IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL)
Sensitivity
7 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum)
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Product Summary for Mouse IL-5 Quantikine ELISA Kit
The Quantikine Mouse IL-5 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-5 in cell culture supernates and mouse serum. It contains Sf 21-expressed recombinant mouse IL-5 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant mouse IL-5. Results obtained using natural mouse IL-5 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-5.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse IL-5
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 41.5 | 117 | 428 | 43.4 | 123 | 447 |
| Standard Deviation | 2.4 | 3.9 | 8.6 | 1.8 | 5.3 | 20.8 |
| CV% | 5.8 | 3.3 | 2.0 | 4.1 | 4.3 | 4.7 |
Recovery for Mouse IL-5 Quantikine ELISA Kit
The recovery of mouse IL-5 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 99 | 85-110 |
| Serum (n=5) | 92 | 81-104 |
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-5 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse IL-5 Quantikine ELISA Kit
Mouse IL-5 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-5
Long Name
Interleukin 5
Alternate Names
BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF
Entrez Gene IDs
Gene Symbol
IL5
Additional IL-5 Products
Product Documents for Mouse IL-5 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IL-5 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Mouse IL-5 Quantikine ELISA Kit
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Customer Reviews for Mouse IL-5 Quantikine ELISA Kit (3)
4.3 out of 5
3 Customer Ratings
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Sample Tested: Mouse peritoneal washVerified Customer | Posted 12/31/2020The C57BL mice was I.P. challenged by gram-negative bacteria Acinetobacter baumannii and the peritoneal wash was collected at 5 hours post infection. The IL-5 was measured by ELISA.
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Sample Tested: SerumVerified Customer | Posted 08/06/2018
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Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line and Lung tissueVerified Customer | Posted 05/02/2017
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Protocols
View specific protocols for Mouse IL-5 Quantikine ELISA Kit (M5000):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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