Mouse IL-17E/IL-25 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse IL‑17E/IL-25 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human IL‑17E, recombinant mouse IL‑17, IL‑17B, C, D, or F.
  • Source
    Monoclonal Rat IgG2B Clone # 207702
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse IL‑17E/IL-25
    Accession # NP_542767
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑17E/IL‑25 in Tramp‑C1 Mouse Cell Line by Flow Cytometry. Tramp‑C1 mouse prostate cancer cell line was stained with Rat Anti-Mouse IL‑17E/IL‑25 PE‑conjugated Monoclonal Antibody (Catalog # IC13991P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-17E/IL-25

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Mouse IL-17E cDNA encodes a 169 amino acid residues (aa) precursor protein with a putative 16 aa signal peptide (5). Mature mouse IL-17E shares 76% and 91% amino sequence (aa) identity with mature human and rat IL-17E, respectively. Mouse IL-17E also shares from 24-32% sequence identity with the other mouse IL-17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord, and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the pro-inflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).

  • References:
    1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
    2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
    3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
    4. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
    5. Pan, G. et al. (2001) J. Immunol. 167:6569.
  • Long Name:
    Interleukin 17E
  • Entrez Gene IDs:
    64806 (Human); 140806 (Mouse); 501996 (Rat)
  • Alternate Names:
    IL17E; IL-17E; IL17Einterleukin-17E; IL25; IL-25; interleukin 17E; interleukin 25; Interleukin-17E; interleukin-25
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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