Mouse IL-22BP APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC2376A
Detection of IL‑22BP in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Mouse IL-22BP APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-22BP in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) IL-22BP is observed and 5% cross-reactivity with rhIL-22 R is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑22BP
Thr19-Pro230
Accession # AAI24999
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL‑22BP antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑22BP in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse IL-22BP APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2376A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-22BP

Interleukin 22 Binding Protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35‑45 kDa secreted glycoprotein belonging to the Type II Cytokine Receptor Family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is also a component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29 (1-4). IL-10 R beta is widely expressed, and other cell types express either IL-10 R alpha or IL-22 R but not both (5). The mouse IL-22BP cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence and two fibronectin type-III domains (6). Mouse IL-22BP shares 59% and 85% aa sequence identity with human and rat IL-22BP, respectively. Among type II cytokine receptors, IL-22BP is most closely related to the extracellular domain of IL-22 R, sharing 36% aa sequence identity. Splice variants of human IL-22BP exist but have not been detected for the mouse protein (6-8). IL-22BP binds an epitope on IL-22 that is required for the interaction of IL-22 with the transmembrane IL-22 R (8-10), thereby preventing the association of IL-22 R with IL-10 R beta (10). IL-22BP blocks IL-22-induced responses including induction of MHC class I expression, Stat1 and Stat3 activation, SOCS3 gene expression,the production of reactive oxygen species (6, 9) and the modulation of the proinflammatory effects of IL-22 (11). Mouse IL-22 BP neutralizes both mouse and human IL-22 (6). IL-22BP is secreted by epithelial cells, dendritic cells, activated B cells, and activated monocytes (6, 8, 12, 13).

References
  1. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
  2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  3. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  4. Kotenko, S.V. (2002) Cytokine Growth Factor Rev. 13:223.
  5. Wolk, K. et al. (2005) Genes Immun. 6:8.
  6. Wei, C.C. et al. (2003) Genes Immun. 4:204.
  7. Dumoutier, L. et al. (2001) J. Immunol. 166:7090.
  8. Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.
  9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  10. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  11. Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220.
  12. Lecart, S. et al. (2002) Int. Immunol. 14:1351.
  13. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
Long Name
Interleukin 22 Binding Protein
Entrez Gene IDs
116379 (Human); 237310 (Mouse)
Alternate Names
class II cytokine receptor; CRF2-10; CRF2-S1IL22BP; CRF2-X; Cytokine receptor class-II member 10; Cytokine receptor family 2 member 10; Cytokine receptor family type 2, soluble 1; IL-22 RA2; IL-22 receptor subunit alpha-2; IL22BP; IL-22BP; IL-22BPCRF2X; IL22RA2; IL-22RA2; IL-22R-alpha-2; interleukin 22 receptor, alpha 2; interleukin 22-binding protein; interleukin-22 receptor subunit alpha-2; Interleukin-22-binding protein; MGC150509; MGC150510; zcytoR16

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