Mouse IL-5 Quantikine ELISA Kit

  (66 citations)
(1 Review)
    
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Product Details
Assay Procedure
Citations (66)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL)
  • Sensitivity
    7 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)
  • Specificity
    Natural and recombinant mouse IL-5
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IL-5 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-5 in cell culture supernates and mouse serum. It contains Sf 21-expressed recombinant mouse IL-5 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant mouse IL-5. Results obtained using natural mouse IL-5 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-5.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 41.5 117 428 43.4 123 447
Standard Deviation 2.4 3.9 8.6 1.8 5.3 20.8
CV% 5.8 3.3 2 4.1 4.3 4.7

Recovery

The recovery of mouse IL-5 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 99 85-110
Serum (n=5) 92 81-104
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-5 in each matrix were diluted with Calibrator Diluent and then assayed.
 IL-5 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-5
IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.
  • Long Name:
    Interleukin 5
  • Entrez Gene IDs:
    3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
  • Alternate Names:
    BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

66 Citations: Showing 1 - 10
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Species
Sample Type
  1. Eosinophil-derived CCL-6 impairs hematopoietic stem cell homeostasis
    Authors: C Zhang, W Yi, F Li, X Du, H Wang, P Wu, C Peng, M Luo, W Hua, CC Wong, JJ Lee, W Li, Z Chen, S Ying, Z Ju, H Shen
    Cell Res., 2018;0(0):.
    Species: Mouse
    Sample Type: Serum
  2. Justicia procumbens Extract (DW2008) Selectively Suppresses Th2 Cytokines in Splenocytes and Ameliorates Ovalbumin-Induced Airway Inflammation in a Mouse Model of Asthma
    Authors: J Youm, H Lee, HB Chang, J Jeon, MH Yoon, JY Woo, MS Choi, Y Hwang, S Seong, K Na, J Yoon
    Biol. Pharm. Bull., 2017;40(9):1416-1422.
    Species: Mouse
    Sample Type: BALF
  3. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
    Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
  4. Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine
    Authors: YT Lee, CT Wu, HL Sun, JL Ko, KH Lue
    J Microbiol Immunol Infect, 2017;0(0):.
    Species: Mouse
    Sample Type: BALF
  5. Bach2 Controls Homeostasis of Eosinophils by Restricting the Type-2 Helper Function of T Cells
    Authors: Y Sato, H Kato, R Ebina-Shib, A Itoh-Nakad, R Okuyama, K Igarashi
    Tohoku J. Exp. Med, 2017;241(3):175-182.
    Species: Mouse
    Sample Type: Serum
  6. Beneficial effects of Galectin-9 on allergen-specific sublingual immunotherapy in a Dermatophagoides farinae-induced mouse model�of chronic asthma
    Allergol Int, 2016;0(0):.
    Species: Mouse
    Sample Type: Complex Sample Type
  7. G-protein-coupled estrogen receptor agonist suppresses airway inflammation in a mouse model of asthma through IL-10.
    Authors: Itoga M, Konno Y, Moritoki Y, Saito Y, Ito W, Tamaki M, Kobayashi Y, Kayaba H, Kikuchi Y, Chihara J, Takeda M, Ueki S, Hirokawa M
    PLoS ONE, 2015;10(3):e0123210.
    Species: Mouse
    Sample Type: BALF
  8. IL-33-mediated protection against experimental cerebral malaria is linked to induction of type 2 innate lymphoid cells, M2 macrophages and regulatory T cells.
    Authors: Besnard A, Guabiraba R, Niedbala W, Palomo J, Reverchon F, Shaw T, Couper K, Ryffel B, Liew F
    PLoS Pathog, 2015;11(2):e1004607.
    Species: Mouse
    Sample Type: Serum
  9. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
    Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
    Nat Commun, 2015;6(0):6224.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  10. Cyclooxygenase inhibition abrogates aeroallergen-induced immune tolerance by suppressing prostaglandin I2 receptor signaling.
