Mouse IL-34 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-34 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-34 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 780310
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL-34
    Asn21-Pro235
    Accession # Q8R1R4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.01 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize IL‑34-induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. The Neutralization Dose (ND50) is typically 0.1-0.5 ug/mL in the presence of 100 ng/mL Recombinant Mouse IL‑34.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑34 and Neutralization by Mouse IL‑34 Antibody. Recombinant Mouse IL‑34 induces proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in the presence of in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL‑34 is neutralized (green line) by increasing concen­trations of Rat Anti-Mouse IL‑34 Monoclonal Antibody (Catalog # MAB5195). The ND50 is typically 0.1-0.5 ug/mL.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77 homolog) is a 39 kDa, secreted cytokine that belongs to no known cytokine family (1). Mouse IL-34 is synthesized as a 235 amino acid (aa) precursor with a 20 aa signal sequence and a 215 aa mature chain that contains two potential sites for N-linked glycosylation. There are three isoforms for IL-34. Isoform 1 (Q8R1R4-1) is the recombinant mouse IL-34 described in this insert. Isoform 2 (Q8R1R4-2) has an 85 aa substitution for the final 101 aa in isoform 1, and isoform 3 (Q8R1R4-3) lacks Q81 in isoform 1. Mouse IL-34 shares 71% aa sequence identity with human IL‑34. IL‑34 is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and it is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF‑1R) (1). IL-34 stimulates monocyte viability (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

  • References:
    1. Lin, H. et al. (2008) Science 320:807.
  • Long Name:
    Interleukin 34
  • Entrez Gene IDs:
    146433 (Human); 76527 (Mouse)
  • Alternate Names:
    C16orf77; IL34; IL-34
Related Research Areas
Isotype Controls
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Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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