Mouse IL-13 Culture Media Quantikine ELISA

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PM1300CB
SM1300CB
M1300CB
Mouse IL-13 Standard Curve
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Product Details
Procedure
Citations (108)
FAQs
Supplemental Products
Reviews (3)

Mouse IL-13 Culture Media Quantikine ELISA Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL)
Sensitivity
1.5 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates)
Specificity
Natural and recombinant mouse IL-13
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-13 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-13 levels in cell culture supernates. It contains E. coli-expressed recombinant mouse IL-13 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-13 accurately. Results obtained using natural mouse IL-13 showed dose curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-13.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 33.9 100 432 31.6 93.4 405
Standard Deviation 1.1 2.7 11.2 2.5 7.2 17.7
CV% 3.2 2.7 2.6 7.9 7.7 4.4

Recovery

The recovery of mouse IL-13 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=13) 99 89-119

Linearity

To assess the linearity of the assay, samples containing and/or spiked with mouse IL-13 were diluted with Calibrator Diluent and assayed.
Mouse IL-13 ELISA Linearity

Data Examples

Mouse IL-13 Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-13

IL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.

Long Name:
Interleukin 13
Entrez Gene IDs:
3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
Alternate Names:
ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse IL-13 Culture Media Quantikine ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

108 Citations: Showing 1 - 10
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  1. Andrographolide prevented toluene diisocyanate-induced occupational asthma and aberrant airway E-cadherin distribution via p38 MAPK-dependent Nrf2 induction
    Authors: I Sulaiman, K Tan, N Mohtarrudi, JC Woei Lim, J Stanslas
    Pulm Pharmacol Ther, 2018;0(0):.
    Species: Mouse
    Sample Types: BALF
  2. Multi-Walled Carbon Nanotubes Augment Allergic Airway Eosinophilic Inflammation by Promoting Cysteinyl Leukotriene Production
    Authors: S Carvalho, M Ferrini, L Herritt, A Holian, Z Jaffar, K Roberts
    Front Pharmacol, 2018;9(0):585.
    Species: Mouse
    Sample Types: BALF
  3. Effects of the Th2-dominant milieu on allergic responses in Der f 1-activated mouse basophils and mast cells
    Authors: MH Yi, HP Kim, KY Jeong, JY Kim, IY Lee, TS Yong
    Sci Rep, 2018;8(1):7706.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
    Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Effect of a retinoid X receptor partial agonist on airway inflammation and hyperresponsiveness in a murine model of asthma
    Authors: U Fujii, N Miyahara, A Taniguchi, N Oda, D Morichika, E Murakami, H Nakayama, K Waseda, M Kataoka, H Kakuta, M Tanimoto, A Kanehiro
    Respir. Res, 2017;18(1):23.
    Species: Mouse
    Sample Types: BALF
  6. Pulmonary ORMDL3 is critical for induction of Alternaria-induced allergic airways disease
    Authors: S Löser, LG Gregory, Y Zhang, K Schaefer, SA Walker, J Buckley, L Denney, CH Dean, WOC Cookson, MF Moffatt, CM Lloyd
    J. Allergy Clin. Immunol., 2017;139(5):1496-1507.e3.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine
    Authors: YT Lee, CT Wu, HL Sun, JL Ko, KH Lue
    J Microbiol Immunol Infect, 2017;0(0):.
    Species: Mouse
    Sample Types: BALF
  8. Increased mitochondrial arginine metabolism supports bioenergetics in asthma
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Types: BALF
  9. PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen
    Authors: Bryan Simons
    J. Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: BALF
  10. NLRP3 inflammasome has a protective effect against oxazolone-induced colitis: a possible role in ulcerative colitis
