IL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.
Mouse IL-13 Culture Media Quantikine ELISA
R&D Systems | Catalog # M1300CB
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for Mouse IL-13 Culture Media Quantikine ELISA
Product Specifications
Measurement
Detection Method
Conjugate
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 33.9 | 100 | 432 | 31.6 | 93.4 | 405 |
| Standard Deviation | 1.1 | 2.7 | 11.2 | 2.5 | 7.2 | 17.7 |
| CV% | 3.2 | 2.7 | 2.6 | 7.9 | 7.7 | 4.4 |
Recovery for Mouse IL-13 Culture Media Quantikine ELISA
The recovery of mouse IL-13 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=13) | 99 | 89-119 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with mouse IL-13 were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse IL-13 Culture Media Quantikine ELISA
Mouse IL-13 ELISA Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: IL-13
Long Name
Alternate Names
Gene Symbol
Additional IL-13 Products
Product Documents for Mouse IL-13 Culture Media Quantikine ELISA
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IL-13 Culture Media Quantikine ELISA
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Mouse IL-13 Culture Media Quantikine ELISA
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Sample Tested: Cell culture supernatantVerified Customer | Posted 08/06/2018
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Sample Tested: SerumVerified Customer | Posted 04/03/2018
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Sample Tested: Mouse lung samples and Mouse lung lysatesVerified Customer | Posted 07/06/2017This ELISA Kit from R&D System is amazing. Thanks
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Protocols
View specific protocols for Mouse IL-13 Culture Media Quantikine ELISA (M1300CB):
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Mouse IL-13 Culture Media Quantikine ELISA
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Q: Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?
A: A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.