    Authors: Zhou W, Goleniewska K, Zhang J, Dulek D, Toki S, Lotz M, Newcomb D, Boswell M, Polosukhin V, Milne G, Wu P, Moore M, FitzGerald G, Peebles R
    J Allergy Clin Immunol, 2014;134(3):698-705.e5.
    Species: Mouse
    Sample Type: BALF
  11. Eosinophils regulate peripheral B cell numbers in both mice and humans.
    Authors: Wong, Tina W, Doyle, Alfred D, Lee, James J, Jelinek, Diane F
    J Immunol, 2014;192(8):3548-58.
    Species: Mouse
    Sample Type: Serum
  12. Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice.
    Authors: Mizutani N, Nabe T, Yoshino S
    Immunology, 2013;139(2):205-18.
    Species: Mouse
    Sample Type: Tissue Homogenates
  13. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
    Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
    Am. J. Pathol., 2012;179(1):302-14.
    Species: Mouse
    Sample Type: Tissue Homogenates
  14. Control of mouse graft-versus-host disease following allogeneic bone marrow transplantation by blocking the CD28/B7 signaling pathway with lentiviral vector-mediated RNA interference.
    Authors: Sang W, Zhou C, Cheng N, Li Z, Zeng L, Xu K
    Immunol. Lett., 2011;136(2):194-202.
    Species: Mouse
    Sample Type: Plasma
  15. Endogenous IL-33 enhances Th2 cytokine production and T-cell responses during allergic airway inflammation.
    Authors: Louten J, Rankin AL, Li Y
    Int. Immunol., 2011;23(5):307-15.
    Species: Mouse
    Sample Type: BALF
  16. IL-33-activated dendritic cells are critical for allergic airway inflammation.
    Authors: Besnard AG, Togbe D, Guillou N, Erard F, Quesniaux V, Ryffel B
    Eur. J. Immunol., 2011;41(6):1675-86.
    Species: Mouse
    Sample Type: BALF
  17. Over-expression of the LTC4 synthase gene in mice reproduces human aspirin-induced asthma.
    Authors: Hirata H, Arima M, Fukushima Y, Honda K, Sugiyama K, Tokuhisa T, Fukuda T
    Clin. Exp. Allergy, 2011;41(8):1133-42.
    Species: Mouse
    Sample Type: BALF
  18. IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs.
    Authors: Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H
    J. Immunol., 2011;188(3):1503-13.
    Species: Mouse
    Sample Type: BALF
  19. Epithelial reticulon 4B (Nogo-B) is an endogenous regulator of Th2-driven lung inflammation.
    Authors: Wright PL, Yu J, Di YP
    J. Exp. Med., 2010;207(12):2595-607.
    Species: Mouse
    Sample Type: BALF
  20. Complement C3a regulates late asthmatic response and airway hyperresponsiveness in mice.
    Authors: Mizutani N, Nabe T, Yoshino S
    J. Immunol., 2009;183(6):4039-46.
    Species: Mouse
    Sample Type: BALF
  21. Mouse models of asthma: a comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness, inflammation, and cytokine production.
    Authors: Gueders MM, Paulissen G, Crahay C, Quesada-Calvo F, Hacha J, Van Hove C, Tournoy K, Louis R, Foidart JM, Noel A, Cataldo DD
    Inflamm. Res., 2009;58(12):845-54.
    Species: Mouse
    Sample Type: BALF
  22. Inhibition of interleukin-33 signaling attenuates the severity of experimental arthritis.
    Authors: Palmer G, Talabot-Ayer D, Lamacchia C, Toy D, Seemayer CA, Viatte S, Finckh A, Smith DE, Gabay C
    Arthritis Rheum., 2009;60(3):738-49.
    Species: Mouse
    Sample Type: BALF
  23. A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.
    Authors: Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G
    J. Immunol., 2009;183(2):1291-300.
    Species: Mouse
    Sample Type: BALF
  24. Macrophage migration inhibitory factor homologs of anisakis simplex suppress Th2 response in allergic airway inflammation model via CD4+CD25+Foxp3+ T cell recruitment.
    Authors: Park SK, Cho MK, Park HK, Lee KH, Lee SJ, Choi SH, Ock MS, Jeong HJ, Lee MH, Yu HS
    J. Immunol., 2009;182(11):6907-14.
    Species: Mouse
    Sample Type: BALF
  25. A TLR2 agonist in German cockroach frass activates MMP-9 release and is protective against allergic inflammation in mice.
    Authors: Page K, Ledford JR, Zhou P, Wills-Karp M
    J. Immunol., 2009;183(5):3400-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