    Sci Rep, 2016;6(0):39075.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Lyn kinase represses mucus hypersecretion by regulating IL-13-induced endoplasmic reticulum stress in asthma
    EBioMedicine, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. NLRP3 inflammasome has a protective effect against oxazolone-induced colitis: a possible role in ulcerative colitis
    Sci Rep, 2016;6(0):39075.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. G-protein-coupled estrogen receptor agonist suppresses airway inflammation in a mouse model of asthma through IL-10.
    Authors: Itoga M, Konno Y, Moritoki Y, Saito Y, Ito W, Tamaki M, Kobayashi Y, Kayaba H, Kikuchi Y, Chihara J, Takeda M, Ueki S, Hirokawa M
    PLoS ONE, 2015;10(3):e0123210.
    Species: Mouse
    Sample Types: BALF
  14. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
    Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
    Nat Commun, 2015;6(0):6224.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. PU.1 Suppresses Th2 Cytokine Expression via Silencing of GATA3 Transcription in Dendritic Cells.
    Authors: Yashiro T, Kubo M, Ogawa H, Okumura K, Nishiyama C
    PLoS ONE, 2015;10(9):e0137699.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Impact of ozone exposure on the response to glucocorticoid in a mouse model of asthma: involvements of p38 MAPK and MKP-1.
    Authors: Bao, Aihua, Li, Feng, Zhang, Min, Chen, Yuqing, Zhang, Pengyu, Zhou, Xin
    Respir Res, 2014;15(0):126.
    Species: Mouse
    Sample Types: BALF
  17. Leishmania donovani targets tumor necrosis factor receptor-associated factor (TRAF) 3 for impairing TLR4-mediated host response.
    Authors: Gupta P, Giri J, Srivastav S, Chande A, Mukhopadhyaya R, Das P, Ukil A
    FASEB J, 2014;28(4):1756-68.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Genetic vaccine for respiratory syncytial virus provides protection without disease potentiation.
    Authors: Johnson T, Rangel D, Graham B, Brough D, Gall J
    Mol Ther, 2014;22(1):196-205.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. STAT4 deficiency fails to induce lung Th2 or Th17 immunity following primary or secondary respiratory syncytial virus (RSV) challenge but enhances the lung RSV-specific CD8+ T cell immune response to secondary challenge.
    Authors: Dulek D, Newcomb D, Toki S, Goliniewska K, Cephus J, Reiss S, Bates J, Crowe J, Boyd K, Moore M, Zhou W, Peebles R
    J Virol, 2014;88(17):9655-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. Axl receptor blockade ameliorates pulmonary pathology resulting from primary viral infection and viral exacerbation of asthma.
    Authors: Shibata T, Habiel D, Coelho A, Kunkel S, Lukacs N, Hogaboam C
    J Immunol, 2014;192(8):3569-81.
    Species: Mouse
    Sample Types: Tissue Homogenates
  21. Antibody to mCLCA3 suppresses symptoms in a mouse model of asthma.
    Authors: Song L, Liu D, Wu C, Wu S, Yang J, Ren F, Li Y
    PLoS ONE, 2013;8(12):e82367.
    Species: Mouse
    Sample Types: BALF
  22. Induction of the specific allergic immune response is independent of proteases from the fungus Alternaria alternata.
    Authors: Denis O, Vincent M, Havaux X, De Prins S, Treutens G, Huygen K
    Eur J Immunol, 2013;43(4):907-17.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Monocyte-derived dendritic cell recruitment and allergic T(H)2 responses after exposure to diesel particles are CCR2 dependent.
    Authors: Provoost S, Maes T, Joos GF, Tournoy KG
    J. Allergy Clin. Immunol., 2012;129(2):483-91.
    Species: Mouse
    Sample Types: BALF
  24. Apoptotic cells suppress mast cell inflammatory responses via the CD300a immunoreceptor.
    J. Exp. Med., 2012;209(8):1493-503.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. TLR-mediated distinct IFN-gamma/IL-10 pattern induces protective immunity against murine visceral leishmaniasis.
    Eur. J. Immunol., 2012;42(8):2087-99.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
    Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
    Am. J. Pathol., 2012;179(1):302-14.
    Species: Mouse
    Sample Types: Tissue Homogenates
  27. Endogenous IL-33 enhances Th2 cytokine production and T-cell responses during allergic airway inflammation.
    Authors: Louten J, Rankin AL, Li Y
    Int. Immunol., 2011;23(5):307-15.
    Species: Mouse
    Sample Types: BALF
  28. The tumor necrosis factor family member LIGHT is a target for asthmatic airway remodeling.
    Authors: Doherty TA, Soroosh P, Khorram N, Fukuyama S, Rosenthal P, Cho JY, Norris PS, Choi H, Scheu S, Pfeffer K, Zuraw BL, Ware CF, Broide DH, Croft M
    Nat. Med., 2011;17(5):596-603.
    Species: Mouse
    Sample Types: Tissue Homogenates
  29. IL-33-activated dendritic cells are critical for allergic airway inflammation.
    Authors: Besnard AG, Togbe D, Guillou N, Erard F, Quesniaux V, Ryffel B
    Eur. J. Immunol., 2011;41(6):1675-86.
    Species: Mouse
    Sample Types: BALF
  30. IL-22 Is Produced by Innate Lymphoid Cells and Limits Inflammation in Allergic Airway Disease.
    Authors: Taube C, Tertilt C, Gyulveszi G, Dehzad N, Kreymborg K, Schneeweiss K, Michel E, Reuter S, Renauld JC, Arnold-Schild D, Schild H, Buhl R, Becher B
    PLoS ONE, 2011;6(7):e21799.
    Species: Mouse
    Sample Types: BALF
  31. Reciprocal expression of IL-25 and IL-17A is important for allergic airways hyperreactivity.
    Authors: Barlow JL, Flynn RJ, Ballantyne SJ, McKenzie AN
    Clin. Exp. Allergy, 2011;41(10):1447-55.
    Species: Mouse
    Sample Types: BALF
  32. Over-expression of the LTC4 synthase gene in mice reproduces human aspirin-induced asthma.
    Authors: Hirata H, Arima M, Fukushima Y, Honda K, Sugiyama K, Tokuhisa T, Fukuda T
    Clin. Exp. Allergy, 2011;41(8):1133-42.
    Species: Mouse
    Sample Types: BALF
  33. Interleukin-13 promotes susceptibility to chlamydial infection of the respiratory and genital tracts.
    Authors: Asquith KL, Horvat JC, Kaiko GE
    PLoS Pathog., 2011;7(5):e1001339.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  34. IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs.
    Authors: Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H
    J. Immunol., 2011;188(3):1503-13.
    Species: Mouse
    Sample Types: BALF
  35. The TLR7 ligand 9-benzyl-2-butoxy-8-hydroxy adenine inhibits IL-17 response by eliciting IL-10 and IL-10-inducing cytokines.
    Authors: Vultaggio A, Nencini F, Pratesi S, Maggi L, Guarna A, Annunziato F, Romagnani S, Parronchi P, Maggi E
    J. Immunol., 2011;186(8):4707-15.
    Species: Mouse
    Sample Types: Serum
  36. Control of mouse graft-versus-host disease following allogeneic bone marrow transplantation by blocking the CD28/B7 signaling pathway with lentiviral vector-mediated RNA interference.
    Authors: Sang W, Zhou C, Cheng N, Li Z, Zeng L, Xu K
    Immunol. Lett., 2011;136(2):194-202.
    Species: Mouse
    Sample Types: Plasma
  37. Mucosal vaccination using claudin-4-targeting.
    Authors: Kakutani H, Kondoh M, Fukasaka M, Suzuki H, Hamakubo T, Yagi K
    Biomaterials, 2010;31(20):5463-71.
    Species: Mouse
    Sample Types: Serum
  38. Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice.
    Authors: Alm AS, Li K, Yang D, Andersson R, Lu Y, Wang X
    Cytokine, 2010;49(3):256-63.
    Species: Mouse
    Sample Types: BALF
  39. Anti-Asthma Simplified Herbal Medicine Intervention-induced long-lasting tolerance to allergen exposure in an asthma model is interferon-gamma, but not transforming growth factor-beta dependent.
    Authors: Srivastava K, Zhang T, Yang N, Sampson H, Li XM
    Clin. Exp. Allergy, 2010;40(11):1678-88.
    Species: Mouse
    Sample Types: BALF
  40. Nuocytes represent a new innate effector leukocyte that mediates type-2 immunity.
    Authors: Neill DR, Wong SH, Bellosi A, Flynn RJ, Daly M, Langford TK, Bucks C, Kane CM, Fallon PG, Pannell R, Jolin HE, McKenzie AN
