Detection of CD3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CD3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB4841G) or (B) Rat IgG2B Alexa Fluor 488 Isotype Control (Catalog # IC013G). View our protocol for Staining Membrane-associated Proteins.
CD3 in Mouse Splenocytes. CD3 was detected and stained in immersion fixed adult mouse splenocytes using Rat Anti-Mouse CD3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (green; Catalog # FAB4841G) at 5 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma (1). This antibody has been shown to react with the epsilon chain of the CD3 complex (1). The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.
Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
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