Mouse IL-33 PE-conjugated Antibody

(7 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-33 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 396118
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse IL-33
    Ser109-Ile266
    Accession # Q8BVZ5
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑33 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti‑Mouse IL‑33 PE‑conjugated Mono­clonal Antibody (Catalog # IC3626P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% amino acid (aa) sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

  • References:
    1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
    2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
    3. Schmitz, J. et al. (2005) Immunity 23:479.
    4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
    5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
    6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
    7. Dinarello, C.A. (2005) Immunity 23:461.
    8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
    9.  
  • Long Name:
    Interleukin 33
  • Entrez Gene IDs:
    90865 (Human); 77125 (Mouse)
  • Alternate Names:
    C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. IL-33/ST2-mediated inflammation in macrophages is directly abrogated by IL-10 during rheumatoid arthritis
    Authors: S Chen, B Chen, Z Wen, Z Huang, L Ye
    Oncotarget, 2017;8(20):32407-32418.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Type II cytokines impair host defense against an intracellular fungal pathogen by amplifying macrophage generation of IL-33.
    Authors: Verma, A, Kroetz, D N, Tweedle, J L, Deepe, G S Jr
    Mucosal Immunol, 2015;8(2):380-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins alpha-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators.
    Authors: Kempuraj D, Thangavel R, Yang E, Pattani S, Zaheer S, Santillan D, Santillan M, Zaheer A
    PLoS ONE, 2015;10(8):e0135776.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Murine mast cells secrete and respond to interleukin-33.
    Authors: Tung, Hui-Ying, Plunkett, Beverly, Huang, Shau-Ku, Zhou, Yufeng
    J Interferon Cytokine Res, 2014;34(3):141-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
    Authors: Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F
    Mucosal Immunol, 2014;7(5):1079-93.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
    Authors: Mizutani N, Nabe T, Yoshino S
    Immunology, 2013;139(2):205-18.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Trefoil factor 2 rapidly induces interleukin 33 to promote type 2 immunity during allergic asthma and hookworm infection.
    Authors: Wills-Karp M, Rani R, Dienger K
    J. Exp. Med., 2012;209(3):607-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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Average Rating: 2 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Mouse.
We have 2 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Mouse IL-33 PE-conjugated Antibody IC3626P
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 Flow Mouse Anonymous 08/30/2016
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Flow Cytometry Mouse IL-33 PE-conjugated Antibody IC3626P
Flow Cytometry: Mouse IL-33 PE-conjugated Antibody [IC3626P]

Summary

ApplicationFlow Cytometry
Sample TestedLung single-cell suspension
SpeciesMouse

Other Experimental Details

Other Experimental DetailsCells were activated with PMA and ionomycin for 2 hrs before intracellular staining.
Flow Cytometry Mouse IL-33 PE-conjugated Antibody IC3626P
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 Flow Mouse Anonymous 07/25/2016
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Image Details
Flow Cytometry Mouse IL-33 PE-conjugated Antibody IC3626P
Flow Cytometry: Mouse IL-33 PE-conjugated Antibody [IC3626P]

Summary

ApplicationFlow Cytometry
Sample Testedmast cells
SpeciesMouse

Other Experimental Details

Other Experimental DetailsMast cells were cultured from the bone marrow of IL-33-deficient or WT C57BL/6 mice. Genotypes were confirmed by PCR. Cells (5x10^5) were stained for viability, FceRI and c-kit, blocking Fc receptors, then fixed with BD Cytofix/Cytoperm. Cells were permeabilized with BD Permeabilization Buffer, and stained with 2ul anti-mouse IL-33-PE or rat IgG2a-PE isotype control. Cells were washed three times, and data were acquired on a BD FACSCanto LSR flow cytometer, gating on live c-Kit+FceRI+ cells.Fluorescence increases by two orders of magnitude in both WT and IL-33-KO cells following staining with the IL-33-PE antibody relative to the isotype control. I am currently investigating whether there is any specific signal after stimulation of the cells, at which point IL-33 is induced. Staining with the recommended (greater) amount of antibody produces an even greater background shift in unstimulated cells.R&D Systems Technical Service is investigating.

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