Mouse IL-17/IL-17A Antibody [Biotin]

R&D Systems | Catalog # BAF421

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Western Blot, ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Detection), ELISpot Development, Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IL-17/IL-17A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL‑17 (R&D Systems, Catalog # 421-ML)
Thr22-Ala158
Accession # Q62386

Specificity

Detects mouse IL-17 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human IL‑17 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse IL-17/IL-17A Antibody [Biotin]

Mouse IL-17 / IL-17A ELISA Standard Curve

Mouse IL-17 / IL-17A ELISA Standard Curve

Recombinant Mouse IL‑17/IL‑17A (Catalog # 421-ML) was serially diluted and captured by Rat Anti-Mouse IL‑17/IL‑17A Monoclonal Antibody (Catalog # MAB721) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑17/IL‑17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-421-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse IL-17/IL-17A Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL‑17 (Catalog # 421-ML)

Mouse IL-17 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse IL‑17/IL‑17A Antibody (Catalog # MAB721)
  • Standard: Recombinant Mouse IL-17A Protein (Catalog # 421-ML)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 6 reviews rated 4.7 using BAF421 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17/IL-17A

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mouse IL-17 shows 57% and 87% sequence identity with herpes virus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

References

  1. Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611.
  2. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  3. Yao, Z. et al. (1995) Immunity 3:811.
  4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Gene Symbol

IL17A

UniProt

Q62386

Additional IL-17/IL-17A Products

Product Documents for Mouse IL-17/IL-17A Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-17/IL-17A Antibody [Biotin]

For research use only

Citations for Mouse IL-17/IL-17A Antibody [Biotin]

Customer Reviews for Mouse IL-17/IL-17A Antibody [Biotin] (6)

4.7 out of 5
6 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

Showing  1 - 5 of 6 reviews Showing All
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  • Mouse IL-17 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Mouse
    Verified Customer | Posted 11/27/2017
    This antibody was used as the detection in an ELISA with MAB421 as the capture.
    Mouse IL-17/IL-17A Antibody [Biotin] BAF421
  • Mouse IL-17 Biotinylated Antibody
    Name: Boubacar Barry
    Application: Flow Cytometry
    Sample Tested: 293T human embryonic kidney cell line
    Species: oct
    Verified Customer | Posted 08/11/2017
  • Mouse IL‑17 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Bone marrow-derived dendritic cells, Mouse spleenocytes and Liver tissue
    Species: Mouse
    Verified Customer | Posted 02/22/2016
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID: 22491249
    Species: Mouse
    Verified Customer | Posted 01/14/2015
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID: 21469121
    Species: Mouse
    Verified Customer | Posted 01/14/2015
  • Name: Anonymous
    Application: ELISA
    Sample Tested: See PMID: 21398615
    Species: Mouse
    Verified Customer | Posted 01/14/2015

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