  26. Peroxisome proliferator-activated receptor gamma agonist down-regulates IL-17 expression in a murine model of allergic airway inflammation.
    Authors: Park SJ, Lee KS, Kim SR, Min KH, Choe YH, Moon H, Chae HJ, Yoo WH, Lee YC
    J. Immunol., 2009;183(5):3259-67.
    Species: Mouse
    Sample Type: BALF
  27. Eosinophil viability is increased by acidic pH in a cAMP- and GPR65-dependent manner.
    Authors: Kottyan LC, Collier AR, Cao KH, Niese KA, Hedgebeth M, Radu CG, Witte ON, Khurana Hershey GK, Rothenberg ME, Zimmermann N
    Blood, 2009;114(13):2774-82.
    Species: Mouse
    Sample Type: Cell Lysates
  28. Modified adenine (9-benzyl-2-butoxy-8-hydroxyadenine) redirects Th2-mediated murine lung inflammation by triggering TLR7.
    Authors: Vultaggio A, Nencini F, Fitch PM, Fili L, Maggi L, Fanti P, deVries A, Beccastrini E, Palandri F, Manuelli C, Bani D, Giudizi MG, Guarna A, Annunziato F, Romagnani S, Maggi E, Howie SE, Parronchi P
    J. Immunol., 2009;182(2):880-9.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  29. TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity.
    Authors: Johnson TR, Rao S, Seder RA, Chen M, Graham BS
    Vaccine, 2009;27(23):3045-52.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  30. A severe deficiency of coagulation factor VIIa results in attenuation of the asthmatic response in mice.
    Authors: Shinagawa K, Ploplis VA, Castellino FJ
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L763-70.
    Species: Mouse
    Sample Type: BALF
  31. Celastrol suppresses allergen-induced airway inflammation in a mouse allergic asthma model.
    Authors: Kim DY, Park JW, Jeoung D, Ro JY
    Eur. J. Pharmacol., 2009;612(1):98-105.
    Species: Mouse
    Sample Type: BALF
  32. T-bet expression by dendritic cells is required for the repolarization of allergic airway inflammation.
    Authors: Heckman KL, Radhakrishnan S, Peikert T, Iijima K, McGregor HC, Bell MP, Kita H, Pease LR
    Eur. J. Immunol., 2008;38(9):2464-74.
    Species: Mouse
    Sample Type: BALF
  33. GS143, an IkappaB ubiquitination inhibitor, inhibits allergic airway inflammation in mice.
    Authors: Hirose K, Wakashin H, Oki M, Kagami S, Suto A, Ikeda K, Watanabe N, Iwamoto I, Furuichi Y, Nakajima H
    Biochem. Biophys. Res. Commun., 2008;374(3):507-11.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  34. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
    Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
    Eur. J. Pharmacol., 2008;590(1):400-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
  35. Eosinophil-nerve interactions and neuronal plasticity in rat gut associated lymphoid tissue (GALT) in response to enteric parasitism.
    Authors: O'Brien LM, Fitzpatrick E, Baird AW, Campion DP
    J. Neuroimmunol., 2008;197(1):1-9.
    Species: Rat
    Sample Type: Tissue Homogenates
  36. Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
    Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
    J. Exp. Med., 2008;205(3):699-710.
    Species: Mouse
    Sample Type: BALF
  37. Curcumin attenuates ovalbumin-induced airway inflammation by regulating nitric oxide.
    Authors: Moon DO, Kim MO, Lee HJ, Choi YH, Park YM, Heo MS, Kim GY
    Biochem. Biophys. Res. Commun., 2008;375(2):275-9.
    Species: Mouse
    Sample Type: BALF
  38. Cyclooxygenase inhibition during allergic sensitization increases STAT6-independent primary and memory Th2 responses.
    Authors: Zhou W, Newcomb DC, Moore ML, Goleniewska K, O'Neal JF, Peebles RS
    J. Immunol., 2008;181(8):5360-7.
    Species: Mouse
    Sample Type: Tissue Homogenates
  39. Novel immunomodulatory properties of berbamine through selective down-regulation of STAT4 and action of IFN-gamma in experimental autoimmune encephalomyelitis.
    Authors: Ren Y, Lu L, Guo TB, Qiu J, Yang Y, Liu A, Zhang JZ
    J. Immunol., 2008;181(2):1491-8.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  40. Pulmonary eosinophilia requires interleukin-5, eotaxin-1, and CD4+ T cells in mice immunized with respiratory syncytial virus G glycoprotein.
