Mouse IL-27 p28/IL-30 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
M2728
Mouse IL-27 p28/IL-30 ELISA Standard Curve
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Product Details
Procedure
Citations (13)
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Reviews (3)

Mouse IL-27 p28/IL-30 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
4.7 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse IL-27 p28
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse IL-27 p28 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-27 p28 in cell culture supernates, mouse serum, and plasma. It contains NS0-expressed recombinant mouse IL-27 p28 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-27 p28 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IL-27 p28.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 31 33 37
Mean 20 49.2 228 24.1 56.7 238
Standard Deviation 1.8 3.4 14.6 2.3 4.5 21.3
CV% 9 6.9 6.4 9.5 7.9 8.9

Recovery

The recovery of mouse IL-27 p28 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=8) 102 83-112
EDTA Plasma (n=8) 99 83-116
Heparin Plasma (n=8) 95 87-101
Serum (n=8) 95 85-102

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse IL-27 p28 in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse IL-27 p28/IL-30 ELISA Linearity

Data Examples

Mouse IL-27 p28/IL-30 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-27 p28/IL-30

IL-27 is a heterodimeric cytokine that is composed of EBI3 (which is related to the p40 subunit of IL-12 and IL-23) and p28 (which is related to the p35 chain of IL-12). IL-27 is expressed by monocytes, endothelial cells, and dendritic cells, and it signals through a receptor complex composed of IL-27 R alpha/WSX-1/TCCR and gp130. IL-27 induces IL-12 receptor expression on naïve CD4+ T cells, sensitizing them to subsequent IL-12 induced Th1 development. It also functions as an anti-inflammatory cytokine by limiting T and NKT cell activity.

Long Name:
Interleukin 27 p28
Entrez Gene IDs:
246778 (Human); 246779 (Mouse)
Alternate Names:
IL27 p28; IL-27 p28; IL-30
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse IL-27 p28/IL-30 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway.
    Authors: Sha X, Meng S, Li X, Xi H, Maddaloni M, Pascual D, Shan H, Jiang X, Wang H, Yang X
    J Biol Chem, 2015;290(31):19307-18.
    Species: Mouse
    Sample Types: Plasma
  2. IL-27R-mediated regulation of IL-17 controls the development of respiratory syncytial virus-associated pathogenesis.
    Authors: de Almeida Nagata D, Demoor T, Ptaschinski C, Ting H, Jang S, Reed M, Mukherjee S, Lukacs N
    Am J Pathol, 2014;184(6):1807-18.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. IL-10-Producing Th1 Cells and Disease Progression Are Regulated by Distinct CD11c+ Cell Populations during Visceral Leishmaniasis.
    Authors: Owens BM, Beattie L, Moore JW, Brown N, Mann JL, Dalton JE, Maroof A, Kaye PM
    PLoS Pathog., 2012;8(7):e1002827.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Critical role of the IFN-stimulated gene factor 3 complex in TLR-mediated IL-27p28 gene expression revealing a two-step activation process.
    Authors: Molle C, Goldman M, Goriely S
    J. Immunol., 2010;184(4):1784-92.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. A role for IL-27p28 as an antagonist of gp130-mediated signaling.
    Authors: Stumhofer JS, Tait ED, Quinn WJ, Hosken N, Spudy B, Goenka R, Fielding CA, O'Hara AC, Chen Y, Jones ML, Saris CJ, Rose-John S, Cua DJ, Jones SA, Elloso MM, Grotzinger J, Cancro MP, Levin SD, Hunter CA
    Nat. Immunol., 2010;11(12):1119-26.
    Species: Mouse
    Sample Types: Serum
  6. Modified adenine (9-benzyl-2-butoxy-8-hydroxyadenine) redirects Th2-mediated murine lung inflammation by triggering TLR7.
    Authors: Vultaggio A, Nencini F, Fitch PM, Fili L, Maggi L, Fanti P, deVries A, Beccastrini E, Palandri F, Manuelli C, Bani D, Giudizi MG, Guarna A, Annunziato F, Romagnani S, Maggi E, Howie SE, Parronchi P
    J. Immunol., 2009;182(2):880-9.
    Species: Mouse
    Sample Types: Serum
  7. The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice.
    Authors: Guo B, Chang EY, Cheng G
    J. Clin. Invest., 2008;118(5):1680-90.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: role of intracellular osteopontin.
    Authors: Shinohara ML, Kim JH, Garcia VA, Cantor H
    Immunity, 2008;29(1):68-78.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3.
    Authors: Molle C, Nguyen M, Flamand V, Renneson J, Trottein F, De Wit D, Willems F, Goldman M, Goriely S
    J. Immunol., 2007;178(12):7607-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. The proinflammatory effect of prostaglandin E2 in experimental inflammatory bowel disease is mediated through the IL-23-->IL-17 axis.
    Authors: Sheibanie AF, Yen JH, Khayrullina T, Emig F, Zhang M, Tuma R, Ganea D
    J. Immunol., 2007;178(12):8138-47.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Regulation of IL-27 p28 gene expression in macrophages through MyD88- and interferon-gamma-mediated pathways.
    Authors: Liu J, Guan X, Ma X
    J. Exp. Med., 2007;204(1):141-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Suppressive effect of IL-27 on encephalitogenic Th17 cells and the effector phase of experimental autoimmune encephalomyelitis.
    Authors: Fitzgerald DC, Ciric B, Touil T, Harle H, Grammatikopolou J, Das Sarma J, Gran B, Zhang GX, Rostami A
    J. Immunol., 2007;179(5):3268-75.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Peroxisome proliferator-activated receptor-alpha agonist fenofibrate regulates IL-12 family cytokine expression in the CNS: relevance to multiple sclerosis.
    Authors: Xu J, Racke MK, Drew PD
    J. Neurochem., 2007;103(5):1801-10.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Mouse IL-27 p28/IL-30 Quantikine ELISA Kit
By Anonymous on 05/06/2019
Application: Sample Tested: Cell culture supernatant

Mouse IL-27 p28/IL-30 Quantikine ELISA Kit
By Anonymous on 08/06/2018
Application: Sample Tested: Cell Culture Supernates

Mouse IL-27 p28/IL-30 Quantikine ELISA Kit
By Anonymous on 06/28/2017
Application: Sample Tested: Serum