Detection of IL‑23 R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 50 ng/mL PMA, 200 ng/mL Calcium Ionomycin, 20 ng/mL Recombinant Mouse IL‑23 (Catalog # 1887-ML), 40 ng/mL Recombinant Mouse IL‑6 (Catalog # 406-ML), and 200 ng/mL LPS overnight to induce Th17 activation were stained with Rat Anti-Mouse CD3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB4841G) and either (A) Rat Anti-Mouse IL‑23 R PerCP‑conjugated Monoclonal Antibody (Catalog # FAB16861C) or (B) Rat IgG1 PerCP Isotype Control (Catalog # IC005C). View our protocol for Staining Membrane-associated Proteins.
Detection of IL‑23 R in HEK293 Human Cell Line Transfected with Mouse IL-23 R by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse IL-23 R or (B) irrelevant transfectants was stained with Rat Anti-Mouse IL‑23 R PerCP‑conjugated Monoclonal Antibody (Catalog # FAB16861C, filled histogram) or isotype control antibody (Catalog # IC005C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-23 R
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4+ CD45RBlow memory T cells but at much lower levels by mouse CD4+ CD45RBhigh cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.
Oppmann, B. et al. (2000) Immunity 13:715.
Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
Parham, C. et al. (2002) J. Immunol. 168:5448.
Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
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