    Nature, 2010;464(7293):1367-70.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  41. Epithelial reticulon 4B (Nogo-B) is an endogenous regulator of Th2-driven lung inflammation.
    Authors: Wright PL, Yu J, Di YP
    J. Exp. Med., 2010;207(12):2595-607.
    Species: Mouse
    Sample Types: BALF
  42. Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling.
    Authors: Graham BB, Mentink-Kane MM, El-Haddad H
    Am. J. Pathol., 2010;177(3):1549-61.
    Species: Mouse
    Sample Types: Tissue Homogenates
  43. House dust mite induces direct airway inflammation in vivo: implications for future disease therapy?
    Authors: De Alba J, Raemdonck K, Dekkak A, Collins M, Wong S, Nials AT, Knowles RG, Belvisi MG, Birrell MA
    Eur. Respir. J., 2010;35(6):1377-87.
    Species: Mouse
    Sample Types: BALF
  44. Modified adenine (9-benzyl-2-butoxy-8-hydroxyadenine) redirects Th2-mediated murine lung inflammation by triggering TLR7.
    Authors: Vultaggio A, Nencini F, Fitch PM, Fili L, Maggi L, Fanti P, deVries A, Beccastrini E, Palandri F, Manuelli C, Bani D, Giudizi MG, Guarna A, Annunziato F, Romagnani S, Maggi E, Howie SE, Parronchi P
    J. Immunol., 2009;182(2):880-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  45. A severe deficiency of coagulation factor VIIa results in attenuation of the asthmatic response in mice.
    Authors: Shinagawa K, Ploplis VA, Castellino FJ
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L763-70.
    Species: Mouse
    Sample Types: BALF
  46. TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity.
    Authors: Johnson TR, Rao S, Seder RA, Chen M, Graham BS
    Vaccine, 2009;27(23):3045-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  47. Intranasal challenge with increasing ovalbumin doses differently affects airway hyperresponsiveness and inflammatory cell accumulation in mouse model of asthma.
    Authors: Bosnjak B, Ivetic Tkalcevic V, Duric K, Belamaric D, Cuzic S, Ferencic Z, Brajsa K, Glojnaric I, Antolovic R, Hrvacic B
    Inflamm. Res., 2009;58(11):773-81.
    Species: Mouse
    Sample Types: BALF
  48. The adaptor protein CIKS/Act1 is essential for IL-25-mediated allergic airway inflammation.
    Authors: Claudio E, Sonder SU, Saret S, Carvalho G, Ramalingam TR, Wynn TA, Chariot A, Garcia-Perganeda A, Leonardi A, Paun A, Chen A, Ren NY, Wang H, Siebenlist U
    J. Immunol., 2009;182(3):1617-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  49. Eosinophil viability is increased by acidic pH in a cAMP- and GPR65-dependent manner.
    Authors: Kottyan LC, Collier AR, Cao KH, Niese KA, Hedgebeth M, Radu CG, Witte ON, Khurana Hershey GK, Rothenberg ME, Zimmermann N
    Blood, 2009;114(13):2774-82.
    Species: Mouse
    Sample Types: Cell Lysates
  50. A TLR2 agonist in German cockroach frass activates MMP-9 release and is protective against allergic inflammation in mice.
    Authors: Page K, Ledford JR, Zhou P, Wills-Karp M
    J. Immunol., 2009;183(5):3400-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
  51. Temporal changes in myeloid cells in the cervix during pregnancy and parturition.
    Authors: Timmons BC, Fairhurst AM, Mahendroo MS
    J. Immunol., 2009;182(5):2700-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
  52. Effects of a leukotriene B4 receptor antagonist on bleomycin-induced pulmonary fibrosis.
    Authors: Izumo T, Kondo M, Nagai A
    Eur. Respir. J., 2009;34(6):1444-51.
    Species: Mouse
    Sample Types: BALF
  53. Mouse models of asthma: a comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness, inflammation, and cytokine production.
    Authors: Gueders MM, Paulissen G, Crahay C, Quesada-Calvo F, Hacha J, Van Hove C, Tournoy K, Louis R, Foidart JM, Noel A, Cataldo DD
    Inflamm. Res., 2009;58(12):845-54.
    Species: Mouse
    Sample Types: BALF
  54. Exaggerated IL-17 response to epicutaneous sensitization mediates airway inflammation in the absence of IL-4 and IL-13.
    Authors: He R, Kim HY, Yoon J, Oyoshi MK, MacGinnitie A, Goya S, Freyschmidt EJ, Bryce P, McKenzie AN, Umetsu DT, Oettgen HC, Geha RS
