Mouse IL-34 Antibody

Catalog # Availability Size / Price Qty
MAB5195
MAB5195-SP

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Cell Proliferation Induced by IL‑34 and Neutralization by Mouse IL‑34 Antibody.
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Mouse IL-34 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-34 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-34 is observed.
Source
Monoclonal Rat IgG2A Clone # 780310
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-34
Asn21-Pro235
Accession # Q8R1R4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IL‑34-induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. The Neutralization Dose (ND50) is typically 0.1-0.5 ug/mL in the presence of 100 ng/mL Recombinant Mouse IL‑34.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑34 and Neutralization by Mouse IL‑34 Antibody. View Larger

Cell Proliferation Induced by IL‑34 and Neutralization by Mouse IL‑34 Antibody. Recombinant Mouse IL-34 induces proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in the presence of in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-34 is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse IL-34 Monoclonal Antibody (Catalog # MAB5195). The ND50 is typically 0.1-0.5 ug/mL.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77 homolog) is a 39 kDa, secreted cytokine that belongs to no known cytokine family (1). Mouse IL-34 is synthesized as a 235 amino acid (aa) precursor with a 20 aa signal sequence and a 215 aa mature chain that contains two potential sites for N-linked glycosylation. There are three isoforms for IL-34. Isoform 1 (Q8R1R4-1) is the recombinant mouse IL-34 described in this insert. Isoform 2 (Q8R1R4-2) has an 85 aa substitution for the final 101 aa in isoform 1, and isoform 3 (Q8R1R4-3) lacks Q81 in isoform 1. Mouse IL-34 shares 71% aa sequence identity with human IL‑34. IL‑34 is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and it is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF‑1R) (1). IL-34 stimulates monocyte viability (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

References
  1. Lin, H. et al. (2008) Science 320:807.
Long Name
Interleukin 34
Entrez Gene IDs
146433 (Human); 76527 (Mouse)
Alternate Names
C16orf77; IL34; IL-34; interleukin 34

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Citation for Mouse IL-34 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inhibition of IL-34 Unveils Tissue-Selectivity and Is Sufficient to Reduce Microglial Proliferation in a Model of Chronic Neurodegeneration.
    Authors: Obst J, Simon E, Martin-Estebane M, Pipi E, Barkwill L, Gonzalez-Rivera I, Buchanan F, Prescott A, Faust D, Fox S, Brownlees J, Taylor D, Perry V, Nuthall H, Atkinson P, Karran E, Routledge C, Gomez-Nicola D
    Front Immunol, 2020-10-08;11(0):579000.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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