Detects mouse IL-1 beta /IL-1F2 in direct ELISAs. In direct ELISAs, approximately 75% cross-reactivity with recombinant guinea pig IL-1 beta, 5‑15% cross-reactivitiy with recombinant canine, cotton rat, feline, equine, and rat IL-1 beta, and no cross-reactivity with recombinant human, porcine, and rhesus IL-1 beta is observed. No cross-reactivity with recombinant human (rh) Pro-IL-1 beta, rhIL-1F7, rhIL-1F10, rhIL-36 gamma, recombinant mouse (rm) IL-1 alpha, rmIL-18, rmIL-36Ra, rmIL-36 alpha, or rmIL-36 beta is observed.
Monoclonal Rat IgG2B Clone # 166931
Protein A or G purified from hybridoma culture supernatant
Detection of IL‑1 beta /IL‑1F2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes, untreated (open histogram), or treated with LPS for 48 hours (filled histogram) were stained with Rat Anti-Mouse IL‑1 beta /IL‑1F2 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC4013F, filled histogram) or isotype control antibody (Catalog # IC013F, data not shown). Histograms were gated on blasting splenocytes. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-1 beta/IL-1F2
IL-1 beta is a pro-inflammatory cytokine belonging to the IL-1 superfamily, designated IL-1F2. IL-1 beta and IL-1 alpha /IL-1F1 bind the same receptors but vary in expression and affinity. They share only 25% amino acid (aa) sequence identity and are produced from separate genes. IL-1 beta is synthesized as a 31 kDa pro-form that is cleaved intracellularly by caspase-1 to produce the mature active 17.5 kDa IL-1 beta. Mature mouse IL-1 beta shares 74% and 91% aa identity with human and rat IL-1 beta, respectively.
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