    J. Allergy Clin. Immunol., 2009;124(4):761-70.e1.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  55. Osteopontin deficiency protects against airway remodeling and hyperresponsiveness in chronic asthma.
    Authors: Simoes DC, Xanthou G, Petrochilou K, Panoutsakopoulou V, Roussos C, Gratziou C
    Am. J. Respir. Crit. Care Med., 2009;179(10):894-902.
    Species: Mouse
    Sample Types: Tissue Homogenates
  56. Peroxisome proliferator-activated receptor gamma agonist down-regulates IL-17 expression in a murine model of allergic airway inflammation.
    Authors: Park SJ, Lee KS, Kim SR, Min KH, Choe YH, Moon H, Chae HJ, Yoo WH, Lee YC
    J. Immunol., 2009;183(5):3259-67.
    Species: Mouse
    Sample Types: BALF
  57. Complement C3a regulates late asthmatic response and airway hyperresponsiveness in mice.
    Authors: Mizutani N, Nabe T, Yoshino S
    J. Immunol., 2009;183(6):4039-46.
    Species: Mouse
    Sample Types: BALF
  58. Macrophage migration inhibitory factor homologs of anisakis simplex suppress Th2 response in allergic airway inflammation model via CD4+CD25+Foxp3+ T cell recruitment.
    Authors: Park SK, Cho MK, Park HK, Lee KH, Lee SJ, Choi SH, Ock MS, Jeong HJ, Lee MH, Yu HS
    J. Immunol., 2009;182(11):6907-14.
    Species: Mouse
    Sample Types: BALF
  59. Celastrol suppresses allergen-induced airway inflammation in a mouse allergic asthma model.
    Authors: Kim DY, Park JW, Jeoung D, Ro JY
    Eur. J. Pharmacol., 2009;612(1):98-105.
    Species: Mouse
    Sample Types: BALF
  60. Lovastatin inhibits antigen-induced airway eosinophilia without affecting the production of inflammatory mediators in mice.
    Authors: Chiba Y, Sato S, Misawa M
    Inflamm. Res., 2009;0(0):.
    Species: Mouse
    Sample Types: BALF
  61. Inhaled multiwalled carbon nanotubes potentiate airway fibrosis in murine allergic asthma.
    Authors: Ryman-Rasmussen JP, Tewksbury EW, Moss OR, Cesta MF, Wong BA, Bonner JC
    Am. J. Respir. Cell Mol. Biol., 2009;40(3):349-58.
    Species: Mouse
    Sample Types: BALF
  62. A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis.
    Authors: Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM
    Am. J. Pathol., 2008;172(5):1209-21.
    Species: Mouse
    Sample Types: Tissue Homogenates
  63. Ecto-5'-nucleotidase (CD73) -mediated extracellular adenosine production plays a critical role in hepatic fibrosis.
    Authors: Peng Z, Fernandez P, Wilder T, Yee H, Chiriboga L, Chan ES, Cronstein BN
    FASEB J., 2008;22(7):2263-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
  64. Pulmonary eosinophilia requires interleukin-5, eotaxin-1, and CD4+ T cells in mice immunized with respiratory syncytial virus G glycoprotein.
    Authors: Johnson TR, Rothenberg ME, Graham BS
    J. Leukoc. Biol., 2008;84(3):748-59.
    Species: Mouse
    Sample Types: Tissue Homogenates
  65. GS143, an IkappaB ubiquitination inhibitor, inhibits allergic airway inflammation in mice.
    Authors: Hirose K, Wakashin H, Oki M, Kagami S, Suto A, Ikeda K, Watanabe N, Iwamoto I, Furuichi Y, Nakajima H
    Biochem. Biophys. Res. Commun., 2008;374(3):507-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  66. IL-13 receptor alpha2 selectively inhibits IL-13-induced responses in the murine lung.
    Authors: Zheng T, Liu W, Oh SY, Zhu Z, Hu B, Homer RJ, Cohn L, Grusby MJ, Elias JA
    J. Immunol., 2008;180(1):522-9.
    Species: Mouse
    Sample Types: BALF
  67. Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
    Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
    J. Exp. Med., 2008;205(3):699-710.
    Species: Mouse
    Sample Types: BALF
  68. CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
    Authors: Kearley J, Robinson DS, Lloyd CM
    J. Allergy Clin. Immunol., 2008;122(3):617-24.e6.
    Species: Mouse
    Sample Types: BALF
  69. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
    Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
