  • ELISA Standard
     
    Recombinant Mouse IL-13 Protein (Catalog # 413-ML)
  • Neutralization
    Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.3-0.8 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑13.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. Recombinant Mouse IL‑13 (Catalog # 413-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑13 Monoclonal Antibody (Catalog # MAB413). The ND50 is typically 0.3-0.8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

  • References:
    1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
    2. Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
    3. Brown, K.D. et al. (1989) J. Immunol. 142:679.
    4. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
    5. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
    6. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
    7. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
    8. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
    9. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
    10. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
    11. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
    12. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
    13. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
    14. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
    15. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
    16. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
    17. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
  • Long Name:
    Interleukin 13
  • Entrez Gene IDs:
    3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
  • Alternate Names:
    ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Macrophage migration inhibitory factor deficiency enhances immune response to Nippostrongylus brasiliensis
    Authors: SR Damle, RK Martin, JV Cross, DH Conrad
    Mucosal Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  2. RSV vaccine-enhanced disease is orchestrated by the combined actions of distinct CD4 T cell subsets.
    Authors: Knudson C, Hartwig S, Meyerholz D, Varga S
    PLoS Pathog, 2015;11(3):e1004757.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  3. CD8(+) cells regulate the T helper-17 response in an experimental murine model of Sjogren syndrome.
    Authors: Zhang X, Schaumburg C, Coursey T, Siemasko K, Volpe E, Gandhi N, Li D, Niederkorn J, Stern M, Pflugfelder S, de Paiva C
    Mucosal Immunol, 2014;7(2):417-27.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ELISA Capture
  4. Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
    Authors: Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM
    J. Immunol., 2010;184(8):4159-69.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  5. Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development.
    Authors: Mosconi E, Rekima A, Seitz-Polski B
    Mucosal Immunol, 2010;3(5):461-74.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  6. IL-6 is required for airway mucus production induced by inhaled fungal allergens.
    Authors: Neveu WA, Allard JB, Dienz O, Wargo MJ, Ciliberto G, Whittaker LA, Rincon M
    J. Immunol., 2009;183(3):1732-8.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  7. FTY720 ameliorates oxazolone colitis in mice by directly affecting T helper type 2 functions.
    Authors: Daniel C, Sartory NA, Zahn N
    Mol. Immunol., 2007;44(13):3305-16.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  8. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006;168(6):1940-50.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  9. Loss of pro-apoptotic Bim promotes accumulation of pulmonary T lymphocytes and enhances allergen-induced goblet cell metaplasia.
    Authors: Pierce J, Rir-Sima-Ah J, Estrada I, Wilder J, Strasser A, Tesfaigzi Y
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(5):L862-70.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  10. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines.
    Authors: Schmitz J, Owyang A, Oldham E, Song Y, Murphy E, McClanahan TK, Zurawski G, Moshrefi M, Qin J, Li X, Gorman DM, Bazan JF, Kastelein RA
    Immunity, 2005;23(5):479-90.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
  11. Persistence of bronchopulmonary hyper-reactivity and eosinophilic lung inflammation after anti-IL-5 or -IL-13 treatment in allergic BALB/c and IL-4Ralpha knockout mice.
    Authors: Proust B, Nahori MA, Ruffie C, Lefort J, Vargaftig BB
    Clin. Exp. Allergy, 2003;33(1):119-31.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Expand to show all 11 Citations
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