Mouse IL-20 Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-20 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2B Clone # 380605
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse IL‑20
    Leu25-Leu176
    Accession # Q9JKV9.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize IL‑20-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑20.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑20 and Neutralization by Mouse IL‑20 Antibody. Recombinant Mouse IL‑20 (Catalog # 1204-ML) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑20 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑20 Monoclonal Antibody (Catalog # MAB12043). The ND50 is typically 2-8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-20

Mouse Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences with a signal sequence and amphipathic helices found in helical cytokines. Based on the human molecule, mouse IL-20 was discovered in a skin library. Mouse IL-20 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL-20 are 77% aa identical in the mature segment. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first complex is composed of IL-20 R alpha and IL‑20 R beta. The second complex is composed of IL-22 R and IL-20 R beta. Whereas the IL-22 R/IL‑20 R beta complex is shared with IL-24/mda-7, the IL‑20 R alpha /IL‑20 R beta complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of pro-inflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1-6).

  • References:
    1. Blumberg, H. et al. (2001) Cell 104:9.
    2. Liu, L. et al. (2003) Blood 102:3206.
    3. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.
    4. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
    5. Dumoutier, L. (2001) J. Immunol. 167:3545. 
    6. Romer, J. (2003) J. Invest. Dermatol. 121:1306.
  • Long Name:
    Interleukin 20
  • Entrez Gene IDs:
    50604 (Human); 58181 (Mouse)
  • Alternate Names:
    Cytokine Zcyto10; IL10D; IL20; IL-20; IL-20ZCYTO10four alpha helix cytokine; interleukin 20; interleukin-20; MGC96907
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines.
    Authors: Uto-Konomi A, Miyauchi K, Ozaki N
    PLoS ONE, 2012;7(7):e40343.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Isotype Controls
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Rat IgG2B Isotype Control

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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2B Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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