    Eur. J. Pharmacol., 2008;590(1):400-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
  70. Adenovirus IL-13-induced airway disease in mice: a corticosteroid-resistant model of severe asthma.
    Authors: Therien AG, Bernier V, Weicker S, Tawa P, Falgueyret JP, Mathieu MC, Honsberger J, Pomerleau V, Robichaud A, Stocco R, Dufresne L, Houshyar H, Lafleur J, Ramachandran C, O'Neill GP, Slipetz D, Tan CM
    Am. J. Respir. Cell Mol. Biol., 2008;39(1):26-35.
    Species: Mouse
    Sample Types: BALF
  71. CD43 regulates Th2 differentiation and inflammation.
    Authors: Cannon JL, Collins A, Mody PD, Balachandran D, Henriksen KJ, Smith CE, Tong J, Clay BS, Miller SD, Sperling AI
    J. Immunol., 2008;180(11):7385-93.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  72. Novel immunomodulatory properties of berbamine through selective down-regulation of STAT4 and action of IFN-gamma in experimental autoimmune encephalomyelitis.
    Authors: Ren Y, Lu L, Guo TB, Qiu J, Yang Y, Liu A, Zhang JZ
    J. Immunol., 2008;181(2):1491-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  73. Cyclooxygenase inhibition during allergic sensitization increases STAT6-independent primary and memory Th2 responses.
    Authors: Zhou W, Newcomb DC, Moore ML, Goleniewska K, O'Neal JF, Peebles RS
    J. Immunol., 2008;181(8):5360-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
  74. T-cell-expressed proprotein convertase furin is essential for maintenance of peripheral immune tolerance.
    Authors: Pesu M, Watford WT, Wei L, Xu L, Fuss I, Strober W, Andersson J, Shevach EM, Quezado M, Bouladoux N, Roebroek A, Belkaid Y, Creemers J, O'Shea JJ
    Nature, 2008;455(7210):246-50.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  75. Coexpression of TGF-beta1 and IL-10 enables regulatory T cells to completely suppress airway hyperreactivity.
    Authors: Presser K, Schwinge D, Wegmann M, Huber S, Schmitt S, Quaas A, Maxeiner JH, Finotto S, Lohse AW, Blessing M, Schramm C
    J. Immunol., 2008;181(11):7751-8.
    Species: Mouse
    Sample Types: BALF
  76. Reversion of immune tolerance in advanced malignancy: modulation of myeloid-derived suppressor cell development by blockade of stem-cell factor function.
    Authors: Pan PY, Wang GX, Yin B, Ozao J, Ku T, Divino CM, Chen SH
    Blood, 2007;111(1):219-28.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  77. FTY720 ameliorates oxazolone colitis in mice by directly affecting T helper type 2 functions.
    Authors: Daniel C, Sartory NA, Zahn N
    Mol. Immunol., 2007;44(13):3305-16.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  78. Prolonged inhaled allergen exposure can induce persistent tolerance.
    Authors: Van Hove CL, Maes T, Joos GF, Tournoy KG
    Am. J. Respir. Cell Mol. Biol., 2007;36(5):573-84.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  79. Critical role of the Fc receptor gamma-chain on APCs in the development of allergen-induced airway hyperresponsiveness and inflammation.
    Authors: Kitamura K, Takeda K, Koya T, Miyahara N, Kodama T, Dakhama A, Takai T, Hirano A, Tanimoto M, Harada M, Gelfand EW
    J. Immunol., 2007;178(1):480-8.
    Species: Mouse
    Sample Types: BALF
  80. Chronic intranasal administration of mould spores or extracts to unsensitized mice leads to lung allergic inflammation, hyper-reactivity and remodelling.
    Authors: Denis O, van den Brule S, Heymans J, Havaux X, Rochard C, Huaux F, Huygen K
    Immunology, 2007;122(2):268-78.
    Species: Mouse
    Sample Types: BALF
  81. B7-DC induced by IL-13 works as a feedback regulator in the effector phase of allergic asthma.
    Authors: Matsumoto K, Fukuyama S, Eguchi-Tsuda M, Nakano T, Matsumoto T, Matsumura M, Moriwaki A, Kan-o K, Wada Y, Yagita H, Shin T, Pardoll DM, Patcharee R, Azuma M, Nakanishi Y, Inoue H
    Biochem. Biophys. Res. Commun., 2007;365(1):170-5.
    Species: Mouse
    Sample Types: BALF
