Mouse IL-17E/IL-25 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC13991P
Mouse IL-17E/IL-25 PE-conjugated Antibody in Data
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Mouse IL-17E/IL-25 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑17E/IL-25 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human IL‑17E, recombinant mouse IL‑17, IL‑17B, C, D, or F.
Source
Monoclonal Rat IgG2B Clone # 207702
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL‑17E/IL-25
Val17-Ala169
Accession # NP_542767
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of IL‑17E/IL‑25 in Tramp‑C1 Mouse Cell Line by Flow Cytometry. View Larger

Detection of IL‑17E/IL‑25 in Tramp‑C1 Mouse Cell Line by Flow Cytometry. Tramp-C1 mouse prostate cancer cell line was stained with Rat Anti-Mouse IL-17E/IL-25 PE-conjugated Monoclonal Antibody (Catalog # IC13991P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17E/IL-25

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Mouse IL-17E cDNA encodes a 169 amino acid residues (aa) precursor protein with a putative 16 aa signal peptide (5). Mature mouse IL-17E shares 76% and 91% amino sequence (aa) identity with mature human and rat IL-17E, respectively. Mouse IL-17E also shares from 24-32% sequence identity with the other mouse IL-17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord, and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the pro-inflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
  4. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
  5. Pan, G. et al. (2001) J. Immunol. 167:6569.
Long Name
Interleukin 17E
Entrez Gene IDs
64806 (Human); 140806 (Mouse); 501996 (Rat)
Alternate Names
IL17E; IL-17E; IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25

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