    Authors: Johnson TR, Rothenberg ME, Graham BS
    J. Leukoc. Biol., 2008;84(3):748-59.
    Species: Mouse
    Sample Type: Tissue Homogenates
  41. IL-27 suppresses Th2 cell development and Th2 cytokines production from polarized Th2 cells: a novel therapeutic way for Th2-mediated allergic inflammation.
    Authors: Yoshimoto T, Yoshimoto T, Yasuda K, Mizuguchi J, Nakanishi K
    J. Immunol., 2007;179(7):4415-23.
    Species: Mouse
    Sample Type: Serum
  42. Protectin D1 is generated in asthma and dampens airway inflammation and hyperresponsiveness.
    Authors: Levy BD, Kohli P, Gotlinger K, Haworth O, Hong S, Kazani S, Israel E, Haley KJ, Serhan CN
    J. Immunol., 2007;178(1):496-502.
    Species: Mouse
    Sample Type: BALF
  43. Critical role of the Fc receptor gamma-chain on APCs in the development of allergen-induced airway hyperresponsiveness and inflammation.
    Authors: Kitamura K, Takeda K, Koya T, Miyahara N, Kodama T, Dakhama A, Takai T, Hirano A, Tanimoto M, Harada M, Gelfand EW
    J. Immunol., 2007;178(1):480-8.
    Species: Mouse
    Sample Type: BALF
  44. Prolonged inhaled allergen exposure can induce persistent tolerance.
    Authors: Van Hove CL, Maes T, Joos GF, Tournoy KG
    Am. J. Respir. Cell Mol. Biol., 2007;36(5):573-84.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  45. Src homology 2 domain-containing inositol 5-phosphatase 1 deficiency leads to a spontaneous allergic inflammation in the murine lung.
    Authors: Oh SY, Zheng T, Bailey ML, Barber DL, Schroeder JT, Kim YK, Zhu Z
    J. Allergy Clin. Immunol., 2007;119(1):123-31.
    Species: Mouse
    Sample Type: BALF
  46. Liposomal retinoic acids modulate asthma manifestations in mice.
    Authors: Maret M, Ruffie C, Periquet B, Campo AM, Menevret M, Phelep A, Dziewiszek K, Druilhe A, Pretolani M
    J. Nutr., 2007;137(12):2730-6.
    Species: Mouse
    Sample Type: BALF
  47. FTY720 ameliorates oxazolone colitis in mice by directly affecting T helper type 2 functions.
    Authors: Daniel C, Sartory NA, Zahn N
    Mol. Immunol., 2007;44(13):3305-16.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  48. Regulation of allergen-induced bone marrow eosinophilopoiesis: role of CD4+ and CD8+ T cells.
    Authors: Radinger M, Bossios A, Alm AS, Jeurink P, Lu Y, Malmhall C, Sjostrand M, Lotvall J
    Allergy, 2007;62(12):1410-8.
    Species: Mouse
    Sample Type: BALF
  49. D-pinitol regulates Th1/Th2 balance via suppressing Th2 immune response in ovalbumin-induced asthma.
    Authors: Lee JS, Lee CM, Jeong YI, Jung ID, Kim BH, Seong EY, Kim JI, Choi IW, Chung HY, Park YM
    FEBS Lett., 2007;581(1):57-64.
    Species: Mouse
    Sample Type: BALF
  50. Chronic intranasal administration of mould spores or extracts to unsensitized mice leads to lung allergic inflammation, hyper-reactivity and remodelling.
    Authors: Denis O, van den Brule S, Heymans J, Havaux X, Rochard C, Huaux F, Huygen K
    Immunology, 2007;122(2):268-78.
    Species: Mouse
    Sample Type: BALF
  51. Oral administration of an immunostimulatory DNA sequence from Bifidobacterium longum improves Th1/Th2 balance in a murine model.
    Authors: Takahashi N, Kitazawa H, Iwabuchi N, Xiao JZ, Miyaji K, Iwatsuki K, Saito T
    Biosci. Biotechnol. Biochem., 2006;70(8):2013-7.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  52. Inhalation tolerance is induced selectively in thoracic lymph nodes but executed pervasively at distant mucosal and nonmucosal tissues.
    Authors: Alvarez D, Swirski FK, Yang TC, Fattouh R, Croitoru K, Bramson JL, Stampfli MR, Jordana M
    J. Immunol., 2006;176(4):2568-80.
    Species: Mouse
    Sample Type: Serum
  53. Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.
    Authors: Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J
    J. Immunol., 2005;174(4):2327-35.
    Species: Rat
    Sample Type: Cell Culture Supernates
  54. MyD88-dependent induction of allergic Th2 responses to intranasal antigen.
