Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mouse IL-17 shows 57% and 87% sequence identity with herpes virus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.
Mouse IL‑17 Biotinylated Antibody
R&D Systems | Catalog # BAF421
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat, Transgenic Mouse
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
Western Blot, ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Detection), ELISpot Development, Luminex Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse IL‑17 (R&D Systems, Catalog # 421-ML)
Thr22-Ala158
Accession # Q62386
Thr22-Ala158
Accession # Q62386
Specificity
Detects mouse IL-17 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human IL‑17 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse IL‑17 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IL‑17 (Catalog # 421-ML)
Sample: Recombinant Mouse IL‑17 (Catalog # 421-ML)
Mouse IL-17 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 6 reviews rated 4.7 using BAF421 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-17/IL-17A
References
- Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611.
- Yao, Z. et al. (1995) J. Immunol. 155:5483.
- Yao, Z. et al. (1995) Immunity 3:811.
- Rouvier, E. et al. (1993) J. Immunol. 150:5445.
Long Name
Interleukin 17
Alternate Names
CTLA-8, CTLA8, IL-17A, IL17, IL17A
Entrez Gene IDs
Gene Symbol
IL17A
UniProt
Additional IL-17/IL-17A Products
Product Documents for Mouse IL‑17 Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IL‑17 Biotinylated Antibody
For research use only
Citations for Mouse IL‑17 Biotinylated Antibody
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Customer Reviews for Mouse IL‑17 Biotinylated Antibody (6)
4.7 out of 5
6 Customer Ratings
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Application: ELISASample Tested: Serum and PlasmaSpecies: MouseVerified Customer | Posted 11/27/2017This antibody was used as the detection in an ELISA with MAB421 as the capture.
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Application: Flow CytometrySample Tested: 293T human embryonic kidney cell lineSpecies: octVerified Customer | Posted 08/11/2017
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Application: ELISASample Tested: Bone marrow-derived dendritic cells, Mouse spleenocytes and Liver tissueSpecies: MouseVerified Customer | Posted 02/22/2016
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Application: ELISASample Tested: See PMID: 22491249Species: MouseVerified Customer | Posted 01/14/2015
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Application: ELISASample Tested: See PMID: 21469121Species: MouseVerified Customer | Posted 01/14/2015
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Application: ELISASample Tested: See PMID: 21398615Species: MouseVerified Customer | Posted 01/14/2015
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