  82. IL-27 suppresses Th2 cell development and Th2 cytokines production from polarized Th2 cells: a novel therapeutic way for Th2-mediated allergic inflammation.
    Authors: Yoshimoto T, Yoshimoto T, Yasuda K, Mizuguchi J, Nakanishi K
    J. Immunol., 2007;179(7):4415-23.
    Species: Mouse
    Sample Types: Serum
  83. Protectin D1 is generated in asthma and dampens airway inflammation and hyperresponsiveness.
    Authors: Levy BD, Kohli P, Gotlinger K, Haworth O, Hong S, Kazani S, Israel E, Haley KJ, Serhan CN
    J. Immunol., 2007;178(1):496-502.
    Species: Mouse
    Sample Types: BALF
  84. The JAK-3 inhibitor CP-690550 is a potent anti-inflammatory agent in a murine model of pulmonary eosinophilia.
    Authors: Kudlacz E, Conklyn M, Andresen C, Whitney-Pickett C, Changelian P
    Eur. J. Pharmacol., 2007;582(1):154-61.
    Species: Mouse
    Sample Types: BALF
  85. Src homology 2 domain-containing inositol 5-phosphatase 1 deficiency leads to a spontaneous allergic inflammation in the murine lung.
    Authors: Oh SY, Zheng T, Bailey ML, Barber DL, Schroeder JT, Kim YK, Zhu Z
    J. Allergy Clin. Immunol., 2007;119(1):123-31.
    Species: Mouse
    Sample Types: BALF
  86. T-bet inhibits both TH2 cell-mediated eosinophil recruitment and TH17 cell-mediated neutrophil recruitment into the airways.
    Authors: Fujiwara M, Hirose K, Kagami S, Takatori H, Wakashin H, Tamachi T, Watanabe N, Saito Y, Iwamoto I, Nakajima H
    J. Allergy Clin. Immunol., 2007;119(3):662-70.
    Species: Mouse
    Sample Types: BALF
  87. Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia.
    Authors: Choo-Wing R, Nedrelow JH, Homer RJ, Elias JA, Bhandari V
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L142-50.
    Species: Mouse
    Sample Types: Cell Lysates
  88. Airway epithelial STAT3 is required for allergic inflammation in a murine model of asthma.
    Authors: Simeone-Penney MC, Severgnini M, Tu P, Homer RJ, Mariani TJ, Cohn L, Simon AR
    J. Immunol., 2007;178(10):6191-9.
    Species: Mouse
    Sample Types: BALF
  89. Modulation of airway remodeling and airway inflammation by peroxisome proliferator-activated receptor gamma in a murine model of toluene diisocyanate-induced asthma.
    Authors: Lee KS, Park SJ, Kim SR, Min KH, Jin SM, Lee HK, Lee YC
    J. Immunol., 2006;177(8):5248-57.
    Species: Mouse
    Sample Types: BALF
  90. Obligatory role for interleukin-13 in obstructive lesion development in airway allografts.
    Authors: Lama VN, Harada H, Badri LN, Flint A, Hogaboam CM, McKenzie A, Martinez FJ, Toews GB, Moore BB, Pinsky DJ
    Am. J. Pathol., 2006;169(1):47-60.
    Species: Mouse
    Sample Types: Tissue Homogenates
  91. Inhalation tolerance is induced selectively in thoracic lymph nodes but executed pervasively at distant mucosal and nonmucosal tissues.
    Authors: Alvarez D, Swirski FK, Yang TC, Fattouh R, Croitoru K, Bramson JL, Stampfli MR, Jordana M
    J. Immunol., 2006;176(4):2568-80.
    Species: Mouse
    Sample Types: Serum
  92. Experimental gastrointestinal allergy enhances pulmonary responses to specific and unrelated allergens.
    Authors: Brandt EB, Scribner TA, Akei HS, Rothenberg ME
    J. Allergy Clin. Immunol., 2006;118(2):420-7.
    Species: Mouse
    Sample Types: BALF
  93. Persistent effects induced by IL-13 in the lung.
    Authors: Fulkerson PC, Fischetti CA, Hassman LM, Nikolaidis NM, Rothenberg ME
    Am. J. Respir. Cell Mol. Biol., 2006;35(3):337-46.
    Species: Mouse
    Sample Types: BALF
  94. Allergic airway inflammation inhibits pulmonary antibacterial host defense.
    Authors: Beisswenger C, Kandler K, Hess C, Garn H, Felgentreff K, Wegmann M, Renz H, Vogelmeier C, Bals R
    J. Immunol., 2006;177(3):1833-7.
    Species: Mouse
    Sample Types: BALF
  95. Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
    Authors: Shibamori M, Ogino K, Kambayashi Y, Ishiyama H
    Life Sci., 2005;78(9):987-94.
    Species: Mouse
    Sample Types: BALF
  96. MyD88-dependent induction of allergic Th2 responses to intranasal antigen.