    Authors: Piggott DA, Eisenbarth SC, Xu L, Constant SL, Huleatt JW, Herrick CA, Bottomly K
    J. Clin. Invest., 2005;115(2):459-67.
    Species: Human
    Sample Type: Cell Culture Supernates
  55. Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
    Authors: Shibamori M, Ogino K, Kambayashi Y, Ishiyama H
    Life Sci., 2005;78(9):987-94.
    Species: Mouse
    Sample Type: BALF
  56. Immunization with gp120-depleted whole killed HIV immunogen and a second-generation CpG DNA elicits strong HIV-specific responses in mice.
    Authors: Trabattoni D, Clivio A, Bray DH, Bhagat L, Beltrami S, Maffeis G, Cesana E, Lowry P, Lissoni F, Kandimalla ER, Sullivan T, Agrawal S, Bartholomew R, Clerici M
    Vaccine, 2005;24(9):1470-7.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  57. Formulation with CpG oligodeoxynucleotides prevents induction of pulmonary immunopathology following priming with formalin-inactivated or commercial killed bovine respiratory syncytial virus vaccine.
    Authors: Oumouna M, Mapletoft JW, Karvonen BC, Babiuk LA
    J. Virol., 2005;79(4):2024-32.
    Species: Mouse
    Sample Type: BALF
  58. Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
    Authors: Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD
    J. Immunol., 2005;175(4):2589-97.
    Species: Mouse
    Sample Type: BALF
  59. Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
    Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
    Comp. Immunol. Microbiol. Infect. Dis., 2004;27(6):393-411.
    Species: Mouse
    Sample Type: Serum
  60. Vbeta14(+) T cells mediate the vaccine-enhanced disease induced by immunization with respiratory syncytial virus (RSV) G glycoprotein but not with formalin-inactivated RSV.
    Authors: Johnson TR, Varga SM, Braciale TJ, Graham BS
    J. Virol., 2004;78(16):8753-60.
    Species: Mouse
    Sample Type: Tissue Homogenates
  61. Effects of poly(ADP-ribose) polymerase inhibition on inflammatory cell migration in a murine model of asthma.
    Authors: Virag L, Bai P, Bak I, Pacher P, Mabley JG, Liaudet L, Bakondi E, Gergely P, Kollai M, Szabo C
    Med. Sci. Monit., 2004;10(3):BR77-83.
    Species: Mouse
    Sample Type: BALF
  62. Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice.
    Authors: Kobayashi T, Iijima K, Kita H
    J. Immunol., 2003;170(11):5756-63.
    Species: Mouse
    Sample Type: BALF
  63. Absence of the complement anaphylatoxin C3a receptor suppresses Th2 effector functions in a murine model of pulmonary allergy.
    Authors: Drouin SM, Corry DB, Hollman TJ, Kildsgaard J, Wetsel RA
    J. Immunol., 2002;169(10):5926-33.
    Species: Mouse
    Sample Type: BALF
  64. Structural features of peptide analogs of human histocompatibility leukocyte antigen class I epitopes that are more potent and immunogenic than wild-type peptide.
    Authors: Tangri S, Ishioka GY, Huang X, Sidney J, Southwood S, Fikes J, Sette A
    J. Exp. Med., 2001;194(6):833-46.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  65. Local anticandidal immune responses in a rat model of vaginal infection by and protection against Candida albicans.
    Authors: de Bernardis F, Santoni G, Boccanera M, Spreghini E, Adriani D, Morelli L, Cassone A
    Infect. Immun., 2000;68(6):3297-304.
    Species: Rat
    Sample Type: Vaginal Fluid
  66. Pulmonary eosinophilia and production of MIP-1alpha are prominent responses to infection with pneumonia virus of mice.
    Authors: Domachowske JB, Bonville CA, Dyer KD, Easton AJ, Rosenberg HF
    Cell. Immunol., 2000;200(2):98-104.
    Species: Mouse
    Sample Type: BALF
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Quantikine ELISA Wash Buffer 1

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Average Rating: 5 (Based on 1 review)

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  Anonymous 05/02/2017
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Summary

Sample Tested RAW 264.7 mouse monocyte/macrophage cell line,Lung tissue

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