    Authors: Piggott DA, Eisenbarth SC, Xu L, Constant SL, Huleatt JW, Herrick CA, Bottomly K
    J. Clin. Invest., 2005;115(2):459-67.
    Species: Human
    Sample Types: Cell Culture Supernates
  97. Intranasal exposure to Stachybotrys chartarum enhances airway inflammation in allergic mice.
    Authors: Leino MS, Alenius HT, Fyhrquist-Vanni N, Wolff HJ, Reijula KE, Hintikka EL, Salkinoja-Salonen MS, Haahtela T, Mäkelä MJ
    Am. J. Respir. Crit. Care Med., 2005;173(5):512-8.
    Species: Mouse
    Sample Types: BALF
  98. Inhaled p38alpha mitogen-activated protein kinase antisense oligonucleotide attenuates asthma in mice.
    Authors: Duan W, Chan JH, McKay K, Crosby JR, Choo HH, Leung BP, Karras JG, Wong WS
    Am. J. Respir. Crit. Care Med., 2005;171(6):571-8.
    Species: Mouse
    Sample Types: BALF
  99. Vbeta14(+) T cells mediate the vaccine-enhanced disease induced by immunization with respiratory syncytial virus (RSV) G glycoprotein but not with formalin-inactivated RSV.
    Authors: Johnson TR, Varga SM, Braciale TJ, Graham BS
    J. Virol., 2004;78(16):8753-60.
    Species: Mouse
    Sample Types: Tissue Homogenates
  100. Effects of poly(ADP-ribose) polymerase inhibition on inflammatory cell migration in a murine model of asthma.
    Authors: Virag L, Bai P, Bak I, Pacher P, Mabley JG, Liaudet L, Bakondi E, Gergely P, Kollai M, Szabo C
    Med. Sci. Monit., 2004;10(3):BR77-83.
    Species: Mouse
    Sample Types: BALF
  101. Dual roles of IL-4 in lung injury and fibrosis.
    Authors: Huaux F, Liu T, McGarry B, Ullenbruch M, Phan SH
    J. Immunol., 2003;170(4):2083-92.
    Species: Mouse
    Sample Types: Serum
  102. Persistence of bronchopulmonary hyper-reactivity and eosinophilic lung inflammation after anti-IL-5 or -IL-13 treatment in allergic BALB/c and IL-4Ralpha knockout mice.
    Authors: Proust B, Nahori MA, Ruffie C, Lefort J, Vargaftig BB
    Clin. Exp. Allergy, 2003;33(1):119-31.
    Species: Mouse
    Sample Types: BALF
  103. Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice.
    Authors: Kobayashi T, Iijima K, Kita H
    J. Immunol., 2003;170(11):5756-63.
    Species: Mouse
    Sample Types: BALF
  104. Interleukin-9 induces mucous cell metaplasia independent of inflammation.
    Authors: Reader JR, Hyde DM, Schelegle ES, Aldrich MC, Stoddard AM, McLane MP, Levitt RC, Tepper JS
    Am. J. Respir. Cell Mol. Biol., 2003;28(6):664-72.
    Species: Mouse
    Sample Types: BALF
  105. IL-13 is necessary, not simply sufficient, for epicutaneously induced Th2 responses to soluble protein antigen.
    Authors: Herrick CA, Xu L, McKenzie AN, Tigelaar RE, Bottomly K
    J. Immunol., 2003;170(5):2488-95.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  106. MHC class II-associated invariant chain isoforms regulate pulmonary immune responses.
    Authors: Ye Q, Finn PW, Sweeney R, Bikoff EK, Riese RJ
    J. Immunol., 2003;170(3):1473-80.
    Species: Mouse
    Sample Types: BALF
  107. Absence of the complement anaphylatoxin C3a receptor suppresses Th2 effector functions in a murine model of pulmonary allergy.
    Authors: Drouin SM, Corry DB, Hollman TJ, Kildsgaard J, Wetsel RA
    J. Immunol., 2002;169(10):5926-33.
    Species: Mouse
    Sample Types: BALF
  108. IFN-gamma, but not Fas, mediates reduction of allergen-induced mucous cell metaplasia by inducing apoptosis.
    Authors: Shi ZO, Fischer MJ, De Sanctis GT, Schuyler MR, Tesfaigzi Y
    J. Immunol., 2002;168(9):4764-71.
    Species: Mouse
    Sample Types: BALF

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Mouse IL-13 Culture Media Quantikine ELISA
By Anonymous on 08/06/2018
Sample Tested: Cell culture supernatant

Mouse IL-13 Culture Media Quantikine ELISA
By Anonymous on 04/03/2018
Sample Tested: Serum

Mouse IL-13 Culture Media Quantikine ELISA
By Anonymous on 07/06/2017
Sample Tested: Mouse lung samples,Mouse lung lysates

This ELISA Kit from R&D System is amazing. Thanks